A Pilot Series Demonstrating Fluorescence Staining of Laryngeal Papilloma Using 5‐Aminolevulinic Acid

Leunig, Andreas; Betz, Christian; Mehlmann, Michael; Stepp, Herbert; Arbogast, Susanne; Grevers, Gerhard; Baumgartner, Reinhold

doi:10.1097/00005537-200010000-00042

Cited by 10 publications

(4 citation statements)

References 17 publications

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“…This may be the result of the enhanced content of coiled and dilated blood vessels, which absorb the excitation light as well as the presence of inflammatory cells. A preliminary study by Leunig et al 17 evaluated the fluorescence characteristic of papillomas of the larynx using fluorescence‐enhancing agents and also reported a reduced fluorescence intensity.…”

Section: Discussionmentioning

confidence: 99%

Autofluorescence Endoscopy in the Diagnosis of Early Laryngeal Cancer and Its Precursor Lesions

et al. 2002

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Section: Discussionmentioning

confidence: 99%

Autofluorescence Endoscopy in the Diagnosis of Early Laryngeal Cancer and Its Precursor Lesions

et al. 2002

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“…A recent randomized multicentre phase III trial demonstrated that fluorescence-guided resection of malignant gliomas using 5 ALAinduced porphyrins enables more complete resections of contrast-enhancing tumor and leads to an improved progression-free survival [10]. The clinical usefulness of 5-ALA guided detection of tumor tissue has also been demonstrated for bladder cancer [11][12][13], malignancies of the oral cavity [14,15] and the lung [16], the female genital tract [17,18], intraperitoneal metastases [19,20] the gastrointestinal mucosa [21] and skin [22]. However, current techniques of intraoperative detection of PpIX fluorescence do not offer subcellular resolution.…”

Section: -Aminolevulinic Acid (5-ala) Is a Naturally Occurring Intermentioning

confidence: 99%

Multiphoton excitation fluorescence microscopy of 5‐aminolevulinic acid induced fluorescence in experimental gliomas

Kantelhardt

Diddens²,

Leppert

et al. 2008

Lasers Surg Med

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“…The power of elevated activity of PBGD to produce PpIX was clinically demonstrated for malignant gliomas (Stummer et al, 1998b); oral cavity cancer (Leunig et al, 2000a); colonic and gastrointestinal dysplasia (Stepp et al, 1998); peritoneal endometriosis (Hillemanns et al, 2000); laryngeal neoplasms (Leunig et al, 2000b); malignant lesions of the oesophagus (Hinnen et al, 1998); lower urinary tract tumours (Kriegmair et al, 1999); bladder cancer (Kriegmair et al, 1996); malignant mucosa in head and neck cancer (Betz et al, 1999); Barrett's oesophagus and adenocarcinoma (Stepp et al, 1998); basal and squamous cell carcinoma (Orenstein et al, 1995(Orenstein et al, , 1996(Orenstein et al, , 1997Malik et al, 1998) and coetaneous lymphoma (Orenstein et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Nuclear distribution of porphobilinogen deaminase (PBGD) in glioma cells: a regulatory role in cancer transformation?

et al. 2002

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Recently, considerable interest has been directed to red-fluorescence photodiagnosis of brain and other tumours during surgery using the protoporphyrin IX natural precursor, 5-aminolaevulinic acid. In the present study we focused on the role of the rate-limiting enzyme porphobilinogen deaminase in glioma C6 cell activity, differentiation and sub-cellular distribution. Over-expression of the human housekeeping porphobilinogen deaminase in the glioma cells, using the housekeepingporphobilinogen deaminase plasmid, induced a G1 cell cycle attenuation accompanied by increases in enzyme activity and c6 differentiation toward astrocytes. Visualisation of subcellular localisation of the porphobilinogen deaminase using the independent techniques of fluorescence immuno-staining with specific anti-human porphobilinogen deaminase antibodies and cellular expression of porphobilinogen deaminase fused to green fluorescent protein, revealed (unexpectedly) a major fraction of porphobilinogen deaminase in the nucleus and only a minor fraction in the cytoplasm. Both C and N terminals of porphobilinogen deaminase fused to green fluorescent protein revealed a major fraction of the newly synthesized fused porphobilinogen deaminase in the nucleus. Furthermore, newborn rat brain cells grown in a primary culture showed the same localisation pattern of porphobilinogen deaminase in the nuclei. Stimulation of C6 glioma cell differentiation by butyrate induced a marked decrease in porphobilinogen deaminase both in the nucleus and in the cytoplasm as determined by Western blotting and fluorescence immuno-localisation. These findings suggest a possible dual role for housekeeping porphobilinogen deaminase in fast dividing glioma cells, one related to the porphyrin synthesis pathway and another coupled to nuclear function, which might be linked to tumorigenesis.

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A Pilot Series Demonstrating Fluorescence Staining of Laryngeal Papilloma Using 5‐Aminolevulinic Acid

Cited by 10 publications

References 17 publications

Autofluorescence Endoscopy in the Diagnosis of Early Laryngeal Cancer and Its Precursor Lesions

Autofluorescence Endoscopy in the Diagnosis of Early Laryngeal Cancer and Its Precursor Lesions

Multiphoton excitation fluorescence microscopy of 5‐aminolevulinic acid induced fluorescence in experimental gliomas

Nuclear distribution of porphobilinogen deaminase (PBGD) in glioma cells: a regulatory role in cancer transformation?

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