A Pancreatic Lipase Inhibitory Activity by Mango (Mangifera indica) Leaf Methanolic Extract

Abstract

<p>The objective of this study was to identify pancreatic lipase inhibitory active ingredients of mango leaves, and to examine a relationship between leaves maturation and pancreatic lipase inhibitory activity. A methanolic extract of old dark green mango leaves (OML-ext) showed a porcine pancreatic lipase inhibitory activity. The pancreatic lipase inhibitory activity of OML-ext was attributable to 3-C-<em>β</em>-D-glucosyl-2,4,4’,6-tetrahydroxybenzophenone (<strong>2</strong>) and mangiferin (<strong>1</strong>). The pancreatic lipase inhibitory activity of young mango leaf extract was superior to that of old leaf extract. It was suggested that the activity is correlated with the content of <strong>2</strong> in these extract. Considering the amounts of leaves obtained from pruning, old dark green leaves may be a reasonable natural resource for the preparation of ingredients with lipase inhibitory activity.</p>

Inhibition of Advanced Glycation End Products Formation by Mangifera indica Leaf Extract

“…7-25) eluted with CHCl 3 /MeOH 30:1 v/v, 10:1 v/v, 5:1 v/v and 0:10 v/v also showed potent activity. Further purification of fraction II by a preparative HPLC led to isolation of 1 and 2 as described in the preceding paper (Itoh et al, 2016).…”

“…Irwin) were collected according to the preceding paper (Itoh et al, 2016). In order to describe accurately the color of young mango leaves, we describe the color of the leaves as dark reddish brown in this paper instead of dark brown in the preceding paper (Itoh et al, 2016). …”

“…Linear calculation curves in the range of 0.02 to 0.1 nmol were made from the peak areas analyzed at 520 nm, and the correlation coefficient was 0.992. The HPLC determination of 1 and 2 in each leaf extract was described in the preceding paper (Itoh et al, 2016).…”

“…Methanolic extracts of young dark reddish brown leaves (this extract is abbreviated as YDL-ext throughout this paper), young yellow leaves (YYL-ext), and old dark green leaves (OML-ext) were obtained according to the preceding paper (Itoh et al, 2016).…”

“…In our preceding paper (Itoh et al, 2016), we reported that mango (Mangifera indica Linne) leaf extracts exhibited pancreatic lipase inhibitory activities, and a part of the activity of leaf extract was attributable to C-glucosyl-polyphenols, such as 3-C-β-D-glucosyl-2,4,4',6-tetrahydroxybenzophenone (1) and mangiferin (2), and that dark green mango leaf which was obtained by summer pruning may be a reasonable natural resource for the preparation of ingredients with lipase inhibitory activity. For finding another utility value of pruned mango leaves, we examined the inhibitory effects of mango leaf extracts on AGEs formation.…”