A Picric Acid Method for the Simultaneous Determination of Lactose and Sucrose in Dairy Products

Perry, Neil; Doan, F.J.

doi:10.3168/jds.s0022-0302(50)91883-2

Cited by 75 publications

(22 citation statements)

References 14 publications

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“…Analytical techniques The PMP was analyzed for fat, moisture and ash (ISI 1981), lactose and sucrose (Perry and Doan 1950). Total nitrogen content in the product was estimated by Micro-Kjeldahl method as per AOAC (1995) and considered conversion factor 6.25 for converting percent of nitrogen to protein as suggested by James (1995).…”

Section: Raw Materials and Ingredientsmentioning

confidence: 99%

Effect of varying level of dried milk proportion on formulation and reconstitution of Phirni mix powder

Kumar¹,

Paul

Kumar³

2013

J Food Sci Technol

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An investigation was undertaken to study the varying proportion of WMP to SMP (25:75, 50:50 and 75:25) for formulation of Phirni mix powder (PMP). The sensory quality of experimental Phirni increased consistently with the increase in the levels of WMP upto 50:50, thereafter, decreased significantly (p<0.01). The maximum sensory scores were obtained with a proportion of 50:50 for all sensory attributes. The textural characteristics were found significant (p<0.01) with the increase in the proportion of WMP. Further, the formulation was reconstitute by dispersing the PMP with varying levels of water (50 : 30, 50 : 40 and 50 : 50) and followed by cooked at 85±5°C for different cooking time (5,10 and 15 min). The results obtained among the reconstituted water on the sensory and textural attributes were found significant (p<0.01). The maximum sensory scores were observed at 10 min cooking time with 50: 40 proportion of PMP to water.

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Section: Raw Materials and Ingredientsmentioning

confidence: 99%

Effect of varying level of dried milk proportion on formulation and reconstitution of Phirni mix powder

Kumar¹,

Paul

Kumar³

2013

J Food Sci Technol

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“…Lactose estimation was performed as per the method of Parry and Doan [16]. Saturated picric acid (1 ml) was added to milk sample (1 ml) followed by addition of 25% w/v sodium carbonate (1 ml) and the mixture was kept in boiling water bath for 20 min.…”

Section: Estimation Of Lactosementioning

confidence: 99%

Cell Disruption Optimization and Covalent Immobilization of β-D-Galactosidase from Kluyveromyces marxianus YW-1 for Lactose Hydrolysis in Milk

Puri

Gupta

Pahuja

et al. 2009

Appl Biochem Biotechnol

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beta-D-galactosidase (EC 3.2.1.23) from Kluyveromyces marxianus YW-1, an isolate from whey, has been studied in terms of cell disruption to liberate the useful enzyme. The enzyme produced in a bioreactor on a wheat bran medium has been successfully immobilized with a view to developing a commercially usable technology for lactose hydrolysis in the food industry. Three chemical and three physical methods of cell disruption were tested and a method of grinding with river sand was found to give highest enzyme activity (720 U). The enzyme was covalently immobilized on gelatin. Immobilized enzyme had optimum pH and temperature of 7.0 and 40 degrees C, respectively and was found to give 49% hydrolysis of lactose in milk after 4 h of incubation. The immobilized enzyme was used for eight hydrolysis batches without appreciable loss in activity. The retention of high catalytic activity compared with the losses experienced with several previously reported immobilized versions of the enzyme is significant. The method of immobilization is simple, effective, and can be used for the immobilization of other enzymes.

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“…Protein was estimated by formaldehyde method (Singhal and Deshraj 1989) and lactose by picric acid method (Perry and Doon 1950). Somatic cell count was measured microscopically (IDF Bulletin 1984).…”

Section: Analysis Of Milk Samplesmentioning

confidence: 99%

Studies on cisternal and alveolar fractions & its composition and mammary health of Murrah buffaloes administered oxytocin

Bidarimath

Aggarwal

2007

Trop Anim Health Prod

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The study was conducted on 18 lactating Murrah buffaloes in 83.72 +/- 14.75 days after calving. Group I buffaloes were not injected with oxytocin and served as control. Buffaloes of Group II were injected with oxytocin @ 2.5 IU per 0.5 ml intramuscularly at the hip region for a period of 1 month while Group III buffaloes received injection of oxytocin @ 5.0 IU per ml intramuscularly at the hip region for a period of 1 month. Milk samples from three groups of buffaloes were collected after oxytocin injections at 15 days interval on 0, 15, 30 and 45 days. Blood samples were collected in heparinized tubes at 0, 15, 30 and 45 days of experiment from all three groups of buffaloes. There was no significant change in cisternal, alveolar and total milk yield by exogenous oxytocin administration. Fat % in cisternal, alveolar and total milk decreased significantly (P < 0.01) by 11.8% and 21.3% in groups II and III buffaloes, respectively. Protein % increased significantly (P < 0.01) in group III. A significant increase was observed in somatic cell counts of milk by 5.36% and 6.22% in groups II and III, respectively as compared to control group.

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A Picric Acid Method for the Simultaneous Determination of Lactose and Sucrose in Dairy Products

Cited by 75 publications

References 14 publications

Effect of varying level of dried milk proportion on formulation and reconstitution of Phirni mix powder

Effect of varying level of dried milk proportion on formulation and reconstitution of Phirni mix powder

Cell Disruption Optimization and Covalent Immobilization of β-D-Galactosidase from Kluyveromyces marxianus YW-1 for Lactose Hydrolysis in Milk

Studies on cisternal and alveolar fractions & its composition and mammary health of Murrah buffaloes administered oxytocin

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