A Parallel Micro Turbulent Flow Chromatography-Tandem Mass Spectrometry Method for the Analysis of a Pharmaceutical Compound in Plasma

Principles and Applications of LC-MS/MS for the Quantitative Bioanalysis of Analytes in Various Biological Samples

“…Turbulent flow chromatography (TFC) columns marketed by Cohesive Technologies are widely used for this purpose. Minimum or no sample pre-treatment is required and significant sample preparation time is saved (Chassaing et al, 2005). Another commonly used on-line SPE sorbent material is restricted access material (RAM).…”

Development of a μ-turbulent flow chromatography focus mode method for drug quantitation in discovery bioanalysis

“…The preparation steps prior to sample injection were continued in the format previously described by our group [14] where dilution 1:1 (v/v) in 1 M monochloroacetic acid buffer containing 10% organic helps to release protein bound drug material and keep it in solution. This type of acidification weakly denatures the protein but results in no visible protein precipitation within the sample, enabling direct injection.…”

“…Further useful applications of TFC have been the setup of high throughput GLP validated assays to analyse pharmaceutical compounds entering the development stage [14][15][16] and clinical monitoring of drug exposure levels in hospital patients [17,18]. However, in each of these cases method development time is significantly extended to optimise the quantitation to the analyte/s of interest.…”

Development of a quantitative LC–MS/MS analytical method coupled with turbulent flow chromatography for digoxin for the in vitro P-gp inhibition assay

“…TFC provides an on-line extraction sample clean up that is automated and takes place in real time during the course of an injection thus eliminating manual sample preparation techniques such as liquid-liquid extraction, off-line solid phase extraction, and protein precipitation [12][13][14][15][16][17][18][19][20][21]. Many recent publications utilizing TFC and the combined specificity of selected reaction monitoring through use of triple quadrupole mass spectrometry offers proof that this analytical approach is well accepted [22][23][24][25][26][27][28]. The development of a LC-MS/MS analytical method for digoxin for the P-gp inhibition assay has several other advantages: potential ease-of-transfer of the assay for higher throughput applications, only digoxin is being monitored thus the LC-MS/MS assay can be run continuously without optimizing for new compounds, there are no analytical issues associated with metabolism (i.e.…”