A PCR‐RFLP Test to Detect Allelic Variants of the Bovine Kappa‐Casein Gene

Abstract

Point mutations in exon IV of bovine kappa-casein gene (kappaCn, CASK, CSN3) determine nine allelic variants (A, B, C, E, F, G, H, I, and A1) for the gene. These variants are associated with major differences in composition and manufacturing properties of milk (i.e., cheese yield). A PCR-RFLP test was developed in order to distinguish the different alleles. Polymorphisms are detected by digestion with the endonucleases HindIII, HaeIII, and MaeII followed by electrophoresis in agarose gels stained with ethidium bromide. Twenty eight DNA samples from different breeds of Argentina were analyzed for the A, B, and E variants. This simple PCR-RFLP test makes feasible the inclusion of kappa-casein genotypes in breeding plans.

Relationship Between Kappa Casein Genes (CSN3) and Industrial Yield in Holstein Cows in Nariño-Colombia

“…In our studied population, only two allelic variants were found (A and B), identifying the homozygous genotypes AA, BB and heterozygous AB, in accordance with the electrophoretic pattern described by [12] ( Figure 3). These results are consistent with those reported by other researchers, in which the A and B alleles have the highest frequency for the CSN3 gene in dairy breeds [8,33,50,47]. The least square means for curd yield were compared using the Tukey -Kramer test, concluding that the BB genotype had the highest yield compared to genotypes AA and AB, and no significant differences were found between AA and BB, as shown in Figure 4.…”

“…From 1983 to 2007, 11 allelic variants have been reported for κ-Cs (A, B, C, E, F, G, H, I, A1, A2, A3), but in Bos taurus variants A and B are the most frequent alleles and not all of them appear in all races [33,50,51,47,52,53].…”

Association between CSN3 and BCO2 gene polymorphisms and milk performance traits in the Czech Fleckvieh cattle breed

“…Primers are shown in Table 1, and the length of PCR fragments and annealing temperatures are shown in Table 2. PCR for the CSN3 gene detection was performed according to Soria et al (2003) and for the BCO2 gene according to Tian et al (2010). Amplification of genomic DNA was performed in a Veriti ® 96-Well Thermal Cycler (Applied Biosystems, USA).…”

Kappa-Casein Genotypic Frequencies in Holstein-Friesian Dairy Cattle in West Java Province

“…The genetic polymorphism of the κ-Cn gene in ruminant has been well documented providing ca le (Pinders et al, 1991;Rij njkels et al, 1997), buff aloes (Pipalia et al, 2001;Otaviano et al, 2005;Patel et al, 2007;Riaz et al, 2008), goats (Angiolillo et al, 2002;Yahyaoui et al, 2003;Prinzenberg et al, 2005;Kiplagat et al, 2010), sheep (Moioli et al, 2007) and yak (Prinzenberg et al, 2008). Eleven variant alleles of the κ-Cn gene have been identifi ed in ca le, namely A, B, C, D, E, F, G, H, Al, and J (Soria et al, 2003). However, only two alleles, namely A and B alleles, are the most commonly identifi ed in Bos taurus dairy ca les (Beata et al, 2008).…”