A panel of oxidative stress assays does not provide supplementary diagnostic information in Behcet's disease patients

Abstract

BackgroundRecent findings suggest a role of oxidative stress in the pathogenesis of Behcet's disease (BD), but the utility of oxidative stress-associated assays in offering diagnostic information or in the monitoring of disease activity is largely unassessed.Objective and methodsWe aimed to measure oxidative and inflammatory markers, along with the markers of reactive nitrogen species, S-nitrosothiols and 3-nitrotyrosine, in BD patients (n = 100) and healthy volunteers (n = 50). These markers were evaluated in regard to their role in the pathogenesis of BD as well as their relation to clinical presentation, disease activity and duration.ResultsMedian values for erythrocyte sedimentation rate (ESR), C-reactive protein, leukocyte count, and IL-18 levels, as well as myeloperoxidase (MPO) activity, were statistically higher in the patient group compared to controls. Some inflammation markers (ESR, neutrophil and leukocyte counts) were statistically higher (p < 0.05) in the active period. In contrast, oxidative stress-associated measures (erythrocyte lipid peroxidation, antioxidant enzymes and measures of serum antioxidant capacity), revealed no statistically significant differences between the median values in BD patients versus healthy control subjects (p > 0.05 in all statistical comparisons), nor was there any difference in median levels of these oxidative stress markers in active disease versus disease remission. S-nitrosothiols and 3-nitrotyrosine were undetectable in BD plasma.ConclusionsThe application of oxidative stress-associated measures to BD blood samples offered no supplemental diagnostic or disease activity information to that provided by standard laboratory measures of inflammation. S-nitrosothiols and 3-nitrotyrosine appeared not to be markers for active BD; thus the search for biochemical markers that will indicate the active period should be continued with larger studies.

Assessment of total (anti)oxidant status in goat kids

“…In humans, a correlation between TOS e NO x levels was also shown (Padhy et al, 2015), whereas in our study the correlation was not observed (r = 0.370; p = 0.10). NO x levels were also significantly correlated with TAA, as well as MPO activity with TAC, as previously shown in human disorders, in which the increased NO x levels and MPO activity are suspected to be involved in OS (Akcay et al, 2012;Schuh et al, 2018).…”

“…Although both ROMs and TOS assays are based on iron-mediated mechanisms, the principles of detection are quite different, and this could be the reason for the lack of correlation (Erel, 2005;Jansen and Ruskovska, 2013). In reality, the ROM test, given by Alberti et al (2000) as a specific measurement of hydroperoxide levels, was correlated neither with TOS nor MPO in the present results. In contrast, a highly significant correlation with CP oxidase activity, a glycoprotein known as a potent physiologic inhibitor of myeloperoxidase (Chapman et al, 2013), was evidenced in goat sera.…”

“…Reactive oxygen metabolites (ROMs) were analysed as described by Alberti et al (2000). The method measures the hydroperoxide levels in a sample, and it is based on the principle that hydroperoxides, in the presence of iron ions released from proteins by an acidic buffered solution, are able to generate alkoxyl (R-O*) and peroxyl (R-OO*) radicals.…”

“…Generally speaking, these assays are based on the principle that the antioxidant potential of a biological sample is measured as the content of free radicals scavenged by a test solution or the capacity to reduce an oxidized chemical substance, whereas the oxidant potential is measured as the content of substances able to oxidize a chemical compound in a test solution (Alberti et al, 2000;Erel, 2005;Ghiselli et al, 2000). Some of these assays have recently been tested in biological samples of livestock other than serum or plasma, such as saliva in sheep (Rubio et al, 2019) and the exhaled breath condensate in horses (Po et al, 2013).…”

Levels of F2isoprostane in Behcet's disease: Correlation with cardiometabolic risk factors

“…[8][9][10] Various oxidative stress markers and oxidative states have also been studied in patients with BD with inconsistent results. [11][12][13] Oxidative stress and inflammatory processes are the main features of many acute and chronic diseases, such as cancer, lung disease, cardiovascular disease, and rheumatic disease, as well as normal aging processes.…”