A parallel mechanism underlying frizzle in domestic chickens

Guo, Xing; Li, Yanqing; Wang, Ming-Shan; Wang, Zhibin; Zhang, Quan; Shao, Yong; Jiang, Runshen; Wang, Sheng; Ma, Chendong; Murphy, Robert W.; Wang, Guang-Qin; Dong, Jing; Zhang, Li; Wu, Dong‐Dong; Du, Bin; Peng, Min‐Sheng; Zhang, Yaping

doi:10.1093/jmcb/mjy037

Cited by 10 publications

(13 citation statements)

References 8 publications

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“…A genome-wide association study (GWAS) on the F2 generation produced by crossing Qilin Chickens and Huaixiang Chickens showed that a 15-bp deletion in exon 3 of the KRT75L4 gene caused the frizzle phenotype (Dong et al 2018). A similar result was obtained in a comparative population genomic analysis by comparing multiple indigenous Chinese chicken breeds, and the 15-bp indel was shown to be the causative mutation responsible for the frizzle feather phenotype by injecting the lentivirus into the feather follicles (Guo et al 2018).…”

Section: Introductionmentioning

confidence: 66%

“…Mutations in keratin-related genes can lead to the abnormal animal hair development (Wang et al 2019). Both the deletion of 69-bp in KRT75 and the 15-bp deletion in KRT75L4 can lead to the frizzle feather phenotype, and the ectopic expression of these two mutations in vivo leads to the abnormal development of regenerated feathers (Ng et al 2012;Dong et al 2018;Guo et al 2018). Interestingly, the frizzle trait caused by the 69-bp deletion in KRT75 can be observed in 1-month-old chickens but not in newly hatched chicks (Ng et al 2012), whereas the frizzle feather induced by the 15-bp deletion in KRT75L4 is present in 1-day-old chicks.…”

Section: Discussionmentioning

confidence: 99%

“…All heterozygous individuals (Ff) and homozygous individuals (FF) display the frizzle phenotypes, and the feathers from homozygous individuals are curlier than those from heterozygous individuals (Adomako et al 2014). A chicken breed found in China called Qilin appears to display curled feathers, whereas the breeds considered in previous reports present a normal feather appearance (Dong et al 2018;Guo et al 2018). A previous study that examined a crossed line showed that a member of the a-keratin gene family on chicken chromosome 33 called KRT6A (referred to KRT75 in the original publication), was responsible for frizzle feather phenotype (Ng et al 2012), and a 69-bp deletion within the splicing site at exon 5/intron 5 of KRT6A resulted in the curled appearance of the frizzle feather.…”

Section: Introductionmentioning

confidence: 97%

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Deletion in KRT75L4 linked to frizzle feather in Xiushui Yellow Chickens

Chen

El‐Senousey

et al. 2021

Animal Genetics

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Bird feathers are the product of interactions between natural and artificial selection. Feather-related traits are important for chicken selection and breeding. Frizzle feather is characterized by the abnormally development of feathers in chickens. In the current study, frizzle feather characteristics were observed in a local breed called Xiushui Yellow Chicken in Jiangxi, China. To determine the molecular mechanisms that underlie frizzle feather in Xiushui Yellow Chicken, four populations of three breeds (Xiushui Yellow Chicken with frizzle feathers, Xiushui Yellow Chicken with normal feathers, Guangfeng White-Ear Yellow Chicken, and Ningdu Yellow Chicken) were selected for whole-genome resequencing. Using a comparative genome strategy and genome-wide association study, a missense mutation (g.5281494A>G) and a 15-bp deletion (g.5285437-5285451delGATGCCGGCAGGACG) in KRT75L4 were identified as candidate mutations associated with frizzle feather in Xiushui Yellow Chicken. Based on genotyping performed in a large Xiushui Yellow Chicken population, the g.5285437-5285451delGATGCCGGCAGGACG mutation in KRT75L4 was confirmed as the putative causative mutation of frizzle feather. These results deepen the understanding of the molecular mechanisms responsible for frizzle feather, as well as facilitating the molecular detection and selection of the feather phenotype in Xiushui Yellow Chickens.

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Section: Introductionmentioning

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Deletion in KRT75L4 linked to frizzle feather in Xiushui Yellow Chickens

Chen

El‐Senousey

et al. 2021

Animal Genetics

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“…We herein detected common molecular network and functional pathways involved in abdominal fat deposition after network construction and comparison between different chicken lines. It's a common phenomenon that similar traits of animals in different populations could be under convergent selection, i.e., different genes but pathways of similar molecular functions are selected by evolutionary forces or artificial selective pressure, such as convergent selection signatures in sheep and goat (Alberto et al, 2018), and the frizzle phenotype in chickens (Dong et al, 2018;Guo et al, 2018). Here, our network analysis also showed that, though different chicken lines were divergently selected for abdominal fat content, network modules of similar molecular functionality were detected.…”

Section: Discussionmentioning

confidence: 99%

Common Gene Modules Identified for Chicken Adiposity by Network Construction and Comparison

et al. 2020

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Excessive fat deposition can cause chicken health problem, and affect production efficiency by causing great economic losses to the industry. However, the molecular underpinnings of the complex adiposity trait remain elusive. In the current study, we constructed and compared the gene co-expression networks on four transcriptome profiling datasets, from two chicken lines under divergent selection for abdominal fat contents, in an attempt to dissect network compositions underlying adipose tissue growth and development. After functional enrichment analysis, nine network modules important to adipogenesis were discovered to be involved in lipid metabolism, PPAR and insulin signaling pathways, and contained hub genes related to adipogenesis, cell cycle, inflammation, and protein synthesis. Moreover, after additional functional annotation and network module comparisons, common sub-modules of similar functionality for chicken fat deposition were identified for different chicken lines, apart from modules specific to each chicken line. We further validated the lysosome pathway, and found TFEB and its downstream target genes showed similar expression patterns along with chicken preadipocyte differentiation. Our findings could provide novel insights into the genetic basis of complex adiposity traits, as well as human obesity and related metabolic diseases.

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“…The different loci contributing to dark comb probably present a case of parallel selection at the molecular level under adaptive selection. As a matter of fact, the common phenotypes in different breeds are probably caused by various loci, such as frizzle feather (Guo et al, 2018), blue eggshell (Wragg et al, 2013), polydactyly (Zhang et al, 2016), and dwarfism (Wang et al, 2017) in different chicken breeds. Thus, considering the genetic architectures of different chicken populations is indispensable even though they have the same phenotypic trait.…”

Section: Discussionmentioning

confidence: 99%

Genomic Analysis Reveals Pleiotropic Alleles at EDN3 and BMP7 Involved in Chicken Comb Color and Egg Production

Dong

Zhang

et al. 2019

Front. Genet.

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Artificial selection is often associated with numerous changes in seemingly unrelated phenotypic traits. The genetic mechanisms of correlated phenotypes probably involve pleiotropy or linkage of genes related to such phenotypes. Dongxiang blue-shelled chicken, an indigenous chicken breed of China, has segregated significantly for the dermal hyperpigmentation phenotype. Two lines of the chicken have been divergently selected with respect to comb color for over 20 generations. The red comb line chicken produces significantly higher number of eggs than the dark comb line chicken. The objective of this study was to explore potential mechanisms involved in the relationship between comb color and egg production among chickens. Based on the genome-wide association study results, we identified a genomic region on chromosome 20 involving EDN3 and BMP7 , which is associated with hyperpigmentation of chicken comb. Further analyses by selection signatures in the two divergent lines revealed that several candidate genes, including EDN3 , BMP7 , BPIFB3 , and PCK1 , closely located on chromosome 20 are involved in the development of neural crest cell and reproductive system. The two genes EDN3 and BMP7 have known roles in regulating both ovarian function and melanogenesis, indicating the pleiotropic effect on hyperpigmentation and egg production in blue-shelled chickens. Association analysis for egg production confirmed the pleiotropic effect of selected loci identified by selection signatures. The study provides insights into phenotypic evolution due to genetic variation across the genome. The information might be useful in the current breeding efforts to develop improved breeds for egg production.

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A parallel mechanism underlying frizzle in domestic chickens

Cited by 10 publications

References 8 publications

Deletion in KRT75L4 linked to frizzle feather in Xiushui Yellow Chickens

Deletion in KRT75L4 linked to frizzle feather in Xiushui Yellow Chickens

Common Gene Modules Identified for Chicken Adiposity by Network Construction and Comparison

Genomic Analysis Reveals Pleiotropic Alleles at EDN3 and BMP7 Involved in Chicken Comb Color and Egg Production

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