A Pilot Analysis of Whole Transcriptome of Human Cryopreserved Sperm
Sara Stigliani,
Adriana Amaro,
Francesco Reggiani
et al.
Abstract:Sperm cryopreservation is a procedure widely used to store gametes for later use, to preserve fertility in patients prior to gonadotoxic treatments or surgery, and for sperm donation programs. The purpose of the study was to assess the impact of cryopreservation on human sperm transcriptome. Semen samples were collected from 13 normospermic men. Each sample was divided into two aliquots. The total RNA was immediately extracted from one aliquot. The second aliquot was frozen and total RNA was extracted after a … Show more
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