A parallel comparison of T-cell clonality assessment between an in-house PCR assay and the BIOMED-2 assay leading to an efficient and cost-effective strategy

Kuo, Szu-Yin; Liu, Hongxiang; Liao, Yung-Liang; Chang, Sheng‐Tsung; Hsieh, Yu‐Chia; Bandoh, Betty Angela Nuako; Du, Ming‐Qing; Chuang, Shih‐Sung

doi:10.1136/jcp.2010.086637

Cited by 17 publications

(15 citation statements)

References 37 publications

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“…We performed in situ hybridization for EBV-encoded mRNA (EBER) in an autostainer using a polymer-based detection system with an EBV-specific probe (Bond MAX, Vision BioSystems Ltd, Mount Waverley, Australia). Polymerase chain reaction analysis for TCR-GR using paraffin sections was performed as previously described [16].…”

Section: Immunohistochemical Studymentioning

confidence: 99%

Extranodal natural killer/T-cell lymphoma, nasal type in Taiwan: a relatively higher frequency of T-cell lineage and poor survival for extranasal tumors

et al. 2015

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Section: Immunohistochemical Studymentioning

confidence: 99%

Extranodal natural killer/T-cell lymphoma, nasal type in Taiwan: a relatively higher frequency of T-cell lineage and poor survival for extranasal tumors

et al. 2015

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“…, 2009). All seven neoplasms with T‐LGL leukaemia were immunophenotyped by flow cytometry, and the tumour cells were all clonal for T‐cell receptor gene rearrangement using polymerase chain reaction‐based assay (Kuo et al. , 2011).…”

Section: Resultsmentioning

confidence: 99%

The spectrum of T‐cell and natural killer/T‐cell neoplasms with leukaemic presentation in a single institution in Taiwan

Chang

Hsieh

Kuo

et al. 2012

Int J Lab Hematology

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T cell and natural killer (NK)/T-cell neoplasms are rare and may occasionally present as leukaemia. We retrospectively searched T cell and NK/T-cell tumours in a single institution in Taiwan from January 2000 to December 2009 and identified 137 (19.1%) patients with T cell and NK/T-cell tumours among 718 patients with lymphoid neoplasms. Among these 137 patients, 18 (13.1%) presented with leukaemia including T-lymphoblastic lymphoma/leukaemia (T-LBL), T-cell prolymphocytic leukaemia, aggressive NK-cell leukaemia, adult T-cell lymphoma/leukaemia (ATLL), T-cell large granular lymphocytic (T-LGL) leukaemia and unspecified peripheral T-cell lymphoma. Cases with concurrent lymphoma, higher absolute leukaemic cell counts and elevated lactate dehydrogenase level carried a poorer prognosis. The survival was dichotomous, with a very poor prognosis for patients with T-LBL, T-cell prolymphocytic leukaemia, aggressive NK-cell leukaemia, ATLL in acute phase and unspecified peripheral T-cell lymphoma, while those with T-LGL leukaemia and ATLL in chronic phase had a favourable outcome.

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“…We adapted the cost-effective and efficient strategy to combine primers published by McCarthy and colleagues and commercial TCRG BIOMED-2 primers (Kuo et al, 2011). The detection rate of TCR gene rearrangement by this method reaches 91%, whereas 85% can be achieved by all six BIOMED-2 reactions and 94% by combining the primers used by McCarthy et al and all BIOMED-2 reactions (Kuo et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…T cell receptor rearrangement assay PCR reactions to examine T cell receptor (TCR) gene rearrangement were performed by a combination of TCR gamma-chain gene (TCRG) primers designed by McCarthy et al and a commercial TCRG gene clonality assay (Invivoscribe Technologies) (Kuo et al, 2011;McCarthy et al, 1992). …”

Section: Determination Of Karyotypesmentioning

confidence: 99%

Induced Pluripotent Stem Cell Reprogramming by Integration-Free Sendai Virus Vectors from Peripheral Blood of Patients with Craniometaphyseal Dysplasia

Chen

Fukuda

Fusaki

et al. 2013

Cellular Reprogramming

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Studies of rare genetic bone disorders are often limited due to unavailability of tissue specimens and the lack of animal models fully replicating phenotypic features. Craniometaphyseal dysplasia (CMD) is a rare monogenic disorder characterized by hyperostosis of craniofacial bones concurrent with abnormal shape of long bones. Mutations for autosomal dominant CMD have been identified in the ANK gene (ANKH). Here we describe a simple and efficient method to reprogram adherent cells cultured from peripheral blood to human induced pluripotent stem cells (hiPSCs) from eight CMD patients and five healthy controls. Peripheral blood mononuclear cells (PBMCs) were separated from 5-7 mL of whole blood by Ficoll gradient, expanded in the presence of cytokines and transduced with Sendai virus (SeV) vectors encoding OCT3/4, SOX2, KLF4, and c-MYC. SeV vector, a cytoplasmic RNA vector, is lost from host cells after propagation for 10-13 passages. These hiPSCs express stem cell markers, have normal karyotypes, and are capable of forming embryoid bodies in vitro as well as teratomas in vivo. Further differentiation of these patient-specific iPSCs into osteoblasts and osteoclasts can provide a useful tool to study the effects CMD mutations on bone, and this approach can be applied for disease modeling of other rare genetic musculoskeletal disorders.

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A parallel comparison of T-cell clonality assessment between an in-house PCR assay and the BIOMED-2 assay leading to an efficient and cost-effective strategy

Abstract: As the in-house primers were custom made and are much cheaper than the commercial kits, the authors concluded that this four-tube strategy was cost-effective and efficient for TCR-GR clonality assessment.

Cited by 17 publications

References 37 publications

Extranodal natural killer/T-cell lymphoma, nasal type in Taiwan: a relatively higher frequency of T-cell lineage and poor survival for extranasal tumors

Extranodal natural killer/T-cell lymphoma, nasal type in Taiwan: a relatively higher frequency of T-cell lineage and poor survival for extranasal tumors

The spectrum of T‐cell and natural killer/T‐cell neoplasms with leukaemic presentation in a single institution in Taiwan

Induced Pluripotent Stem Cell Reprogramming by Integration-Free Sendai Virus Vectors from Peripheral Blood of Patients with Craniometaphyseal Dysplasia

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