A physical map of the Escherichia coli bio operon

Szybalski, Elizabeth H.; Szybalski, Waclaw

doi:10.1016/0378-1119(82)90193-7

Cited by 19 publications

(8 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Very recently, the bioconversion of DTB to biotin by a cell-free extract of an Escherichia coli transformant carrying its bioB gene has been reported [17]. The arrangement of bio gene clusters in E. coli and B. sphaericus are known to be different: in the former, genes related to the bioconversion of pimelyl-CoA to biotin are encoded in a single operon, bioABFCD [18,19], while the latter has two gene clusters, bioDAYB and bioXWF [20].…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of dethiobiotin to biotin by a cell-free system of a bioYB transformant of Bacillus sphaericus

Fujisawa

1993

FEMS Microbiology Letters

View full text Add to dashboard Cite

The bioconversion of dethiobiotin to biotin has been demonstrated by cell‐free extracts of a biotin‐hyperproducing Bacillus sphaericus mutant transformed with an expression vector for two bio genes: bioB, encoding biotin synthase; and bioY, the function of which remains unknown. The reaction mixture for the biosynthesis of biotin contained yeast extract, l‐cysteine and sucrose as well as dethiobiotin. The cell‐free extracts prepared from protoplasts of the transformant with a French press at not more than 1000 kg cm−2 showed appreciable biotin synthase activities. An unusually high protein concentration (more than 18 mg ml−1) in the reaction mixture was required for the enzyme activity, while cell‐free extracts of less than 10 mg protein ml−1 hardly exhibited detectable activities. The product from dethiobiotin in a reaction mixture was identified as biotin by bioautography with Lactobacillus plantarum.

show abstract

Section: Introductionmentioning

confidence: 99%

Bioconversion of dethiobiotin to biotin by a cell-free system of a bioYB transformant of Bacillus sphaericus

Fujisawa

1993

FEMS Microbiology Letters

View full text Add to dashboard Cite

show abstract

“…o. as described by Szybalski and Szybalski (1982 The proximity of the bio operon to ).. on the E. coli genome has facilitated the isolation of numerous bio transducing ).. phages, many of which have been characterized genetically. These have been utilized to construct a physical map of the E. coli bio operon (Szybalski and Szybalski, 1982). The first heteroduplex mapping study of the bio operon was done by Hradecna and Szybalski (1969;errata, 1970).…”

Section: The Bio Genes Of E Coli Location and Structure Of The E Comentioning

confidence: 99%

“…1975). and thus constitute a single operon (Szybalski and Szybalski, 1982). Figure 2 shows the gene order in the bio operon.…”

Section: The Bio Genes Of E Coli Location and Structure Of The E Comentioning

confidence: 99%

The Production of Biotin by Genetically Modified Micro-organisms

Brown

Kamogawa

1991

Biotechnology and Genetic Engineering Reviews

View full text Add to dashboard Cite

“…The bio operon is located in the above order between the bacteriophage A attachment site and the uvrB gene loci at approximately 17 minutes on the E. coli genetic map of Bachmann (1987). The two divergent bio transcriptional units are controlled by a single operator, 0 (Ketner and Campbell, 1975), and thus constitute a single operon (Szybalski and Szybalski, 1982). Figure 2 shows the gene order in the bio operon.…”

Section: The Bio Genes Of E Coli Location and Structure Of The E Comentioning

confidence: 99%

“…These have been utilized to construct a physical map of the E. coli bio operon (Szybalski and Szybalski, 1982). The first heteroduplex mapping study of the bio operon was done by Hradecna and Szybalski (1969;errata, 1970).…”

Section: Physical Map Of the E Coli Bio Operonmentioning

confidence: 99%