A perspective profile of ADCY1 in cAMP signaling with drug-resistance in lung cancer

Zou, Ting; Liu, Junyan; She, Li; Chen, Juan; Zhu, Tao; Yin, Ji‐Ye; Li, Xi; Li, Xiangping; Zhou, Hong‐Hao; Liu, Zhao‐Qian

doi:10.7150/jca.36614

Cited by 27 publications

(26 citation statements)

References 84 publications

(80 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further studies are needed to determine whether CRTC3 induces higher expression of adipogenic genes in vivo . In addition, CRTC3 regulates the expression of ADCY family that affects the synthesis of cAMP from ATP and links the regulation of adipose tissue development to adiposity [ 43 , 44 ]. In addition, CRTC3 overexpression also increased the expression of FA metabolism-related genes including the ACOX1 , FADS1/2 and ELOVL3/6 .…”

Section: Discussionmentioning

confidence: 99%

Comprehensive evaluation of the metabolic effects of porcine CRTC3 overexpression on subcutaneous adipocytes with metabolomic and transcriptomic analyses

Liu

Chen

et al. 2021

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Background Meat quality is largely driven by fat deposition, which is regulated by several genes and signaling pathways. The cyclic adenosine monophosphate (cAMP) -regulated transcriptional coactivator 3 (CRTC3) is a coactivator of cAMP response element binding protein (CREB) that mediates the function of protein kinase A (PKA) signaling pathway and is involved in various biological processes including lipid and energy metabolism. However, the effects of CRTC3 on the metabolome and transcriptome of porcine subcutaneous adipocytes have not been studied yet. Here, we tested whether porcine CRTC3 expression would be related to fat deposition in Heigai pigs (a local fatty breed in China) and Duroc×Landrace×Yorkshire (DLY, a lean breed) pigs in vivo. The effects of adenovirus-induced CRTC3 overexpression on the metabolomic and transcriptomic profiles of subcutaneous adipocytes were also determined in vitro by performing mass spectrometry-based metabolomics combined with RNA sequencing (RNA-seq). Results Porcine CRTC3 expression is associated with fat deposition in vivo. In addition, CRTC3 overexpression increased lipid accumulation and the expression of mature adipocyte-related genes in cultured porcine subcutaneous adipocytes. According to the metabolomic analysis, CRTC3 overexpression induced significant changes in adipocyte lipid, amino acid and nucleotide metabolites in vitro. The RNA-seq analysis suggested that CRTC3 overexpression alters the expression of genes and pathways involved in adipogenesis, fatty acid metabolism and glycerophospholipid metabolism in vitro. Conclusions We identified significant alterations in the metabolite composition and the expression of genes and pathways involved in lipid metabolism in CRTC3-overexpressing adipocytes. Our results suggest that CRTC3 might play an important regulatory role in lipid metabolism and thus affects lipid accumulation in porcine subcutaneous adipocytes.

show abstract

Section: Discussionmentioning

confidence: 99%

Comprehensive evaluation of the metabolic effects of porcine CRTC3 overexpression on subcutaneous adipocytes with metabolomic and transcriptomic analyses

Liu

Chen

et al. 2021

J Animal Sci Biotechnol

View full text Add to dashboard Cite

show abstract

“…In addition, it was found that cAMP signaling plays an important role in tumor progression and therapy. [27][28][29][30][31] Therefore, it is speculated that PDE7B may play a role in tumorigenesis through regulating the cAMP levels. Indeed, a number of studies have shown that PDE7B is deregulated in various cancers.…”

Section: Discussionmentioning

confidence: 99%

<p>Identification of <em>PDE7B</em> as a Potential Core Gene Involved in the Metastasis of Clear Cell Renal Cell Carcinoma</p>

Sun¹,

Zou²,

Ouyang³

et al. 2020

CMAR

View full text Add to dashboard Cite

Background: Metastasis is the main cause of treatment failure in various cancer, including ccRCC. However, the key genes involved in ccRCC metastasis remain largely unknown. Purpose: The identification of the aberrant gene expression patterns associated with metastatic traits is of great clinical significance. The aim of this study was to investigate the clinical significance and function of PDE7B in ccRCC. Materials and Methods: Expression profiling data for patient-matched primary and metastatic ccRCC tumors were obtained from GEO Dataset. Limma package was used to identify differentially expressed genes (DEGs) between the metastatic and the primary groups. Gene Ontology, Kyoto Encyclopedia of Genes Genomes (KEGG), and PPI network analysis were used to study the interacting activities and the interconnection of the DEGs. CCK-8 assays and Transwell assays were performed to detect the proliferation and migration of renal cancer cells. Results: We obtained 163 DEGs, including 132 that were upregulated and 31 that were downregulated in metastatic ccRCC tissues. Both Gene Ontology function and KEGG pathway analysis showed that DEGs were involved in extracellular matrix (ECM) organization and cell adhesion. After utilizing PPI network to explore the interconnection among the DEGs, 22 genes were selected as the hub genes. Subsequently, survival analysis revealed that seven hub genes (SFN, NKX2-1, HP, MAPT, EPHA4, KCNAB1, and PDE7B) were significantly associated with overall survival disease-specific survival, and progression-free interval in ccRCC. Moreover, the low expression of PDE7B was found in clinical ccRCC samples and correlated with TNM stage and histologic grade. We further showed that knockdown of PDE7B increased cell growth and migration of renal cancer cells. Conclusion: Our results implicated that PDE7B may play a key role in the development of metastatic RCC.

show abstract

“…In addition, the DEG PRL was identified, which was enriched in the most pathways related to hypothalamic function and follicular development, including the neuroactive ligand–receptor interaction, PI3K–Akt, and prolactin signaling pathways. PRL is demonstrated to be expressed in the hypothalamus ( Roselli et al, 2008 ; Zou et al, 2019 ). That is, hypothalamic PRL mainly functions in the transmission of neural signals and the synthesis and secretion of reproductive hormones.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Differentially Expressed Genes and Long Non-coding RNAs Associated With Fecundity in Sheep Hypothalamus With Different FecB Genotypes

Chen

Guo

et al. 2021

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Small-tailed Han sheep, with different FecB genotypes, manifest distinct ovulation rates and fecundities, which are due to differences in reproductive hormones secreted by the hypothalamic–pituitary–ovarian axis. Nevertheless, the function of the hypothalamus against a FecB mutant background on increasing ovulation rate is rarely reported. Therefore, we determined the expression profiles of hypothalamus tissue collected from six wild-type (WW) and six FecB mutant homozygous (BB) ewes at the follicular and luteal phases by whole-transcriptome sequencing. We identified 53 differentially expressed mRNAs (DEGs) and 40 differentially expressed long non-coding RNAs (DELs) between the two estrus states. Functional annotation analysis revealed that one of the DEGs, PRL, was particularly enriched in the hypothalamic function, hormone-related, and reproductive pathways. The lncRNA–target gene interaction networks and KEGG analysis in combination suggest that the lncRNAs LINC-676 and WNT3-AS cis-acting on DRD2 and WNT9B in different phases may induce gonadotropin-releasing hormone (GnRH) secretion. Furthermore, there were differences of regulatory elements and WNT gene family members involved in the follicular–luteal transition in the reproductive process between wild-type (WNT7A) and FecB mutant sheep (WNT9B). We combined the DEG and DEL data sets screened from different estrus states and genotypes. The overlap of these two sets was identified to select the mRNAs and lncRNAs that have major effects on ovulation. Among the overlapping molecules, seven DEGs and four DELs were involved in the follicular–luteal transition regulated by FecB mutation. Functional annotation analysis showed that two DEGs (FKBP5 and KITLG) were enriched in melanogenesis, oxytocin, and GnRH secretion. LINC-219386 and IGF2-AS were highly expressed in the BB ewes compared with WW ewes, modulating their target genes (DMXL2 and IGF2) to produce more GnRH during follicular development, which explains why mutated ewes produced more mature follicles. These results from expression profiling of the hypothalamus with the FecB mutation at different estrus states provide new insights into how the hypothalamus regulates ovulation under the effect of the FecB mutation.

show abstract

A perspective profile of ADCY1 in cAMP signaling with drug-resistance in lung cancer

Cited by 27 publications

References 84 publications

Comprehensive evaluation of the metabolic effects of porcine CRTC3 overexpression on subcutaneous adipocytes with metabolomic and transcriptomic analyses

Comprehensive evaluation of the metabolic effects of porcine CRTC3 overexpression on subcutaneous adipocytes with metabolomic and transcriptomic analyses

<p>Identification of <em>PDE7B</em> as a Potential Core Gene Involved in the Metastasis of Clear Cell Renal Cell Carcinoma</p>

Transcriptome Analysis Reveals Differentially Expressed Genes and Long Non-coding RNAs Associated With Fecundity in Sheep Hypothalamus With Different FecB Genotypes

Contact Info

Product

Resources

About