A physiological and structural study of neuron types in the cochlear nucleus. II. Neuron types and their structural correlation with response properties

Abstract

The present study examined the morphological cell types of neurons labeled with intracellular horseradish peroxidase injections, many of them following electrophysiological recordings in the cochlear nucleus of gerbils and chinchillas. Most of the subdivisions and neuronal types previously described in the cat were identified in the present material, including spherical and globular bushy cells, stellate, bushy multipolar, elongate, octopus, and giant cells in the ventral cochlear nucleus, and a cartwheel cell in the dorsal cochlear nucleus. In many cases these structurally distinct neurons were correlated with their characteristic responses to stimulation by sound or intracellular injection of depolarizing current. The dendritic terminals of the elongate, antenniform, and clavate cells of the posteroventral cochlear nucleus link each of these cell types with neighboring structures in distinct patterns, which may provide a basis for differences in synaptic organization. These cell types differ from each other and from the stellate cells of the anteroventral cochlear nucleus. Despite their heterogeneous morphology, most of these neurons had a regular discharge in response to stimulation (choppers). Irregularly firing neurons (primary-like) had very different structures, e.g., the spherical and globular bushy cells and the bushy multipolar neuron. They, too, represent a heterogeneous population. An onset neuron was identified as an octopus cell. This paper compares the morphological observations with the electrophysiological properties of different cell types reported in a companion paper (Feng et al. [1994] J. Comp. Neurol.). Together, these findings imply that response properties may be partially independent of neuronal structure. Morphologically distinct neurons can generate similar temporal patterns in response to simple acoustic stimuli. Nevertheless, the synaptic organization of these different neuron types, including their connections, would be expected to affect or alter the cells' responses to appropriate stimuli. The possibility is raised that membrane properties and synaptic organization complement and interact with each other.

Interaction of Excitation and Inhibition in Anteroventral Cochlear Nucleus Neurons That Receive Large Endbulb Synaptic Endings

“…This was done because the rostral AVCN includes a nearly homogenous population of neurons, the SBCs (Irvine, 1986;Cant and Hyson, 1992;Ostapoff et al, 1994;Bazwinsky et al, 1999), which in this part represent frequencies below 3-6 kHz and show the largest endbulb terminals (Rouiller et al, 1986). Therefore a higher percentage of PP recordings could be expected.…”

Identification of cell types in brain slices of the inferior colliculus

“…In the cochlear nucleus, discharge patterns correlate well with speci®c morphological cell types. 35,49,50 Efforts to characterize the intrinsic electrical properties of IC neurons are, however, just beginning 21,58,59 and discharge patterns have not been correlated with morphology. A more detailed analysis is needed to test the hypothesis that cell types are indicated by discharge pattern, and whether functionally de®ned cell types are correlated with other morphological properties such as Nissl pattern, cell body size or shape, dendritic branching, dendritic spines, or synaptic distribution.…”

Direct comparison between properties of adaptation of the auditory nerve and the ventral cochlear nucleus in response to repetitive clicks

“…Osen, 1969;Brawer et al, 1974;Cant and Morest, 1979;Hackney et al, 1990). Intracellular single-unit studies have associated these three cell types with primary-like, primary-like with notch, and chopper responses, respectively (Rhode et al, 1983;Rouiller and Ryugo, 1984;Smith and Rhode, 1987;Smith et al, 1991Smith et al, , 1993Ostapoff et al, 1994; for a review see Rouiller, 1997;Romand and Avan, 1997). At least the gross envelope of these responses (as implied in part by their names) is comparable to the one typically observed in AN fibers.…”