A Permanent Rat Gastric Fistula

Abstract

1have clinical significance which is being evaluated currently. Other efforts are being directed toward isolation and identification of the diffusible substance and the plaque material which it produces and an elucidation of the specific substrates involved.The diffusible substance that activates production of the red band differs from the substance that produces the plaque material. This is demonstrated by the fact that whereas all the bloods used for preparation of the media were able to act as substrate for the red band, a few did not act as substrate for production of plaques.Summary. Macroscopically visible lipid plaques were produced on the surface of human blood agar plates and on 10% plasma agar plates when they were inoculated centrally with certain strains of coagulase-positive S. aureus or exposed to active sterile agar segments ( ASA) . Similar lipid plaques covered the surface of liquid media containing plasma when activated sterile agar segments were suspended in the media. Staphylococ-cus antitoxin prevented the appearance of the lipid plaques. The lipid plaques consist primarily of octadecenoic acid, both free and in triglycerides. Lesser amounts of stearic, palmitic and various shorter chain acids are also present. The most likely explanation for the phenomenon is that a lipase released by certain strains of staphylococci acts upon the lipids in human plasma.

Influence of Dietary Fat on Histamine Stimulated Gastric Secretion in Rats

“…Surgery was performed under ether anesthesia. Chronic gastric fistula was produced in each animal, and a miniature stainless steel cannula [7] implanted in the anterior wall of the rumen. A plaque of broad mesh nylon was applied on the cannula and positioned, during the operation, between the exterior wall of the stomach and the interior wall of the abdomen.…”

Доктор Медицины Латвийского Университета Семен Комаров (1892-1964)

“…Наконец, париетальная клеточная масса не уменьшается после адреналэктомии, несмотря на спад секреции. Работы С. Комарова с крысами имеет большую ценность для нашего знания желудочной физиологии и патологии, а техника, описанная им, стала широко известной и получила признание [46,49,51,52,54,69,93,[95][96][97][98][99][100][101][102][103][104][105][106][107][108][109][110][111]113].…”

Bioassay of Inhibitory Activity of Gastrone on Basal HCl Secretion in Rats with Chronic Gastric Fistula

“…This technique, introduced by Komarov et al (26) and improved by other investigators (6, 7) has been successfully used for the study of the effects of various stimulants, inhibitors and drugs on gastric secre tion (27). …”