The affinity reaction between the antibodies to okadaic acid immobilized on the sensor's surface and the toxin in the solution has been investigated. The affinity constant, as well as the association rate constant and the dissociation rate constant, has been calculated. The unlabeled affinity sensor for the detection of okadaic acid has been developed. The calibration plot is linear in the range of concentrations of 5-500 ng mL −1 ; the limit of detection is 1.4 ng mL −1 . The developed sensor can be successfully used for the detection of toxins in real samples.