A paradigm shift in biomass technology from complete to partial cellulose hydrolysis: lessons learned from nature

Chen, Rachel

doi:10.1080/21655979.2014.1004019

Cited by 21 publications

(16 citation statements)

References 23 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…with several sugar transporters (CDT-1 from N. crassa and Lac12 from Kluyveromyces lactis, etc. )have been introduced into S. cerevisiae for successful ethanol production from cellobiose [1,2,[8][9][10][11][12][13][14]. Whereas the hydrolytic pathway is known to spend 2 ATP molecules to initiate glycolysis with cellobiose (cellobiose + H 2 O + 2 ATP → 2 glucose-6-phosphate + 2 ADP), the phosphorolytic pathway is known to have energetic advantages with the expense of only 1 ATP molecule (cellobiose + phosphate + ATP → 2 glucose-6-phosphate + ADP), suggesting that the phosphorolytic pathway would provide several benefits (e.g., higher cell growth yield and higher ethanol yield by saving energy) compared with the hydrolytic pathway under anaerobic and stressful conditions [9,13,15,16].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Ethanol Production Activity by Engineered Saccharomyces cerevisiae Fermenting Cellobiose through the Phosphorolytic Pathway in Simultaneous Saccharification and Fermentation of Cellulose

Lee¹,

Liu²

2017

Journal of Microbiology and Biotechnology

View full text Add to dashboard Cite

In simultaneous saccharification and fermentation (SSF) for production of cellulosic biofuels, engineered Saccharomyces cerevisiae capable of fermenting cellobiose has provided several benefits, such as lower enzyme costs and faster fermentation rate compared with wild-type S. cerevisiae fermenting glucose. In this study, the effects of an alternative intracellular cellobiose utilization pathway-a phosphorolytic pathway based on a mutant cellodextrin transporter (CDT-1 (F213L)) and cellobiose phosphorylase (SdCBP)-was investigated by comparing with a hydrolytic pathway based on the same transporter and an intracellular β-glucosidase (GH1-1) for their SSF performances under various conditions. Whereas the phosphorolytic and hydrolytic cellobiose-fermenting S. cerevisiae strains performed similarly under the anoxic SSF conditions, the hydrolytic S. cerevisiae performed slightly better than the phosphorolytic S. cerevisiae under the microaerobic SSF conditions. Nonetheless, the phosphorolytic S. cerevisiae expressing the mutant CDT-1 showed better ethanol production than the glucose-fermenting S. cerevisiae with an extracellular β-glucosidase, regardless of SSF conditions. These results clearly prove that introduction of the intracellular cellobiose metabolic pathway into yeast can be effective on cellulosic ethanol production in SSF. They also demonstrate that enhancement of cellobiose transport activity in engineered yeast is the most important factor affecting the efficiency of SSF of cellulose. S S 1650 Lee and Jin J. Microbiol. Biotechnol.

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation of Ethanol Production Activity by Engineered Saccharomyces cerevisiae Fermenting Cellobiose through the Phosphorolytic Pathway in Simultaneous Saccharification and Fermentation of Cellulose

Lee¹,

Liu²

2017

Journal of Microbiology and Biotechnology

View full text Add to dashboard Cite

show abstract

“…d ‐cellobiose is a disaccharide composed of two β‐glucose monomers linked by a β(1 → 4) bond. It is a by‐product of cellulose saccharification with standard commercial mixtures of cellulases but becomes a predominant product when β‐glucosidase is omitted from the cocktail (Chen, 2015). Well‐defined microbial hosts capable of efficient cellobiose utilization are therefore desirable because they can be applied in simultaneous saccharification and fermentation of cellulose for production of VAC while the process cost is reduced as addition of expensive β‐glucosidase is not needed (Ha et al , 2011; Chen, 2015; Parisutham et al , 2017).…”

Section: Resultsmentioning

confidence: 99%

Biotransformation of d‐xylose to d‐xylonate coupled to medium‐chain‐length polyhydroxyalkanoate production in cellobiose‐grown Pseudomonas putida EM42

Dvořák

Kováč

Lorenzo

2020

Microbial Biotechnology

View full text Add to dashboard Cite

Co-production of two or more desirable compounds from low-cost substrates by a single microbial catalyst could greatly improve the economic competitiveness of many biotechnological processes. However, reports demonstrating the adoption of such co-production strategy are still scarce. In this study, the ability of genome-edited strain Pseudomonas putida EM42 to simultaneously valorize D-xylose and D-cellobiosetwo important lignocellulosic carbohydratesby converting them into the platform chemical D-xylonate and medium-chain-length polyhydroxyalkanoates, respectively, was investigated. Biotransformation experiments performed with P. putida resting cells showed that promiscuous periplasmic glucose oxidation route can efficiently generate extracellular xylonate with a high yield.Xylose oxidation was subsequently coupled to the growth of P. putida with cytoplasmic b-glucosidase BglC from Thermobifida fusca on D-cellobiose. This disaccharide turned out to be a better co-substrate for xylose-to-xylonate biotransformation than monomeric glucose. This was because unlike glucose, cellobiose did not block oxidation of the pentose by periplasmic glucose dehydrogenase Gcd, but, similarly to glucose, it was a suitable substrate for polyhydroxyalkanoate formation in P. putida. Coproduction of extracellular xylose-born xylonate and intracellular cellobiose-born medium-chain-length polyhydroxyalkanoates was established in proof-ofconcept experiments with P. putida grown on the disaccharide. This study highlights the potential of P. putida EM42 as a microbial platform for the production of xylonate, identifies cellobiose as a new substrate for mcl-PHA production, and proposes a fresh strategy for the simultaneous valorization of xylose and cellobiose.

show abstract

“…D-cellobiose is a disaccharide composed of two β-glucose monomers linked by a β(1→4) bond. It is a by-product of cellulose saccharification with standard commercial mixtures of cellulases but becomes a predominant product when β-glucosidase is omitted from the cocktail (Chen, 2015). Well-defined microbial hosts capable of efficient cellobiose utilisation are therefore desirable because they can be applied in simultaneous saccharification and fermentation of cellulose for production of VAC while the process cost is reduced as addition of expensive β-glucosidase is not needed (Ha et al , 2011; Chen, 2015; Parisutham et al , 2017).…”

Section: Resultsmentioning

confidence: 99%

Biotransformation of D-xylose to D-xylonic acid coupled to medium chain length polyhydroxyalkanoate production in cellobiose-grownPseudomonas putidaEM42

Dvořák

Kováč

Lorenzo

2019

Preprint

View full text Add to dashboard Cite

1Co-production of two or more desirable compounds from low-cost substrates by a single 2 microbial catalyst could greatly improve the economic competitiveness of many 3 biotechnological processes. However, reports demonstrating the adoption of such co-4 production strategy are still scarce. In this study, the ability of genome-edited strain 5 Psudomonas putida EM42 to simultaneously valorise D-xylose and D-cellobiose -two 6 important lignocellulosic carbohydrates -by converting them into the platform chemical D-7 xylonic acid and medium chain length polyhydroxyalkanoates, respectively, was investigated. 8 Biotransformation experiments performed with P. putida resting cells showed that 9 promiscuous periplasmic glucose oxidation route can efficiently generate extracellular 10 xylonate with high yield reaching 0.97 g per g of supplied xylose. Xylose oxidation was 11 subsequently coupled to the growth of P. putida with cytoplasmic -glucosidase BglC from 12 Thermobifida fusca on D-cellobiose. This disaccharide turned out to be a better co-substrate 13 for xylose-to-xylonate biotransformation than monomeric glucose. This was because unlike 14 glucose, cellobiose did not block oxidation of the pentose by periplasmic glucose 15 dehydrogenase Gcd, but, similarly to glucose, it was a suitable substrate for 16 polyhydroxyalkanoate formation in P. putida. Co-production of extracellular xylose-born 17 xylonate and intracellular cellobiose-born medium chain length polyhydroxyalkanoates was 18 established in proof-of-concept experiments with P. putida grown on the disaccharide. This 19 study highlights the potential of P. putida EM42 as a microbial platform for the production of 20 xylonic acid, identifies cellobiose as a new substrate for mcl-PHA production, and proposes a 21 fresh strategy for the simultaneous valorisation of xylose and cellobiose.22 23 24 3

show abstract

A paradigm shift in biomass technology from complete to partial cellulose hydrolysis: lessons learned from nature

Cited by 21 publications

References 23 publications

Evaluation of Ethanol Production Activity by Engineered Saccharomyces cerevisiae Fermenting Cellobiose through the Phosphorolytic Pathway in Simultaneous Saccharification and Fermentation of Cellulose

Evaluation of Ethanol Production Activity by Engineered Saccharomyces cerevisiae Fermenting Cellobiose through the Phosphorolytic Pathway in Simultaneous Saccharification and Fermentation of Cellulose

Biotransformation of d‐xylose to d‐xylonate coupled to medium‐chain‐length polyhydroxyalkanoate production in cellobiose‐grown Pseudomonas putida EM42

Biotransformation of D-xylose to D-xylonic acid coupled to medium chain length polyhydroxyalkanoate production in cellobiose-grownPseudomonas putidaEM42

Contact Info

Product

Resources

About