A PCR-based test for species-specific determination of heat treatment conditions of animal meals as an effective prophylactic method for bovine spongiform encephalopathy

An overview of tests for animal tissues in feeds applied in response to public health concerns regarding bovine spongiform encephalopathy

“…In the first study, Kingombe et al (55) concluded that on properly rendered material, detection of bovine DNA segments by PCR fails because the DNA is too fragmented. They used two targets (274 bp and 464 bp) and a standard, resin-based DNA extraction protocol.…”

Application of cholesterol determination method to indirectly detect meat and bone meals in ruminant feeds

“…3 Therefore, monitoring of the quality and origin of feed components becomes an instrumental factor in the control of BSE, 6 which in turn can guarantee a totally reliable product for the consumers d. 4,9 Thus far, several methods have been proposed for the detection of animal by-products in ruminant feed. [10][11][12][13][14][15][16][17][18][19] Some are based on immunoassay techniques for the detection of animal protein that present a low detection limit, besides being easy to apply. 12,13 Nevertheless, in most cases, immunoassays present a cross reaction with certain compounds, yielding incorrect results or overestimating the concentrations at the time of reading.…”

“…12,13 Nevertheless, in most cases, immunoassays present a cross reaction with certain compounds, yielding incorrect results or overestimating the concentrations at the time of reading. Other methods, such as the polymerase chain reaction (PCR) for the species-specific identification of DNA have also been reported previously, [14][15][16][17][18] using the enzyme-linked immunosorbent assay (ELISA) [20][21][22] or ELISA plus gas chromatography, to detect tissues from the central nervous system. 23,24 The non-chromatographic techniques present a low detection limit, but lack specificity.…”

DNA based approaches for the detection and identification of bovine meat and bone meal in feeds

“…Several studies have dealt with the application of polymerase chain reaction (PCR) for the detection of bovine tissue in animal feeds (3,27,28,39,41). In many studies (3,5,6,12,14,26,29,30,32), a species-specific PCR has been developed to identify bovine, porcine and avian tissues in meat and bone meal.…”