A pico-HPLC-LIF system for the amplification-free determination of multiple miRNAs in cells

Zhang, Wenmei; Han, Zunsheng; Liang, Yingqi; Zhang, Qi; Dou, Xiangnan; Guo, Guangsheng; Wang, Xiayan

doi:10.1016/j.cclet.2020.12.007

Cited by 10 publications

(9 citation statements)

References 36 publications

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“…Nitrogen was used as a gas source to pressurize the small cylinder to realize the injection and separation of the sample. The pressure of the gas was monitored by a pressure sensor …”

Section: Methodsmentioning

confidence: 99%

“…This technique has brought unprecedented spatial and temporal resolution to molecular biology. , Ultranarrow-bore open-tubular liquid chromatography (UBOT-LC) with an inner diameter (i.d.) of approximately 2 μm or less has been used for the separation of DNA, amino acids, peptides, and proteins owing to the small sample volume requirement, low flow rate (ten to hundreds of pL/min), and high separation efficiency. − The small volume requirement also means great miniaturization potential and suitability for analyzing cytoplasm extracted from living cells. However, there are no reports on combining these two advanced techniques to analyze cytoplasm-specific substances such as nanoplastics.…”

Section: Introductionmentioning

confidence: 99%

“…The pressure of the gas was monitored by a pressure sensor. 16 Capillary Etching. A capillary with an outer diameter of 360 μm and an diameter of 1 and 0.66 μm was taken and inserted into a centrifuge tube filled with 1 mL of ultrapure water.…”

Section: ■ Introductionmentioning

confidence: 99%

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Quantitative Analysis of Nanoplastics in Single Cells by Subcellular Chromatography

et al. 2023

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The accumulation and spatial distribution of intracellular nanoplastic particles provide useful information about their spatiotemporal toxicological effects mediated by the physicochemical parameters of nanoplastics in living cells. In this study, a sample injection-transfer method was designed with an accuracy of up to femtoliters to attoliters to match the volume required for ultranarrow-bore open-tubular liquid chromatography. The separation and concentration quantification of mixed polystyrenes in different regions in living cells were achieved by directly transferring picoliter/femtoliter volumes of intracellular cytoplasm to an ultranarrow-bore open-tubular chromatographic column. The measurement of pollutant concentration in different areas of a small-volume target (single cell) was realized. This method is expected to be used in the qualitative and quantitative analyses of complex, mixed, and label-free nanoplastics (a few nm in size) in the subregions of living cells.

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“…Nitrogen was used as a gas source to pressurize the small cylinder to realize the injection and separation of the sample. The pressure of the gas was monitored by a pressure sensor …”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quantitative Analysis of Nanoplastics in Single Cells by Subcellular Chromatography

et al. 2023

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“…Among these techniques, reducing the sample volume/amount is the simplest and most direct strategy to reduce the time consumption of sample preparation and analysis. Meanwhile, device miniaturization via micromachining and microfluidics offers a great opportunity for size-reduced sample preparation and analysis [5,6].…”

Section: Introductionmentioning

confidence: 99%

Recent progress of microfluidic sample preparation techniques

Xia

2023

J of Separation Science

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Sample preparation turns out to be one of the important procedures in complex sample analysis by affecting the accuracy, selectivity, and sensitivity of analytical results. However, the majority of the conventional sample preparation techniques still suffer from time‐consuming and labor‐intensive operations. These shortcomings can be addressed by reforming the sample preparation process in a microfluidic manner. Inheriting the advantages of rapid, high efficiency, low consumption, and easy integration, microfluidic sample preparation techniques receive increasing attention, including microfluidic phases separation, microfluidic field‐assisted extraction, microfluidic membrane separation, and microfluidic chemical conversion. This review overviews the progress of microfluidic sample preparation techniques in the last 3 years based on more than 100 references, we highlight the implementation of typical sample preparation methods in the formats of microfluidics. Furthermore, the challenges and outlooks of the application of microfluidic sample preparation techniques are discussed.

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“…7,8 Compared with their wide-bore counterparts, narrow-bore capillaries have a higher separation efficiency and lower reagent consumption. In recent years, narrow-bore capillaries have been widely used in the analysis of precious biological samples, such as amino acids, 9 DNA, 10 RNA, 11 and proteins. 12 These capillaries have promising application prospects in the analysis of ultrasmall-volume samples, 13 especially in single-cell analysis.…”

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confidence: 99%

Investigation of metformin hydrochloride–bovine serum albumin interaction by narrow-bore capillary zone electrophoresis

et al. 2022

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A pico-HPLC-LIF system for the amplification-free determination of multiple miRNAs in cells

Cited by 10 publications

References 36 publications

Quantitative Analysis of Nanoplastics in Single Cells by Subcellular Chromatography

Quantitative Analysis of Nanoplastics in Single Cells by Subcellular Chromatography

Recent progress of microfluidic sample preparation techniques

Investigation of metformin hydrochloride–bovine serum albumin interaction by narrow-bore capillary zone electrophoresis

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