A passive micromixer for enzymatic digestion of DNA

Papadopoulos, Vasileios; Kefala, Ioanna N.; Kaprou, Georgia D.; Kokkoris, George; Moschou, Despina; Papadakis, George; Gizeli, Electra; Tserepi, Angeliki

doi:10.1016/j.mee.2014.04.011

Cited by 46 publications

(30 citation statements)

References 27 publications

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“…5). The height of the channel is 60 μm, equal to the thickness of the photo-imageable dry film (Papadopoulos et al 2014) where the channel will be formed. The minimum width of the channels is 150 μm, appropriate for a reproducible lithographic result.…”

Section: The Proposed Design and Conditions Of The Numerical Evaluationmentioning

confidence: 99%

“…The DuPont™ Pyralux ® PC is routinely used in the FPC industry as a coverlay to protect circuitry. Its implementation as a structural material for microfluidics has been proposed recently by our group (Papadopoulos et al 2014).…”

Section: Micromixer Fabricationmentioning

confidence: 99%

“…The proposed technology allows the integration of microfluidic devices with heterogeneous components: electronic circuits, sensors, and microheaters (Moschou et al 2013) that can be utilized for on-chip detection and control in a cost-effective manner, following the current trend in LoC technology (Aracil et al 2015;Kim et al 2011;Wu et al 2010). To the best of our knowledge, the use of this dry-film resist proposed by our team (Papadopoulos et al 2014) and implemented in this work as structural material of microfluidic devices is very rare in the literature.…”

Section: Introductionmentioning

confidence: 99%

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A labyrinth split and merge micromixer for bioanalytical applications

Kefala

Papadopoulos

Karpou

et al. 2015

Microfluid Nanofluid

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microscopy, while it is further validated for enzymatic digestion of DNA. The latter is achieved even within 30 s of sufficient mixing of DNA and enzyme solutions through the SAM. Despite the numerous works on micromixers, the labyrinth-SAM is a novel design of an efficient passive micromixer. The efficiency together with its simplicity, which is manifested by (a) the planar (and not complex three-dimensional) geometry, (b) the two-inlet, instead of multiple-inlet, configuration, (c) the small number of fabrication steps, and (d) the compatibility with mass production, makes the proposed micromixer a good candidate for integration in bioanalytical miniaturized platforms.

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Section: The Proposed Design and Conditions Of The Numerical Evaluationmentioning

confidence: 99%

Section: Micromixer Fabricationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A labyrinth split and merge micromixer for bioanalytical applications

Kefala

Papadopoulos

Karpou

et al. 2015

Microfluid Nanofluid

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show abstract

“…On the other hand, in continuous flow (fixed-loop or closed-loop) devices [12], the sample moves through fixed temperature zones to achieve the required thermal cycling, leading to faster DNA amplification and lower power consumption. However, the use of thin (flexible) polymeric films for the fabrication of μPCR devices or other heated devices [13,14], and the evolution of the heating elements from external to integrated on chip at a small distance from the DNA sample, allows further reduction in the thermal mass of the devices and as a consequence in rapid heating/cooling rates [15]. Despite these advantages, a shift towards isothermal methods in microfabricated diagnostics is observed mainly stemming from their reduced thermal budget.…”

Section: Introductionmentioning

confidence: 99%

“…A new concept for mutation screening and detection based on acoustic wave sensing was recently shown [16]. The suggested method is suitable for a LOC concept through integration of acoustic devices that are amenable to miniaturization with low-cost microfluidic devices that can be used for DNA amplification and further manipulations such as restriction digestion [14]. Towards the goal of the development of such an integrated platform for mutation detection, we have used SC and CF devices to amplify a DNA fragment of the exon 20 of the BRCA1 gene that contains at least two positions where significant mutations occur [17].…”

Section: Introductionmentioning

confidence: 99%

Miniaturized devices towards an integrated lab-on-a-chip platform for DNA diagnostics

et al. 2015

Self Cite

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Microfluidics is an emerging technology enabling the development of Lab-on-a-chip (LOC) systems for clinical diagnostics, drug discovery and screening, food safety and environmental analysis. LOC systems integrate and scale down one or several laboratory functions on a single chip of a few mm 2 to cm 2 in size, and account for many advantages on biochemical analyses, such as low sample and reagent consumption, low cost, reduced analysis time, portability and point-of-need compatibility. Currently, available nucleic acid diagnostic tests take advantage of Polymerase Chain Reaction (PCR) that allows exponential amplification of portions of nucleic acid sequences that can be used as indicators for the identification of various diseases. Here, we present a comparison between static chamber and continuous flow miniaturized PCR devices, in terms of energy consumption for devices fabricated on the same material stack, with identical sample volume and channel dimensions. The comparison is implemented by a computational study coupling heat transfer in both solid and fluid, mass conservation of species, and joule heating. Based on the conclusions of this study, we develop low-cost and fast DNA amplification devices for both PCR and isothermal amplification, and we implement them in the detection of mutations related to breast cancer. The devices are fabricated by mass production amenable technologies on printed circuit board (PCB) substrates, where copper facilitates the incorporation of on-chip microheaters, defining the thermal zones necessary for PCR or isothermal amplification methods.

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Analysis of a microreactor for synthesizing nanocrystals by computational fluid dynamics

et al. 2018

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Microreactors eliminate batch‐to‐batch variability and allow better control over nanocrystal synthesis. A serpentine microreactor fabricated by femtosecond laser ablation is presented and characterized by computational fluid dynamics, since the micro channels show a trapezoidal cross‐section mainly due to the relatively high numerical aperture of the focusing lens. Mixing, macro and micro, throughout the device was investigated for inlet flow rates between 10–500 μL min−1 and the injection of an inert tracer with the same transport properties of water. The simulation of the whole microreactor enabled the analysis of the formation and destruction of structures. For instance, secondary flows played a major role in mixing behaviour: small flow rates did not promote mixing of the tracer and a stream of pure water even after 43 curved segments, while they were perfectly mixed after 9 segments for higher flow rates. According to the mixing index, the maximum effect of convective mixing was achieved for an inlet flow rate of 250 μL min−1. Tracer dispersion and the mixing index guided a scale‐up process of the microreactor, optimizing the number of curved segments while increasing total throughput. The upscaled design exhibited mixing saturation at 400 μL min−1 and promoted better control of residence time to allow nanocrystal growth.

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A passive micromixer for enzymatic digestion of DNA

Cited by 46 publications

References 27 publications

A labyrinth split and merge micromixer for bioanalytical applications

A labyrinth split and merge micromixer for bioanalytical applications

Miniaturized devices towards an integrated lab-on-a-chip platform for DNA diagnostics

Analysis of a microreactor for synthesizing nanocrystals by computational fluid dynamics

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