Abstract:Dehaloperoxidase (DHP) catalyzes detoxifying halophenols. It is a heme-containing enzyme using H 2 O 2 as the oxidant. Heme bleaching from the active site is of great concern. In addition, the interference of DHP by H 2 O 2 leads to the inactivation of the enzyme. To solve these two problems, DHP is coordinated to Zn (II) in PBS buffer to form a biomineralized composite (DHP&Zn-CP). DHP&Zn-CP was characterized by measuring SEM and confocal images, as well as energy dispersive X-ray spectrometry mapping. Fluore… Show more
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