A perspective on 3D bioprinting in tissue regeneration

Ahadian, Samad; Khademhosseini, Ali

doi:10.1007/s42242-018-0020-3

Cited by 68 publications

(36 citation statements)

References 17 publications

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“…Reprinted from Zhang et al (2019), Copyright 2019, with permission from Elsevier the outer shell portion of the structure with a certain thickness is constructed using the extrusion printing module. Accurate geometric characteristic construction and multi-material combinations are the main advantages of 3D bioprinting technology (Ahadian and Khademhosseini, 2018). Using this method, it is feasible to manufacture geometry-controllable biosynthetic corneas with controllable thickness and curvature.…”

Section: Fig 1 Schematic Of Corneal Substitute Developmentmentioning

confidence: 99%

Integrated 3D bioprinting-based geometry-control strategy for fabricating corneal substitutes

Zhang

Xue

et al. 2019

J. Zhejiang Univ. Sci. B

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Background: The shortage of donor corneas is a severe global issue, and hence the development of corneal alternatives is imperative and urgent. Although attempts to produce artificial cornea substitutes by tissue engineering have made some positive progress, many problems remain that hamper their clinical application worldwide. For example, the curvature of tissue-engineered cornea substitutes cannot be designed to fit the bulbus oculi of patients. Objective: To overcome these limitations, in this paper, we present a novel integrated three-dimensional (3D) bioprintingbased cornea substitute fabrication strategy to realize design, customized fabrication, and evaluation of multi-layer hollow structures with complicated surfaces. Methods: The key rationale for this method is to combine digital light processing (DLP) and extrusion bioprinting into an integrated 3D cornea bioprinting system. A designable and personalized corneal substitute was designed based on mathematical modelling and a computer tomography scan of a natural cornea. The printed corneal substitute was evaluated based on biomechanical analysis, weight, structural integrity, and fit. Results: The results revealed that the fabrication of high water content and highly transparent curved films with geometric features designed according to the natural human cornea can be achieved using a rapid, simple, and low-cost manufacturing process with a high repetition rate and quality. Conclusions: This study demonstrated the feasibility of customized design, analysis, and fabrication of a corneal substitute. The programmability of this method opens up the possibility of producing substitutes for other cornea-like shell structures with different scale and geometry features, such as the glomerulus, atrium, and oophoron.

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Section: Fig 1 Schematic Of Corneal Substitute Developmentmentioning

confidence: 99%

Integrated 3D bioprinting-based geometry-control strategy for fabricating corneal substitutes

Zhang

Xue

et al. 2019

J. Zhejiang Univ. Sci. B

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“…Three-dimensional (3D) bioprinting has emerged as a powerful microscale technology for tissue biofabrication, offering the ability to customize shape, material, and structure of tissue scaffolds [10,11]. Bioprinting has also allowed for the incorporation of cells and soluble factors during printing process [12].…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Bioprinting of Functional Skeletal Muscle Tissue Using Gelatin Methacryloyl-Alginate Bioinks

et al. 2019

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Skeletal muscle tissue engineering aims to fabricate tissue constructs to replace or restore diseased or injured skeletal muscle tissues in the body. Several biomaterials and microscale technologies have been used in muscle tissue engineering. However, it is still challenging to mimic the function and structure of the native muscle tissues. Three-dimensional (3D) bioprinting is a powerful tool to mimic the hierarchical structure of native tissues. Here, 3D bioprinting was used to fabricate tissue constructs using gelatin methacryloyl (GelMA)-alginate bioinks. Mechanical and rheological properties of GelMA-alginate hydrogels were characterized. C2C12 myoblasts at the density 8 × 106 cells/mL were used as the cell model. The effects of alginate concentration (0, 6, and 8% (w/v)) and crosslinking mechanism (UV crosslinking or ionic crosslinking with UV crosslinking) on printability, cell viability, proliferation, and differentiation of bioinks were studied. The results showed that 10% (w/v) GelMA-8% (w/v) alginate crosslinked using UV light and 0.1 M CaCl2 provided the optimum niche to induce muscle tissue formation compared to other hydrogel compositions. Furthermore, metabolic activity of cells in GelMA bioinks was improved by addition of oxygen-generating particles to the bioinks. It is hoped that such bioprinted muscle tissues may find wide applications in drug screening and tissue regeneration.

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“…However, alginate impedes the functionalization of encapsulated cells, as it is not synthetized from natural mammal materials. Gelatin methacryloyl (GelMA), which is synthetized from gelatin, has become more and more attractive owing to its remarkable biocompatibility for cellular functionalization and rapid crosslinking ability, which has led to its widespread application in mini‐tissue rebuilding, organ‐on‐a‐chip, and drug controlled release . A low concentration of GelMA has been reported to be more appropriate for cellular functionalization and growth, as it forms larger pores, while showing superior elasticity and an enhanced degradation profile.…”

Section: Introductionmentioning

confidence: 99%

Electro‐Assisted Bioprinting of Low‐Concentration GelMA Microdroplets

Xie

Gao

Zhao

et al. 2018

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Low‐concentration gelatin methacryloyl (GelMA) has excellent biocompatibility to cell‐laden structures. However, it is still a big challenge to stably fabricate organoids (even microdroplets) using this material due to its extremely low viscosity. Here, a promising electro‐assisted bioprinting method is developed, which can print low‐concentration pure GelMA microdroplets with low cost, low cell damage, and high efficiency. With the help of electrostatic attraction, uniform GelMA microdroplets measuring about 100 μm are rapidly printed. Due to the application of lower external forces to separate the droplets, cell damage during printing is negligible, which often happens in piezoelectric or thermal inkjet bioprinting. Different printing states and effects of printing parameters (voltages, gas pressure, nozzle size, etc.) on microdroplet diameter are also investigated. The fundamental properties of low‐concentration GelMA microspheres are subsequently studied. The results show that the printed microspheres with 5% w/v GelMA can provide a suitable microenvironment for laden bone marrow stem cells. Finally, it is demonstrated that the printed microdroplets can be used in building microspheroidal organoids, in drug controlled release, and in 3D bioprinting as biobricks. This method shows great potential use in cell therapy, drug delivery, and organoid building.

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A perspective on 3D bioprinting in tissue regeneration

Cited by 68 publications

References 17 publications

Integrated 3D bioprinting-based geometry-control strategy for fabricating corneal substitutes

Integrated 3D bioprinting-based geometry-control strategy for fabricating corneal substitutes

Three-Dimensional Bioprinting of Functional Skeletal Muscle Tissue Using Gelatin Methacryloyl-Alginate Bioinks

Electro‐Assisted Bioprinting of Low‐Concentration GelMA Microdroplets

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