A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering

Wei, Lanting; Wang, Xiang; Fu, Junjie; Yin, Jian; Hu, Jing

doi:10.1016/j.colsurfa.2023.131410

Cited by 12 publications

(7 citation statements)

References 60 publications

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“…This enhanced effect may be attributed to the fact that chitosan is a cationic polysaccharide that has a strong affinity for mammalian cells with negatively charged surfaces (Mazia et al, 1975;Ribas Fonseca et al, 2020). Additionally, the amino acid groups on CS contribute to enhanced cell adhesion and expansion, consequently promoting cell proliferation on Gel-CS (Wei et al, 2023).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, we assessed the modulus and viscosity of four hydrogels before and after Gel crosslinking with TGase, denoted as the 'No crosslinking' group and 'Gel crosslinking' group, respectively, according to 2.5. The energy storage modulus (denoted as G') and the loss modulus (denoted as G") are key parameters characterizing the elasticity and viscosity of substances, respectively (Oroian et al, 2018;Wei et al, 2023). Regardless of whether or not the Gel was crosslinked in the hydrogels, the G' and G" curves of pure Gel, Gel-SA, Gel-CS, and Gel-GG hydrogels demonstrated no intersection points, and the G' values consistently exceeded the G" values, indicating that these hydrogels were able to form stable elastic structures over a broad spectrum of shear frequencies (Figures 5C,D).…”

Section: Identification Of Candidate Bio-inks For 3d Bioprinting Of F...mentioning

confidence: 99%

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Highly efficient isolation and 3D printing of fibroblasts for cultured meat production

Yang,

Fei,

Wang

et al. 2024

Front. Sustain. Food Syst.

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Fibroblasts are important components of animal tissues such as muscle and skin, as they are the major producers of various matrix proteins. Matrix proteins such as collagen play an important role in meat products by providing unique nutrition, texture, and flavor. Cultured meat is an innovative meat alternative produced by culturing animal cells, but currently, relatively few studies have been conducted using fibroblasts as seed cells for cultured meat manufacturing. In this work, we first developed an innovative digestion-friction method for isolating fibroblasts from porcine skin efficiently and cost-effectively. After optimizing the enzymatic digestion and physical friction conditions, 2.39 ± 0.28 × 105 fibroblasts were obtained from 1 cm2 of porcine skin tissue, which was about 9 times higher than the conventional tissue explant method. In addition, we identified an edible bio-ink composed of gelatin and chitosan that has good printing properties and supports fibroblast adhesion and growth. Furthermore, we fabricated fibroblast-based cultured meat by 3D printing with an initial cell density of 1.0 × 107 mL−1 and evaluated its texture and nutritional properties. This work provides valuable insights and references for introducing fibroblasts into the production of cultured meat that is more comparable to structured animal meat.

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Section: Discussionmentioning

confidence: 99%

Section: Identification Of Candidate Bio-inks For 3d Bioprinting Of F...mentioning

confidence: 99%

Highly efficient isolation and 3D printing of fibroblasts for cultured meat production

Yang,

Fei,

Wang

et al. 2024

Front. Sustain. Food Syst.

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“…These results might be due to the salting-out effect of lower concentrations of Na 2 SO 4 solutions, which removed excess free water to produce a denser and regular hydrogel with small pores. 26,36,37 The higher the concentration of Na 2 SO 4 solution, the stronger the intermolecular hydrogen bonding of PVA chain. Meanwhile, the strength of entire molecular chain skeleton increased as well as the pore walls of PVA-Na became thicker.…”

Section: Morphological Characterizationmentioning

confidence: 99%

“…On the one hand, Na 2 SO 4 solution had salting-out effect, which could drain excess free water from the inside of PVA hydrogels and make their pores denser. 26 On the other hand, CaCO 3 could be uniformly dispersed in PVA hydrogels, and large amounts of carbon dioxide were generated by reacting with acids, thus, improving the coherence of the three-dimensional network structure and increasing the pore size of porous PVA hydrogels. 27 In this work, porous PVA hydrogels were fabricated by cyclic freeze-thaw and freeze-drying using Na 2 SO 4 and CaCO 3 as porogens.…”

Section: Introductionmentioning

confidence: 99%

Pore structure evolution and adsorption behaviors of porous poly (vinyl alcohol) hydrogel prepared using Na₂SO₄ and CaCO₃ as porogens

Xiang,

Yu,

Xiang

et al. 2024

Polymer Engineering & Sci

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In this work, porous poly (vinyl alcohol) (PVA) hydrogels with different pore sizes were fabricated using inorganic non‐metallic porogens by an environmentally friendly method, providing a potential solution for the field of wastewater treatment. PVA hydrogels have gained much attention in the field of wastewater treatment due to their excellent adsorption performance and porous network structure. However, the employment of inorganic non‐metallic salts as porogens for PVA hydrogels has received limited attention so far. In this study, porous PVA hydrogels were prepared by freeze‐thawing and freeze‐drying with sodium sulfate (Na2SO4) and calcium carbonate (CaCO3) as porogens. PVA‐Na exhibited denser network structure with the concentration of Na2SO4 solution increased, and the tensile stress was enhanced by about 45 times. Meanwhile, the tensile stress of PVA‐Ca was improved significantly from 0.06 to 1.67 MPa, and the network structure became looser after adding CaCO3. Moreover, a comprehensive investigation was conducted on the adsorption performance of porous PVA hydrogel for Congo red (CR). Finally, five adsorption models were fitted to investigate the adsorption mechanism of porous PVA hydrogels, in which the maximum adsorption capacity of CR by PVA‐Na‐0.5 was predicted to be 215 mg/g by the Langmuir isothermal model fitting.Highlights The structure of PVA hydrogels was adjusted by Na2SO4 and CaCO3, respectively. The tensile stress of PVA‐Na with dense network structure increased 45 times. Compared to PVA‐FD, swelling equilibrium time of PVA‐Na reduced from 24 to 6 h. Equilibrium swelling rate of PVA‐Ca declined by half in comparison to PVA‐FD. Langmuir isothermal model predicted qmax of 215 mg/g for CR by PVA‐Na‐0.5.

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“…Improved mechanical properties of traditional hydrogels, including strength, are significantly enhanced in the double network hydrogel, while maintaining features like biocompatibility, water absorption, and transparency [17]. Double network hydrogels are widely employed in various fields, including biological tissue engineering [18], medical materials [19], agriculture and forestry [20], environmental protection [21], sensor materials, among others. Recently, there has been an increasing level of activity in the research on double network hydrogels characterized by good biocompatibility and mechanical properties [22].…”

Section: Introductionmentioning

confidence: 99%

An Innovative High-Strength Double-Network Hydrogel for Use as a Drilling Fluid Plugging Agent

He,

Guo,

Bai

et al. 2024

Gels

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The problem of wellbore leakage is a key challenge in the petroleum industry, limiting drilling progress and increasing drilling costs. Plugging agents play a role in repairing leaks and fractures; however, traditional plugging materials generally have low mechanical strength, poor adaptability to permeable strata, limited water absorption and expansion capabilities, and poor temperature and salt resistance. To address these limitations, a pioneering polyacrylic acid-polyacrylamide (PAA/PAM) double-network hydrogel was synthesized through aqueous solution polymerization in this study. Its strength, water absorption, expansion, temperature resistance, salt resistance, and plugging effectiveness were comprehensively evaluated. The results demonstrate that good mechanical performance is exhibited by the synthesized hydrogel, capable of withstanding a maximum stress of approximately 3.5 MPa at a 90% strain. Excellent water absorption and expansion are observed in the synthesized double-network hydrogel, with a maximum expansion of 6 times within 30 min and 8 times after 2 h. Test results show that the hydrogel had good temperature resistance and salt resistance, maintaining a strength grade E within the experimental range. The simulated evaluation of the plugging experiment indicates that, under conditions of 130 °C and 6 MPa, the leakage rate of the drilling fluid is maintained below 5 mL/min when the double-network hydrogel is utilized. From the above experimental results, it can be illustrated that excellent mechanical properties, impressive water absorption, and expansion capabilities are exhibited by the synthesized double-network hydrogel. Furthermore, the high-temperature resistance and salt resistance of the double-network hydrogel were also demonstrated. Therefore, In comparison to traditional plugging materials, significant promise is held by this newly synthesized double-network hydrogel material as a plugging agent in drilling fluids.

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A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering

Cited by 12 publications

References 60 publications

Highly efficient isolation and 3D printing of fibroblasts for cultured meat production

Highly efficient isolation and 3D printing of fibroblasts for cultured meat production

Pore structure evolution and adsorption behaviors of porous poly (vinyl alcohol) hydrogel prepared using Na₂SO₄ and CaCO₃ as porogens

An Innovative High-Strength Double-Network Hydrogel for Use as a Drilling Fluid Plugging Agent

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A physically cross-linked double network polysaccharides/Ca2+ hydrogel scaffold for skeletal muscle tissue engineering

Cited by 12 publications

References 60 publications

Highly efficient isolation and 3D printing of fibroblasts for cultured meat production

Highly efficient isolation and 3D printing of fibroblasts for cultured meat production

Pore structure evolution and adsorption behaviors of porous poly (vinyl alcohol) hydrogel prepared using Na2SO4 and CaCO3 as porogens

An Innovative High-Strength Double-Network Hydrogel for Use as a Drilling Fluid Plugging Agent

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Product

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About

Pore structure evolution and adsorption behaviors of porous poly (vinyl alcohol) hydrogel prepared using Na₂SO₄ and CaCO₃ as porogens