A phylogenetic study of the genus Wiborgia (Crotalarieae, Fabaceae)

Moiloa, Ntwai; Chimphango, S.B.M.; Muasya, A. Muthama

doi:10.1016/j.sajb.2018.02.005

Cited by 4 publications

(5 citation statements)

References 31 publications

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“…Polhillia , Wiborgia and Wiborgiella species belong to the family Leguminosae and tribes Genisteae and Crotalarieae 1 – 3 . They are endemic to the Cape fynbos biome, recognized as one of the richest areas of flowering plants in the world 4 – 6 .…”

Section: Introductionmentioning

confidence: 99%

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Ecological adaptation and phylogenetic analysis of microsymbionts nodulating Polhillia, Wiborgia and Wiborgiella species in the Cape fynbos, South Africa

Mpai

Jaiswal

Cupido

et al. 2021

Sci Rep

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Polhillia, Wiborgia and Wiborgiella species are shrub legumes endemic to the Cape fynbos of South Africa. They have the ability to fix atmospheric N2 when in symbiosis with soil bacteria called ‘rhizobia’. The aim of this study was to assess the morpho-physiological and phylogenetic characteristics of rhizobia associated with the nodulation of Polhillia, Wiborgia and Wiborgiella species growing in the Cape fynbos. The bacterial isolates from root nodules consisted of a mixture of fast and intermediate growers that differed in colony shape and size. The isolates exhibited tolerance to salinity (0.5–3% NaCl) and pH (pH 5–10) and different antibiotic concentrations, and could produce 0.51 to 51.23 µg mL−1 of indole-3-acetic acid (IAA), as well as solubilize tri-calcium phosphate. The ERIC-PCR results showed high genomic diversity in the rhizobial population and grouped them into two major clusters. Phylogenetic analysis based on 16S rRNA, atpD, glnII, gyrB, nifH and nodC gene sequences revealed distinct and novel evolutionary lineages related to the genus Rhizobium and Mesorhizobium, with some of them being very close to Mesorhizobium australicum. However, the phylogenetic analysis of glnII and nifH genes of some isolates showed incongruency.

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Section: Introductionmentioning

confidence: 99%

“…They are endemic to the Cape fynbos biome, recognized as one of the richest areas of flowering plants in the world 4 – 6 . These legumes have bright yellow and/or white flowers, which are a major attraction for tourists 3 , 7 . They also contribute to the fertility of fynbos soil through N 2 fixation with native soil rhizobia.…”

Section: Introductionmentioning

confidence: 99%

Ecological adaptation and phylogenetic analysis of microsymbionts nodulating Polhillia, Wiborgia and Wiborgiella species in the Cape fynbos, South Africa

Mpai

Jaiswal

Cupido

et al. 2021

Sci Rep

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“…with >99% sequence identity to Ensifer meliloti and Ensifer kummerowiae , strain WR52 as Mesorhizobium sp., both originating from different nodules of W. monoptera . To our knowledge, only a single study analyzed the rhizobial community associated with the genus Wiborgia except W. monoptera , indicating a difference in specificity between Wiborgia species depending on their geographical distribution, and a preference for Rhizobium and Mesorhizobium ( Moiloa, 2016 ). Other isolates, WR23 or WR93 from D. barbatum or Chamaecrista sp., respectively, and WR74 or WR96 from Leobordea digitata or I. alternans , respectively, were members of the genus Bradyrhizobium .…”

Section: Resultsmentioning

confidence: 99%

Root Nodule Rhizobia From Undomesticated Shrubs of the Dry Woodlands of Southern Africa Can Nodulate Angolan Teak Pterocarpus angolensis, an Important Source of Timber

et al. 2021

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Pterocarpus angolensis, a leguminous tree native to the dry woodlands of Southern Africa, provides valuable timber, but is threatened by land conversion and overharvesting while showing limited natural regeneration. Nitrogen-fixing root nodule symbionts that could improve establishment of young seedlings have not yet been described. Therefore, we investigated the ability of P. angolensis to form nodules with a diverse range of rhizobia. In drought-prone areas under climate change with higher temperatures, inoculants that are heat-tolerant and adapted to these conditions are likely to be of advantage. Sources of bacterial isolates were roots of P. angolensis from nurseries in the Kavango region, other shrubs from this area growing near Pterocarpus such as Indigofera rautanenii, Desmodium barbatum, Chamaecrista sp., or shrubs from drought-prone areas in Namaqualand (Wiborgia monoptera, Leobordea digitata) or Kalahari (Indigofera alternans). Only slight protrusions were observed on P. angolensis roots, from which a non-nodulating Microbacterium sp. was isolated. Rhizobia that were isolated from nodules of other shrubs were affiliated to Bradyrhizobium ripae WR4T, Bradyrhizobium spp. (WR23/WR74/WR93/WR96), or Ensifer/Mesorhizobium (WR41/WR52). As many plant growth-promoting rhizobacteria (PGPR), nodule isolates produced siderophores and solubilized phosphate. Among them, only the Bradyrhizobium strains nodulated P. angolensis under controlled conditions in the laboratory. Isolates were further characterized by multilocus sequence analysis and were found to be distant from known Bradyrhizobium species. Among additional reference species tested for nodulation on P. angolensis, Bradyrhizobium vignae 7-2T and Bradyrhizobium namibiense 5-10T from the Kavango region of Namibia as well as Bradyrhizobium elkanii LMG6234T and Bradyrhizobium yuanmingense LMG21728T induced nitrogen-fixing nodules, while Bradyrhizobium diazoefficiens USDA110T and Bradyrhizobium tropiciagri SEMIA6148T did not. This suggests a broad microsymbiont range from Bradyrhizobium japonicum and B. elkanii lineages. Phylogenetic analysis of nodC genes indicated that nodulating bradyrhizobia did not belong to a specific symbiovar. Also, for I. rautanenii and Wiborgia, nodule isolates B. ripae WR4T or Mesorhizobium sp. WR52, respectively, were authenticated. Characterization of symbionts inducing effective root nodules in P. angolensis and other shrubs from Subsahara Africa (SSA) give insights in their symbiotic partners for the first time and might help in future to develop bioinoculants for young seedlings in nurseries, and for reforestation efforts in Southern Africa.

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“…Wiborgia Thunb. (Fabaceae), with 10 species all endemic to the region of Greater Cape in South Africa, comprises shrubs of 0.5–3 m, with a distinct ascending to erect habit and shoots terminating in rigid thorns (Moiloa & al., 2018).…”

Section: Two Legumes Species Described As Polygala By Nl Burmanmentioning

confidence: 99%

Typifications of the N.L. Burman and Linnaean names in Polygala, Polygalaceae

Pastore

Martinez

2021

TAXON

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The historical context and typifications of a number of not yet typified Linnaean names in Polygala (P. acutifolia, P. aspalatha, P. brasiliensis, P. chamaebuxus, P. microphylla, P. penaea, P. sibirica, P. theezans) are presented, along with the identities of P. acutifolia and P. theezans. Bartolomeo di Panigai, a cartographer in the Portuguese boundary commission sent on an expedition to establish borders between Portugal and Spain in accordance with the Treaty of Madrid (signed in 1750), is suggested as the likely collector of P. aspalatha and P. brasiliensis. Three of N.L. Burman's names (P. pinnata, P. thea, P. triphylla) are revised, and two new combinations, Indigofera pinnata and Wiborgia triphylla, the legume species originally described by N.L. Burman in genus Polygala, are presented.

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A phylogenetic study of the genus Wiborgia (Crotalarieae, Fabaceae)

Cited by 4 publications

References 31 publications

Ecological adaptation and phylogenetic analysis of microsymbionts nodulating Polhillia, Wiborgia and Wiborgiella species in the Cape fynbos, South Africa

Ecological adaptation and phylogenetic analysis of microsymbionts nodulating Polhillia, Wiborgia and Wiborgiella species in the Cape fynbos, South Africa

Root Nodule Rhizobia From Undomesticated Shrubs of the Dry Woodlands of Southern Africa Can Nodulate Angolan Teak Pterocarpus angolensis, an Important Source of Timber

Typifications of the N.L. Burman and Linnaean names in Polygala, Polygalaceae

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