A Paracorporeal Rat Heart Model for Ischemic and Reperfusion Studies

Hultman, Jan; Forsberg, J. O.; Hansson, H; Ronquist, Gunnar

doi:10.3109/03009738209178428

Cited by 9 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A paracorporeal heart model modified for the rat was employed [14]. The model consisted o f four main parts ( fig.…”

Section: Experimental Modelmentioning

confidence: 99%

Myocardial High-Energy Phosphates and Function under Different Postischemic Conditions

Hultman¹,

Ronquist²

1984

Eur Surg Res

View full text Add to dashboard Cite

Infusion of phosphoenolpyruvate together with adenosine triphosphate early during reperfusion after ischemia increased the energy content and the left ventricular isovolumic performance in a paracorporeal rat heart model. Excised hearts were subjected to 15 min of complete global ischemia at 37 °C before reperfusion. During reperfusion they were either nonworking (empty-beating) or working (performing left ventricular isovolumic work) between 20 and 40 min of reperfusion before freeze-clamping. Early during reperfusion the excised hearts were either supplemented with 144 µmol phosphoenolpyruvate and 0.67 µmol adenosine triphosphate or nonsupplemented (plain saline) administered in a pulsatile arterial blood flow. The supplemented nonworking group showed significantly higher creatine phosphate and adenosine triphosphate contents compared to the nonsupplemented group. However, under postischemic working conditions there were no significant differences in high-energy phosphates between the supplemented and the nonsupplemented groups in spite of significantly better left ventricular isovolumic performance in the supplemented group. Concomitantly the efflux of creatine kinase isoenzyme MB indicating cellular damage was significantly less for this group. The data presented here favor the view of a possible compartmentalized adenosine triphosphate pool with a high metabolic turnover close to the plasma membrane.

show abstract

“…A paracorporeal heart model modified for the rat was employed [14]. The model consisted o f four main parts ( fig.…”

Section: Experimental Modelmentioning

confidence: 99%

Myocardial High-Energy Phosphates and Function under Different Postischemic Conditions

Hultman¹,

Ronquist²

1984

Eur Surg Res

View full text Add to dashboard Cite

show abstract

“…A paracorporeal heart model modified for the rat was used [8], The model consists of three main parts ( fig. I): (l)th e supporting rat; (2) the excised heart, and (3) the retransfusion system.…”

Section: Experimental Modelmentioning

confidence: 99%

Myocardial Energy Restoration of Ischemic Damage by Administration of Phosphoenolpyruvate during Reperfusion

et al. 1983

View full text Add to dashboard Cite

The myocardial energy restoration of ischemic damage by administration of phosphoenolpyruvate during reperfusion was investigated in a paracorporeal heart model modified in the rat. Excised hearts were subjected to 15 min of complete global ischemia at 37 °C before pulsatile blood reperfusion for 30 min. One group (n = 7) was supplemented with 67 µmol of phosphoenolpyruvate and 0.67 µmol of adenosine triphosphate dissolved in 10 ml of saline and another with plain saline (nonsupplemented) during reperfusion. All hearts were freeze-clamped after 30 min of reperfusion and subjected to energy content analysis. The adenylate charge potential was 0.91 ± 0.01 (mean ± SD) for the supplemented and 0.85 ± 0.06 (mean ± SD) for the nonsupplemented group. This difference was significant (p < 0.02). Concomitantly the outflow of creatine kinase was less for the supplemented group. The translocation of phosphoenolpyruvate into the myocardial cells seems possible by blood supplementation of a lower dose of adenosine triphosphate.

show abstract

“…For the most accurate reproduction of the in vivo situation, donor animals can be used to provide blood for the isolated heart preparation (23,40).…”

Section: Perfusatesmentioning

confidence: 99%

Use of the Isolated Perfused Heart for Evaluation of Cardiac Toxicity

et al. 1990

View full text Add to dashboard Cite

The isolated perfused rat heart model can be used to evaluate cardiotoxicity, and is especially useful in distinguishing direct vs indirect cardiac injury. Various perfusion systems can be used to characterize the pathophysiologic as well as morphologic changes induced by drugs or chemicals of interest. The isolated perfused heart was used in the studies described herein to characterize the mechanism of allylamine cardiotoxicity. Rat hearts were perfused with Krebs-Henseleit buffer containing 10 mm allylamine and a latex balloon was inserted into the left ventricle to monitor pressure. Coronary flow in hearts perfused with 10 mm allylamine was similar to control hearts at 5, 10, and 30 min, but was reduced by 1 hr (11.5 ± 0.6 ml/min/g wet heart weight vs 16.0 ± 0.7, p < 0.01). Peak left ventricular systolic pressure increased in hearts perfused with allylamine for 5 min (156 ± 8 mm Hg vs 103 ± 9, p < 0.01), but by 2 hr was decreased compared to controls (89 ± 6 vs 105 ± 5, p < 0.05). End diastolic pressure was markedly increased at 2 hr (58 ± 3 vs 4 ± 0.8, p < 0.01). Morphologically, allylamine perfused hearts exhibited significant contraction band changes as well as numerous cells with marked swelling of the sarcoplasmic reticulum. The findings in this study suggest that allylamine produces direct myocardial damage that appears to be independent of coronary flow. These studies demonstrate that the isolated perfused rat heart model can be used to evaluate mechanisms of acute cardiotoxicity.

show abstract

A Paracorporeal Rat Heart Model for Ischemic and Reperfusion Studies

Cited by 9 publications

References 16 publications

Myocardial High-Energy Phosphates and Function under Different Postischemic Conditions

Myocardial High-Energy Phosphates and Function under Different Postischemic Conditions

Myocardial Energy Restoration of Ischemic Damage by Administration of Phosphoenolpyruvate during Reperfusion

Use of the Isolated Perfused Heart for Evaluation of Cardiac Toxicity

Contact Info

Product

Resources

About