A Phylogenetic Analysis of Doronicum (Asteraceae, Senecioneae) Based on Morphological, Nuclear Ribosomal (ITS), and Chloroplast (trnL-F) Evidence

Abstract

A phylogenetic analysis of the Old World genus Doronicum (26 species, 4 subspecies) based on sequence data of the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA, the chloroplast spacer trnL-F, and morphology is presented. Congruence among the three data sets was explored by the computing of several indices, all of which suggest homogeneity between only the two molecular matrices. We argue that the morphological data set contains poor phylogenetic signal and advocate simultaneous analysis of the three data sets (total evidence approach) so that morphological characters are tested for homology by congruence with molecular data. The resulting phylogenetic hypothesis allows several well-supported conclusions including the placement of a Corsican endemic (D. corsicum), sister to the remainder of the genus, and the inference that an early southern European or Mediterranean diversification took place in the genus. Shifts in morphological characters (e.g., homocarpy to heterocarpy) are confirmed to have evolved several times. Results from comparative studies of sequence data of the chloroplast gene ndhF support inclusion of Doronicum in tribe Senecioneae.

Developmental Control and Plasticity of Fruit and Seed Dimorphism in Aethionema arabicum

“…Moreover, sensory elements are required when morph development is regulated in an environmentally dependent manner. Nevertheless, heteromorphism evolved many times independently (Fernández et al, 2001;Imbert, 2002;Cruz-Mazo et al, 2009), suggesting that its genetic basis is rather simple. A deep understanding of heteromorphism at the phenotypic level is crucial for subsequent molecular studies.…”

Thymol derivatives from essential oil of Doronicum corsicum L.

“…A phylogenetic analysis of the genus Doronicum based on three different datasets, the morphology, the internal transcribed spacers (ITS) of the nuclear ribosomal (DNA) and the chloroplast spacer region trnL-F, has recently been reported. 3 The most outstanding result of this study was the basal position of D. corsicum.…”

“…The PCI-NH 3 − at m/z 87. Thus, the molecular mass and the mass of the acid part of this compound were deduced from EI-and CI-mass spectra.…”

Phylogenetic relationships of Pterocarya (Juglandaceae) with an emphasis on the taxonomic status of Iranian populations using ITS and trnH-psbA sequence data

“…Molecular sequence data along with phylogenetic analyses are increasingly being used for species identification and evolutionary inference in many plant species. The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA has been frequently used to determine phylogenetic relationships in plants (Potter et al 2000;Fernández et al 2001;Samuel et al 2003;Fukuda et al 2011;Yousefzadeh et al 2012). Due to the small size (approximately 700 base pairs (bp)) and adequate variation in (the rate of nucleotide substitutions) universal primers as well as easy amplification, this region was selected for use in a phylogenetic analysis of plants at lower taxonomic levels.…”