A Phylogeographical Analysis of the Beetle Pest Species Callosobruchus chinensis (Linnaeus, 1758) in China

Abstract

Callosobruchus chinensis (Coleoptera Bruchidae), is a pest of different varieties of legumes. In this paper, a phylogeographical analysis of C. chinensis was conducted to provide knowledge for the prevention and control of C. chinensis. A total of 224 concatenated mitochondrial sequences were obtained from 273 individuals. Suitable habitat shifts were predicted by the distribution modelling (SDM). Phylogeny, genetic structure and population demographic history were analyzed using multiple software. Finally, the least-cost path (LCP) method was used to identify possible dispersal corridors and genetic connectivity. The SDM results suggested that the distribution of C. chinensis experienced expansion and contraction with changing climate. Spatial distribution of mtDNA haplotypes showed there was partial continuity among different geographical populations of C. chinensis, except for the Hohhot (Inner Mongolia) population. Bayesian skyline plots showed that the population had a recent expansion during 0.0125 Ma and 0.025 Ma. The expansion and divergent events were traced back to Quaternary glaciations. The LCP method confirmed that there were no clear dispersal routes. Our findings indicated that climatic cycles of the Pleistocene glaciations, unsuitable climate and geographic isolation played important roles in the genetic differentiation of C. chinensis. Human activities weaken the genetic differentiation between populations. With the change in climate, the suitable areas of C. chinensis will disperse greatly in the future.

Phylogeographic investigation of the bladder grasshopperBullacris unicolor(Orthoptera Pneumoroidea) in South Africa

“…Male B. unicolor show a low dispersal ability and move only relatively short distances (Alexander & van Staaden 1989). The development of population structure is greatly influenced by factors that affect dispersal, such as historical variance and geographic distance coupled with differences in habitat discontinuity and dispersal ability (Serrana et al 2019a;Wang et al 2022).…”

Stored product pest Nemapogon variatella (Clemens): Identification of sex pheromone, putative antagonist and mitotype characterization

“…Based on the intraspecific variability of N. variatella mtDNA CO1-5′, the calculated high haplotype diversity (Hd = 0.796), but low nucleotide diversity (Pi = 0.007) indicate a high number of closely related mitotypes and suggest that N. variatella may have undergone a recent population expansion after a period of low effective population size (Avise, 2000;Wei et al, 2013). It is common among other stored product pest species as well, for example, the cowpea aphid Aphis craccivora Koch (Hemiptera: Aphididae; Wongsa et al, 2017), the adzuki bean weevil Callosobruchus chinensis Linnaeus (Coleoptera: Bruchidae; Wang, Li, et al, 2022). However, we cannot confirm or deny this assumption explained by the intensive population mixing due to intercontinental agricultural trade and global travel (Prasanna et al, 2022) during the last century, which inevitably caused the spread and mixing of mitotypes without geographic isolation.…”

“…However, even the best studied mitochondrial DNA cytochrome c oxidase subunit 1 (mtDNA CO1), serving not only as the core of a global bioidentification system for animals (Hebert et al, 2003) but also as the base of genetic diversity research (e.g. Fu et al, 2020; Thangaraj et al, 2019; Wang, Li, et al, 2022; Wongsa et al, 2017), has not been studied enough in N. variatella . So far, only single specimens of this species have been analysed in the framework of other studies, such as in the evaluation of species richness of Canadian insects (Hebert et al, 2016), the reconstruction of lepidopteran phylogeny (Mutanen et al, 2010), the detection of non‐monophyly in the CO1 data of European butterflies and moths (Mutanen et al, 2016), the replenishment of DNA barcode library for Italian and Austrian Lepidoptera (Huemer & Hebert, 2016), and the detection of lepidopteran species characterized by Holarctic distribution (Landry et al, 2013).…”