A pathogenicity locus of Streptococcus gallolyticus subspecies gallolyticus

Abstract

Streptococcus gallolyticus subspecies gallolyticus (Sgg) is known to be strongly associated with colorectal cancer (CRC). Recent functional studies further demonstrated that Sgg actively stimulates CRC cell proliferation and promotes the development of colon tumors. However, the Sgg factors important for the pro-proliferative and pro-tumor activities of Sgg remain unclear. Here, we identified a chromosomal locus in Sgg strain TX20005. Deletion of this locus significantly reduced Sgg adherence to CRC cells and abrogated the ability of Sgg to stimulate CRC cell proliferation. Thus, we designate this locus as the Sgg pathogenicity-associated region (SPAR). More importantly, we found that SPAR is important for Sgg pathogenicity in vivo. In a gut colonization model, mice exposed to the SPAR deletion mutant showed significantly reduced Sgg load in the colonic tissues and fecal materials, suggesting that SPAR contributes to the colonization capacity of Sgg. In a mouse model of CRC, deletion of SPAR abolished the ability of Sgg to promote the development of colon tumors growth. Taken together, these results highlight SPAR as a critical pathogenicity determinant of Sgg.

Sciatica caused by spinal epidural abscess as the initial clinical presentation of colon cancer: a rare case report and review of literature

“…adherence to colorectal cancer cells and abrogated the ability of Sgg. to stimulate cancer cell proliferation [ 22 ]. Taken together, whether Sgg.…”

Characterization of TelE, a T7SS LXG Effector Exhibiting a Conserved C-Terminal Glycine Zipper Motif Required for Toxicity

“…However, the underlying molecular mechanisms are not known. Recently, the same group reported the identification of a 12-gene chromosomal locus named SPAR (for Sgg pathogenicity-associated region) reducing the adherence of TX20005 to the host colonic cells and abrogating its ability to stimulate cell proliferation ( 49 ). The SPAR mutant phenocopies the T7SSb knockout mutant, connecting the SPAR locus to the T7SSb.…”

Current Knowledge of Enterococcal Endocarditis: A Disease Lurking in Plain Sight of Health Providers

“…It is uncertain whether there is a significant association between these clinical conditions, as seen in most cases of Streptococcus gallolyticus subsp. gallolyticus endocarditis, previously associated with Streptococcus bovis biotype I [ 122 , 123 , 124 , 125 , 126 ]. Stanley et al [ 127 ] found that a murine model of ischemic-reperfusion stroke showed bacteraemia caused by a specific group of commensal bacterial strains, with enterococci being the most prevalent.…”