A photo-sensitizable phage for multidrug-resistant <i>Acinetobacter baumannii</i> therapy and biofilm ablation

Ran, Bei; Yuan, Yuyu; Xia, Weiguo; Li, Mingle; Yao, Qichao; Wang, Zuokai; Wang, Lili; Li, Xiaoyü; Xu, Yongping; Peng, Xiaojun

doi:10.1039/d0sc04889e

Cited by 51 publications

(36 citation statements)

References 41 publications

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“…Due to acid microenvironment of S. mutans biofilm, the Ce6 was protonated and released; then, SiO 2 -P Ce6-IL changed to positive charged SiO 2 -P IL + . The biofilm had anions such as carboxyl, phosphoryl, and glycerate at the proteins or polysaccharides would produce negatively charge [19]. The electrostatic interactions would occur between SiO 2 -P IL + and S. mutans biofilm.…”

Section: Resultsmentioning

confidence: 99%

Multiclawed SiO₂ Nano‐Antibacterial Agent Based on Charge Inversed Ce6 Ionic Liquid Polymers for Combating Oral Biofilm Infection

Jiao

Teng²,

Zhan

et al. 2022

Journal of Nanomaterials

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Photodynamic antimicrobial chemotherapy (PACT) is a promising therapy against biofilm infection. However, due to the saliva clearance and obstacle of biofilm, the photosensitizer is difficult to concentrate in the infection site; then, the PACT is less effective on oral biofilm infection. In this article, we report a special nano-antibacterial agent (SiO2-PCe6-IL) to solve the bottleneck problem of PACT in treatment of oral biofilm infections. The SiO2-PCe6-IL was composed of SiO2 and poly ionic liquid photosensitizer (PCe6-IL) and had tri-fold features of eliminate biofilm infection: high binding ability, breaking biofilm barriers, and enrichment photosensitizer in infection site. In oral biofilm, the SiO2-PCe6-IL changed to SiO2-PIL+ like claws of octopus that could hold tightly with biofilm. Then, the poly-dodecyl on the SiO2-PIL+ broke down the barrier of biofilm. The results of HR-MS and zeta potential indicated that SiO2-PCe6-IL could change to positive (SiO2-PIL+) in acidic environment. The interaction forces and morphology results proved that the SiO2-PIL+ had a higher affinity to biofilm and could destroy the biofilm structure. Then, the photosensitizer was enriched in biofilm at sites of infection. The in vitro and in vivo experiments showed that SiO2-PCe6-IL could effectively eradicate oral biofilm infections and control of dental caries.

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Section: Resultsmentioning

confidence: 99%

Multiclawed SiO₂ Nano‐Antibacterial Agent Based on Charge Inversed Ce6 Ionic Liquid Polymers for Combating Oral Biofilm Infection

Jiao

Teng²,

Zhan

et al. 2022

Journal of Nanomaterials

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“…While phage cocktail combined with levofloxacin and amikacin, did not act synergistically ( 325 ). An excellent method of inhibiting A. baumannii biofilm was devised by Ran et al ( 326 ) by combining photodynamic bacteriophages (ABP) and Nile blue photosensitizers (NB). NB photosensitizer possessing sulfur atom displayed ROS production ability.…”

Section: Strategies Of Preventing a Baumannii Biof...mentioning

confidence: 99%

“…NB photosensitizer possessing sulfur atom displayed ROS production ability. Both in vitro and in vivo experiments proved that NB-phage bioconjugate (APNB) could bind to the main components of biofilms and reduce drug resistance caused by biofilms ( 326 ).…”

Section: Strategies Of Preventing a Baumannii Biof...mentioning

confidence: 99%

Convergence of Biofilm Formation and Antibiotic Resistance in Acinetobacter baumannii Infection

et al. 2022

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Acinetobacter baumannii (A. baumannii) is a leading cause of nosocomial infections as this pathogen has certain attributes that facilitate the subversion of natural defenses of the human body. A. baumannii acquires antibiotic resistance determinants easily and can thrive on both biotic and abiotic surfaces. Different resistance mechanisms or determinants, both transmissible and non-transmissible, have aided in this victory over antibiotics. In addition, the propensity to form biofilms (communities of organism attached to a surface) allows the organism to persist in hospitals on various medical surfaces (cardiac valves, artificial joints, catheters, endotracheal tubes, and ventilators) and also evade antibiotics simply by shielding the bacteria and increasing its ability to acquire foreign genetic material through lateral gene transfer. The biofilm formation rate in A. baumannii is higher than in other species. Recent research has shown how A. baumannii biofilm-forming capacity exerts its effect on resistance phenotypes, development of resistome, and dissemination of resistance genes within biofilms by conjugation or transformation, thereby making biofilm a hotspot for genetic exchange. Various genes control the formation of A. baumannii biofilms and a beneficial relationship between biofilm formation and “antimicrobial resistance” (AMR) exists in the organism. This review discusses these various attributes of the organism that act independently or synergistically to cause hospital infections. Evolution of AMR in A. baumannii, resistance mechanisms including both transmissible (hydrolyzing enzymes) and non-transmissible (efflux pumps and chromosomal mutations) are presented. Intrinsic factors [biofilm-associated protein, outer membrane protein A, chaperon-usher pilus, iron uptake mechanism, poly-β-(1, 6)-N-acetyl glucosamine, BfmS/BfmR two-component system, PER-1, quorum sensing] involved in biofilm production, extrinsic factors (surface property, growth temperature, growth medium) associated with the process, the impact of biofilms on high antimicrobial tolerance and regulation of the process, gene transfer within the biofilm, are elaborated. The infections associated with colonization of A. baumannii on medical devices are discussed. Each important device-related infection is dealt with and both adult and pediatric studies are separately mentioned. Furthermore, the strategies of preventing A. baumannii biofilms with antibiotic combinations, quorum sensing quenchers, natural products, efflux pump inhibitors, antimicrobial peptides, nanoparticles, and phage therapy are enumerated.

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“…Second, the phage displayed exogenous peptides or chemical modifications, which could combine with inorganic nanomaterials/drugs, to form phage-nanocomplexes and drugloaded phages. It is well utilized in photodynamic cancer therapy (62)(63)(64). Some researchers used the Fd phage to display a cancertargeting peptide on pVIII major coat protein, and then conjugated photosensitizer at the N-terminal end of the targeting peptides, and demonstrated that the complex of phage-photosensitizers was able to selectively target and kill SKBR3 tumor cells in vitro (65).…”

Section: Ff Phage a Targeted Therapy Vectormentioning

confidence: 99%

Filamentous Bacteriophage—A Powerful Carrier for Glioma Therapy

et al. 2021

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Glioma is a life-threatening malignant tumor. Resistance to traditional treatments and tumor recurrence present major challenges in treating and managing this disease, consequently, new therapeutic strategies must be developed. Crossing the blood-brain barrier (BBB) is another challenge for most drug vectors and therapy medications. Filamentous bacteriophage can enter the brain across the BBB. Compared to traditional drug vectors, phage-based drugs offer thermodynamic stability, biocompatibility, homogeneity, high carrying capacity, self-assembly, scalability, and low toxicity. Tumor-targeting peptides from phage library and phages displaying targeting peptides are ideal drug delivery agents. This review summarized recent studies on phage-based glioma therapy and shed light on the developing therapeutics phage in the personalized treatment of glioma.

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A photo-sensitizable phage for multidrug-resistant Acinetobacter baumannii therapy and biofilm ablation

Abstract: APNB for multidrug-resistant A. Baumannii therapy and biofilms ablation.

Cited by 51 publications

References 41 publications

Multiclawed SiO₂ Nano‐Antibacterial Agent Based on Charge Inversed Ce6 Ionic Liquid Polymers for Combating Oral Biofilm Infection

Multiclawed SiO₂ Nano‐Antibacterial Agent Based on Charge Inversed Ce6 Ionic Liquid Polymers for Combating Oral Biofilm Infection

Convergence of Biofilm Formation and Antibiotic Resistance in Acinetobacter baumannii Infection

Filamentous Bacteriophage—A Powerful Carrier for Glioma Therapy

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A photo-sensitizable phage for multidrug-resistant Acinetobacter baumannii therapy and biofilm ablation

Abstract: APNB for multidrug-resistant A. Baumannii therapy and biofilms ablation.

Cited by 51 publications

References 41 publications

Multiclawed SiO2 Nano‐Antibacterial Agent Based on Charge Inversed Ce6 Ionic Liquid Polymers for Combating Oral Biofilm Infection

Multiclawed SiO2 Nano‐Antibacterial Agent Based on Charge Inversed Ce6 Ionic Liquid Polymers for Combating Oral Biofilm Infection

Convergence of Biofilm Formation and Antibiotic Resistance in Acinetobacter baumannii Infection

Filamentous Bacteriophage—A Powerful Carrier for Glioma Therapy

Contact Info

Product

Resources

About

Multiclawed SiO₂ Nano‐Antibacterial Agent Based on Charge Inversed Ce6 Ionic Liquid Polymers for Combating Oral Biofilm Infection

Multiclawed SiO₂ Nano‐Antibacterial Agent Based on Charge Inversed Ce6 Ionic Liquid Polymers for Combating Oral Biofilm Infection