A partial proteome reference map of the wine lactic acid bacterium Oenococcus oeni ATCC BAA-1163

Abstract

Oenococcus oeni is the main lactic acid bacterium that carries out the malolactic fermentation in virtually all red wines and in some white and sparkling wines. Oenococcus oeni possesses an array of metabolic activities that can modify the taste and aromatic properties of wine. There is, therefore, industrial interest in the proteins involved in these metabolic pathways and related transport systems of this bacterium. In this work, we report the characterization of the O. oeni ATCC BAA-1163 proteome. Total and membrane protein preparations from O. oeni were standardized and analysed by two-dimensional gel electrophoresis. Using tandem mass spectrometry, we identified 224 different spots corresponding to 152 unique proteins, which have been classified by their putative function and subjected to bioinformatics analysis.

Ethanol stress in Oenococcus oeni : transcriptional response and complex physiological mechanisms

“…; Mohedano et al . ; Napoli et al . ), even if the use of classical 2‐DE for the analysis of proteome is problematic since many proteins resolve poorly in the dimension of the pH gradient.…”

“…The two-dimensional gel electrophoresis (2-DE) technique has provided information on the adaptive response of O.oeni to changes in growth conditions (Silveira et al 2004;Mohedano et al 2014;Napoli et al 2014), even if the use of classical 2-DE for the analysis of proteome is problematic since many proteins resolve poorly in the dimension of the pH gradient.…”

Genome Sequence of Oenococcus oeni OM27, the First Fully Assembled Genome of a Strain Isolated from an Italian Wine

“…The only O. oeni complete genome sequence available is for O. oeni PSU-1 (5), while the assembled genome sequences of 13 O. oeni strains were recently deposited in the GenBank database (6). For O. oeni strain ATCC BAA-1163, a proteome reference map is also available (7). Recently, the role of cultivar, vintage, and climate in the microbial biogeography of wine grapes has been clarified, corroborating the existence of a “microbial terroir” for wine grapes (8, 9), an important concept for fermented food and beverages with a geographical indication status (10–13).…”

Draft Genome Sequence of Oenococcus oeni Strain X 2 L (CRL1947), Isolated from Red Wine of Northwest Argentina

“…However, the harsh wine conditions represent a challenge to the survival and MLF of O. oeni . Therefore, a better understanding of the molecular mechanisms related to the stress tolerance of O. oeni is important for the selection of strains as culture starters ( 4 , 5 ). Genome sequences of several O. oeni strains are available in the GenBank database, with the O. oeni PSU-1 genome being the only complete sequence reported ( 6 – 8 ).…”