A phosphofructokinase B-type carbohydrate kinase family protein, PFKB1, is essential for chloroplast development at early seedling stage in rice

Zhu, Xiaobo; Mu, Ze; Yin, Junjie; Chern, Mawsheng; Wang, Mingrui; Zhang, Xiang; Deng, Rui; Li, Yongzhen; Liao, Haicheng; Wang, Long; Tu, Bin; Song, Li; He, Min; Li, Shigui; Wang, Wenming; Chen, Xuewei; Wang, Jing; Li, Weitao

doi:10.1016/j.plantsci.2019.110295

Cited by 9 publications

(7 citation statements)

References 49 publications

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“…To analyze the fructokinase family genes, we identified 15 candidate genes coding pfkB domain proteins in the rice genome using HMMER 3.1 (Table S2). Among them, eight genes (Os01g0894300, Os08g0113100, Os01g0851000, Os03g0602600, Os03g0737701, Os05g0185700, Os01g0105900, Os10g0573000) had been reported as OsFRK1 , OsFRK2 , OsFLN1 , OsFLN2 , OsMIK , OsPUKI , OsDNL-4, and OsPFKB1 , respectively. Interestingly, we found that Os06g0232200 was close to OsFRK2 and shared the same conserved motif with OsFRK1 and OsFRK2 (Figures a and S1).…”

Section: Resultsmentioning

confidence: 99%

New Fructokinase, OsFRK3, Regulates Starch Accumulation and Grain Filling in Rice

Zhang

Tan

Chen

et al. 2023

J. Agric. Food Chem.

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Plant fructokinase (FRK) guarantees the growth and development of higher plants by participating in carbohydrate metabolism. In this study, a new fructokinase, OsFRK3, was identified using bioinformatics analysis, enzyme assay, bacterial growth assay, and yeast complementation test. Then, we created OsFRK3 knockout transgenic lines (osfrk3-1 and osfrk3-2) by the CRISPR/Cas9 technology. We found that the 1000-grain weight decreased notably (approximately −3.6% and −6.1%, respectively) in osfrk3-1 and osfrk3-2. Evidently decreased grain width, grain thickness, and endosperm filling rate were detected in the osfrk3 mutants (osfrk3-1 and osfrk3-2) compared with those of the WT. In addition, the content of seed total starch was significantly decreased by 3.42 and 4.80% in osfrk3 lines, compared with that in the WT. The level of maltose was significantly reduced in the mutants, while that of sucrose and fructose was obviously increased in the mutants. The transcript levels of OsGBSS1, OsBEIIb, OsPFP1β, and OsAGPL1 were significantly decreased in the osfrk3 mutants. These results suggest that OsFRK3 may positively regulate the accumulation of starch through influencing the sugar metabolism.

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Section: Resultsmentioning

confidence: 99%

New Fructokinase, OsFRK3, Regulates Starch Accumulation and Grain Filling in Rice

Zhang

Tan

Chen

et al. 2023

J. Agric. Food Chem.

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“…The high number of downregulated genes associated with photosynthesis may also indicate that oxidative stress was higher in DSL than in DTL. Fructose-1,6bisphosphatase (FBA) is an important metabolic enzyme involved in glycolysis, gluconeogenesis, and the Calvin cycle [38], and 6-phosphofructokinase 1 is a novel regulator indispensable for early chloroplast development [39]. Overexpression of FBA1 can alter growth, photosynthesis, and stress responses in higher plants [40,41].…”

Section: Genes Involved In Photosynthesis and Carbon Metabolismmentioning

confidence: 99%

Comparative analysis of alfalfa (Medicago sativa L.) seedling transcriptomes reveals genotype-specific drought tolerance mechanisms

Wang

et al. 2021

Plant Physiology and Biochemistry

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BackgroundDrought is one of the main abiotic factors that affect alfalfa yield. The identi cation of genes that control this complex trait can provide important insights for alfalfa breeding. However, little is known about how alfalfa responds and adapts to drought stress, particularly in cultivars of differing drought tolerance. ResultsIn this study, the drought-tolerant cultivar Dryland 'DT' and the drought-sensitive cultivar WL343HQ 'DS' were used to characterize leaf and root physiological responses and transcriptional changes in response to water de cit. Under drought stress, Dryland roots (DTR) showed more differentially expressed genes than WL343HQ roots (DSR), whereas WL343HQ leaves (DSL) showed more differentially expressed genes than Dryland leaves (DTL). Many of these genes were involved in stress-related pathways, carbohydrate metabolism, and lignin and wax biosynthesis, which may have improved the drought tolerance of alfalfa. We also observed that several genes related to ABA metabolism, root elongation, peroxidase activity, cell membrane stability, ubiquitination, and genetic processing responded to drought stress in alfalfa. We highlighted several candidate genes, including sucrose synthase, xylan 1,4-betaxylosidase, primary-amine oxidase, and alcohol-forming fatty acyl-CoA reductase, for future studies on drought stress resistance in alfalfa and other plant species. ConclusionsIn summary, our results reveal the unique drought adaptation and resistance characteristics of two alfalfa genotypes. These ndings, which may be valuable for drought resistance breeding, warrant further gene functional analysis to augment currently available information and to clarify the drought stress regulatory mechanisms of alfalfa and other plants. HighlightsWe assembled leaf and root transcriptomes of drought-tolerant and drought-sensitive alfalfa cultivars under control and drought conditions.Under drought, the drought-tolerant cultivar exhibited more differentially expressed metabolites than the drought-sensitive cultivar.Genes associated with carbon metabolism, amino acid metabolism, hormones, and secondary metabolites were differentially expressed under drought stress.The drought-tolerant cultivar exhibited higher expression of genes encoding sucrose synthase, xylan 1,4-beta-xylosidase, primary-amine oxidase, and alcohol-forming fatty acyl-CoA.

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“…It is possible that another protein replaces TCD7 to carry out its function past the fourth leaf, which would explain the normal chloroplast development beyond this stage. The TCD7 paralog PFKB1 (Zhu et al, 2020) might ful ll such a role.…”

Section: Tcd7 Acts During the Second Step Of Chloroplast Developmentmentioning

confidence: 99%

“…By contrast, the rice tcd7 (this study) and wlp2 mutants (Lv et al 2017) had an albino phenotype only at speci c temperatures, raising the possibility that TCD7 and WLP2 expression might respond to temperature. Notably, the expression of PFKB1, a TCD7 paralog, regulates the expression of PEP-encoding genes irrespective of the temperature and may therefore compensate for the loss of TCD7 when its expression subsides (Zhu et al 2020).…”

Section: The Rice Trx-fln Regulatory Module Mediates Pep Activity and Chloroplast Development Under Cold Stressmentioning

confidence: 99%

The Component of the TAC Complex, TCD7, Controls Rice Chloroplast Development at the Early Seedling Stage under Cold Stress

Lin

Kang

Zhou

et al. 2021

Preprint

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Transcriptionally active chromosome (TAC) is a component of protein-DNA complexes with RNA polymerase activity found in chloroplasts. Although TAC in Arabidopsis thaliana has been extensively investigated, how the rice (Oryza sativa L.) TAC complex functions remains largely unknown. We report the characterization of the mutant thermosensitive chlorophyll-deficient7 (tcd7) and the cloning of TCD7. tcd7 mutant seedlings displayed an albino phenotype specifically at low temperatures and before the four-leaf stage. We identified TCD7 by map-based cloning followed by transgenic rescue and genome editing tests, showing that TCD7 encodes the putative TAC component FRUCTOKINASE-LIKE 2 (OsFLN2). TCD7 transcripts were highly abundant in green tissues, and the protein localized to chloroplasts. In agreement with the albino phenotype, transcript levels of genes controlling chloroplast development and the establishment of photosynthetic capacity were severely reduced in tcd7 seedlings at low temperatures, but were expressed as in the wild type at high temperatures, implying that TCD7 regulates the PEP pathway and chloroplast development. Moreover, TCD7 interacted with the thioredoxin OsTRXz to form an OsTRXz-TCD7 regulatory module, which might regulate plastid transcription under cold stress. Our results demonstrate that the nucleus-encoded TAC protein TCD7 protects chloroplast development from cold stress via a TRXz-FLN regulatory module.

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A phosphofructokinase B-type carbohydrate kinase family protein, PFKB1, is essential for chloroplast development at early seedling stage in rice

Cited by 9 publications

References 49 publications

New Fructokinase, OsFRK3, Regulates Starch Accumulation and Grain Filling in Rice

New Fructokinase, OsFRK3, Regulates Starch Accumulation and Grain Filling in Rice

Comparative analysis of alfalfa (Medicago sativa L.) seedling transcriptomes reveals genotype-specific drought tolerance mechanisms

The Component of the TAC Complex, TCD7, Controls Rice Chloroplast Development at the Early Seedling Stage under Cold Stress

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