A PCR-microarray method for the screening of genetically modified organisms

Four new SYBR®Green qPCR screening methods for the detection of Roundup Ready®, LibertyLink®, and CryIAb traits in genetically modified products

“…Several multiplex qPCR formats are available reducing the number of analyses and facilitating high throughput but requiring multi-channel detection devices and often including costly detection probes for at least some of the targets [8][9][10]. In other cases, the applied chemistries are quite complex or are to be combined with other technologies that are less appropriate for routine applications [11][12][13][14][15][16]. Several of these approaches involve the use of PCR with multiple targets and consecutive detection and identification of the amplification products using microarrays [11][12][13].…”

“…In other cases, the applied chemistries are quite complex or are to be combined with other technologies that are less appropriate for routine applications [11][12][13][14][15][16]. Several of these approaches involve the use of PCR with multiple targets and consecutive detection and identification of the amplification products using microarrays [11][12][13]. Apart from requiring additional costly array analysis equipment, these approaches are often prone to variable quality of the array chips making them less suitable for routine or enforcement purposes [17].…”

Development of a Molecular Platform for GMO Detection in Food and Feed on the Basis of “Combinatory qPCR” Technology

“…At the present time several high-tech strategies like multiplex PCR and consecutive detection and identification of the amplification products using micro-arrays (Chaouachi et al, 2008, Morisset et al, 2008, Hamels et al, 2009 or PCR combined with capillary electrophoresis (Nadal et al, 2009) have been proposed to deal with this discriminative problem and the broad diversity of GMO. However, at the present time, these technologies require additional costly equipment and investments in technical support.…”

“…the cryIAb/Ac) are being used and detection methods have been developed (Bravo et al, 2007). It should however be noted that the above-mentioned methods are mostly either end-point detection on agarose gel or real-time qPCR using TaqMan® chemistry (Hamels et al, 2009;Raymond et al, 2010;Nadal et al, 2009;Prins et al, 2008). Development of screening methods using the SYBR ® Green qPCR technology only started recently Barbau-Piednoir et al, 2011;Mbongolo Mbella et al, 2011) although this approach offers a number of advantages over the TaqMan chemistry.…”

Reliability of PCR based screening for identification and quantification of GMOs

“…For that reason, laboratories call for simple cost effective assays as suggested e.g. by Hamels et al (2009) or Chaouachi et al (2008. However, they need to be approved by practice.…”