A Phytochemical Approach to Synthesize Silver Nanoparticles for Non-Toxic Biomedical Application and Study on their Antibacterial Efficacy

Banerjee, Pratyusha; Nath, Debjani

doi:10.15226/2374-8141/2/1/00123

Cited by 12 publications

(4 citation statements)

References 46 publications

(46 reference statements)

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“…Bacterial-removal abilities (Figure 13 and Figure 14) revealed that the AgNPs (20 mg/mL) slowed the microorganisms' development, with a mean zone of restriction of growth spanning 10-13 mm on the gram-positive bacteria than the gram-negative bacteria, while the silver nanoparticles (10 mg/mL) inhibited the spread of the microorganism with a range of 7-11 mm. This study's findings support (Pratyusha and Debjani, 2015). The idea behind silver nanoparticles' method of action is that they can enter bacterial cell walls, modify the structure of the cell membrane, and then potentially improve cell penetrability.…”

Section: Discussionsupporting

confidence: 79%

“…Comparatively, the nanoparticle's antibacterial activity was greater than the methanol water extract, which may be related to the nanoparticle's larger surface area (Lakshman et al, 2021). This might cause the nanoparticles to pass through the cytoplasmic membrane uncontrollably, which would kill the cells (Pratyusha and Debjani, 2015). Levofloxacin (a positive control) had a much greater activity than the nanoparticle and the extract.…”

Section: Discussionmentioning

confidence: 97%

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Phytochemical Screening and Synthesis of Silver Nanoparticles and Investigation of Antibacterial and Antioxidant Activities of Silybum Marianum Leaf

Unegbu,

Abdullateef,

Mishelia

2023

Preprint

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Silybum marianum is a therapeutic plant belonging to the family Asteraceae. It contains silymarin, as its principal component, and has been reported to demonstrate hepatoprotective activity. The study was aimed to investigate the phytochemical constituents, synthesis of nanoparticles, antioxidant activity and antimicrobial effects of the leaf extract. Extracts were synthesized from AgNO3 and characterized via FT-IR, U.V and XRD. The antioxidant activity was determined using DPPH, H2O2 and β-carotene assays. Similarly, all extracts expressed significant DPPH inhibition (IC50) of 5.1, 5.0, 4.7, 2.3, 4.3 and 1.1 (ug/mL), H2O2 of 1.3, 2.2, 4.7, 5.6, 4.0 and 1.0 (ug/mL) from the methanol/water extract, nanoparticles, n-Hexane, ETOAC, n-Butanol and Ascorbic acid (standard) respectively. The extract exerted scavenging activity on β-carotene with a significant IC50 inhibition of 1.5, 0.8, 5.2, 0.8, 2.7, 4.8 at 0 hour and 1.5, 0.8, 5.2, 0.8, 2.7 and 4.8 (ug/mL) after 2 hours water bath incubation at 50°C in the order of the extracts above. Additionally, nanoparticles and plant extracts significantly (p<0.0001) inhibited the growth of bacteria with mean zone of inhibition ranging 7-13 mm and 4-11 mm. This study demonstrated that extracts from different solvents of S. marianum possess significant antioxidant activity, as well as antimicrobial effects which are all concentration dependent. Thus, the result suggests that methanolic/water leaf extract of S. marianum could serve as a potential source of antioxidants and can be explored as a therapeutic agent in free radical induced diseases.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 97%

Phytochemical Screening and Synthesis of Silver Nanoparticles and Investigation of Antibacterial and Antioxidant Activities of Silybum Marianum Leaf

Unegbu,

Abdullateef,

Mishelia

2023

Preprint

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“…This may lead to an uncontrolled transport of the nanoparticles through the cytoplasmic membrane and then result in cell death. 31 On the other hand, gentamycin had better activity than the nanoparticle and the extract. Also, it was observed that on comparing the activity of the plant extract dissolved in distilled water and the other dissolved in DMSO;…”

Section: Antibacterial Studiesmentioning

confidence: 99%

“…11 The result obtained from this research is in agreement with. 31 Furthermore, the mechanism of action of silver nanoparticles is based on premise that silver nanoparticles can penetrate the cell walls of bacteria and then cause a structural change in the cell membrane which could cause an increase in cell permeability. This may lead to an uncontrolled transport of the nanoparticles through the cytoplasmic membrane and then result in cell death.…”

Section: Antibacterial Studiesmentioning

confidence: 99%

Green Synthesis of Silver Nanoparticles from Parinari curatellifolia Methanol Stem Bark Extract and Evaluation of Antioxidant and Antimicrobial Activities

2023

TJNPR

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Parinari curatellifolia Planch ex. Benth (Chrysobalanaceae) has been used traditionally for the treatment of cancer, pneumonia, fever, dressing of wounds and fractures. The green synthesized nanoparticles from phytochemicals have been utilized as effective antimicrobial and anticancer agents. The research was designed to synthesize nanoparticles from methanol stem bark extract of P. curatellifolia and to investigate its antioxidant and antibacterial activities. The stem bark was powdered and then extracted by maceration. Silver nanoparticles were synthesised using 5 mM AgNO3 solution. DPPH free radical and hydrogen peroxide scavenging assays were employed to determine the antioxidant activity. The antibacterial activity was determined using agar disk diffusion method on Staphylococcus aureus, Bacillus subtilis, Salmonella typhi and Escherichia coli. The phytochemical screening of the extract revealed the presence of saponins, flavonoids and tannins. XRD analysis of the synthesized silver nanoparticles revealed a crystalline structure with face cantered cubic orientation. The SEM revealed particle size of 1 nm and consisted of highly agglomerated particles which formed large aggregates. The IC50 of DPPH free scavenging activity of the extract, silver nanoparticles and ascorbic acid (standard) were 2.804 mg/mL, 2.367 mg/mL and 2.065 mg/mL respectively. Also, that of H2O2 against the extract, silver nanoparticles and ascorbic acid (standard) were 4.986 mg/mL, 36.56 mg/mL and 6.414 mg/mL respectively. The nanoparticles inhibited the growth of the organisms with zone of inhibition between 8-12 mm whereas that of standard antibiotic (gentamicin) ranged from18-26 mm. This therefore implies that PC extract can be used in the synthesis of nanoparticles for its use against bacteria strains and as antioxidants.

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Green synthesis of antimicrobial selenium and gold nanoparticles using Aegle marmelos fruit extract

Sarkar

Mena

Kumar

et al. 2022

Vegetos

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A Phytochemical Approach to Synthesize Silver Nanoparticles for Non-Toxic Biomedical Application and Study on their Antibacterial Efficacy

Cited by 12 publications

References 46 publications

Phytochemical Screening and Synthesis of Silver Nanoparticles and Investigation of Antibacterial and Antioxidant Activities of Silybum Marianum Leaf

Phytochemical Screening and Synthesis of Silver Nanoparticles and Investigation of Antibacterial and Antioxidant Activities of Silybum Marianum Leaf

Green Synthesis of Silver Nanoparticles from Parinari curatellifolia Methanol Stem Bark Extract and Evaluation of Antioxidant and Antimicrobial Activities

Green synthesis of antimicrobial selenium and gold nanoparticles using Aegle marmelos fruit extract

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