A peroxidase-coupled method for the colorimetric determination of serum triglycerides.

McGowan, M.; Artiss, Joseph D.; Strandbergh, Donald R.; Zak, Bennie

doi:10.1093/clinchem/29.3.538

Cited by 1,067 publications

(358 citation statements)

References 10 publications

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“…Total cholesterol (T-chol) was analysed by a cholesterol oxidase method (Boerhinger-Mannheim, Mannheim, Germany) and triacylglyceride (Tag) by a lipase-glycerol kinase method (Sigma, Poole, Dorset, UK) with no free glycerol correction [8,9]. Both analyses were carried out on a Cobas Fara analyser (Roche Diagnostic Systems, Basel).…”

Section: Methodsmentioning

confidence: 99%

Glycaemic control and familial factors determine hyperlipidaemia in early childhood diabetes

et al. 1999

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Section: Methodsmentioning

confidence: 99%

Glycaemic control and familial factors determine hyperlipidaemia in early childhood diabetes

et al. 1999

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“…HDLcholesterol was measured with the same method after precipitation of very low-density and low-density lipoprotein with phosphotungstic acid and magnesium ions [18]. TG was assayed using an enzymatic method [19]. HbA 1c was measured using an automated HPLC analyser (Diamat BioRed Laboratories, NY, USA).…”

Section: Methodsmentioning

confidence: 99%

Can reduction in hypertriglyceridaemia slow progression of microalbuminuria in patients with non‐insulin‐dependent diabetes mellitus?

Smulders

Eeden

Stehouwer

et al. 1997

Eur J Clin Investigation

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The objective of this study was to investigate whether reduction in hypertriglyceridaemia is associated with a slower rate of progression of microalbuminuria in patients with non-insulin-dependent diabetes mellitus (NIDDM). Fifteen normotensive NIDDM patients with hypertriglyceridaemia (> 2.5 mmol L-1) and microalbuminuria were randomly selected to receive either placebo (eight patients) or gemfibrozil 600 mg b.i.d. (seven patients). Progression of microalbuminuria was assessed during a 12-month follow-up period with measurements, consisting of blood tests and triplicate 24-h urine collections, at 1, 3, 6, 9 and 12 months. All but one patient in the treatment group showed a favourable response (> or = 20% reduction) of hypertriglyceridaemia to gemfibrozil. One patient in the placebo group showed a spontaneous reduction in triglyceride levels. Progression of microalbuminuria was lower, although not statistically significantly so, in the treatment group (36%) than in the placebo group (65%). In the group with > or = 20% reduction in triglyceride levels, progression of MA was significantly lower than in the group with stable or increasing triglyceride levels (+1%, range -56% to +49% vs. +97%, range -35% to +202% respectively) (P = 0.03). Continued follow-up data of patients switching from placebo to gemfibrozil after the trial further support the role of serum triglyceride reduction in stabilizing albumin excretion. In conclusion, the results indicate that, in microalbuminuric NIDDM patients, effective treatment of dyslipidaemia could be associated with stabilization of urinary albumin excretion.

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“…Triglyceride content of a sample was determined using the triglycerides lipase/peroxidase method (Fossati & Prencipe 1982;McGowan et al 1983) closely following the protocol provided by the Infinity Triglyceride kit of Thermo Electron. Two point five microlitre of supernatant was added to 125 lL of triglyceride reagent, and the absorbance measured at 520 nm after 10 min of incubation at room temperature in the dark.…”

Section: Quantification Of Storage Moleculesmentioning

confidence: 99%

Alternative growth and energy storage responses to mortality threats in damselflies

2005

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The role of physiology in mediating the growth/predation risk trade-off has been largely ignored. We examined effects of predation risk on relationships between growth and storage molecules in Enallagma aspersum and Ischnura verticalis damselfly larvae that differ in this trade-off. In laboratory and field experiments, both species had similar growth and mortality rates and similar concentrations of storage molecules in the absence of mortality threats. However, in the presence of dragonfly predators Ischnura larvae had higher mortality rates and grew faster than Enallagma larvae. Consistent with the difference in growth rate, EnallagmaÕs total protein concentrations decreased under predation risk while those of Ischnura did not. Glucose and glycogen concentrations were not affected, while triglyceride concentrations were lower under predation risk in Enallagma but not in Ischnura. Species differences at the physiological level to the presence of mortality threats may be crucial to understanding patterns in metamorphic and post-metamorphic traits.

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A peroxidase-coupled method for the colorimetric determination of serum triglycerides.

Cited by 1,067 publications

References 10 publications

Glycaemic control and familial factors determine hyperlipidaemia in early childhood diabetes

Glycaemic control and familial factors determine hyperlipidaemia in early childhood diabetes

Can reduction in hypertriglyceridaemia slow progression of microalbuminuria in patients with non‐insulin‐dependent diabetes mellitus?

Alternative growth and energy storage responses to mortality threats in damselflies

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