A pan-cancer analysis of ring finger protein 135 and its relationship to triple-negative breast cancer proliferation and metastasis

Abstract

Ring finger protein 135 (RNF135) is an E3 ubiquitin ligase with RING finger domains that plays a crucial role in the development of several forms of cancer. Neither the expression profile of RNF135 nor its importance in the diagnosis of pan-cancer have been elucidated as of yet. With the aid of The Cancer Genome Atlas and Gene Expression Omnibus, we have fully mapped the expression profiles, prognostic relevance, genetic modification, immune cell infiltration, and tumor heterogeneity of RNF135 in 33 malignant tumors. RNF135 was expressed inconsistently in various cancers, and variations in RNF135 expression predicted survival outcomes for cancer patients. There was a strong correlation between the levels of the RNF135 genetic mutation and some tumor progression. In addition, a strong correlation was seen between RNF135 expression and immune cell infiltration, tumor mutation burden, microsatellite instability, and immunoregulators. In contrast, the correlation between RNF135 expression and triple-negative breast cancer was investigated in this study. RNF135 may boost the proliferation, migration, and invasion of TNBC cells, according to cell experiments. RNF135 might be utilized as a biomarker to anticipate how a tumor will behave and may have a significant role in how TNBC cells grow and migrate, according to the findings of this study.

DNA Methylation Biomarker Panels for the Differentiation of Hepatocellular Carcinoma and Cholangiocarcinoma from Liver Metastases from Colorectal Carcinoma and Pancreatic Adenocarcinoma

“…RNF135 belongs to the ring nger protein family, which contains an E3 ubiquitin ligase, and plays a crucial role in antiviral innate immunity (52). In cancer, RNF135 promotes proliferation via the ERK signaling pathway, inhibits tumorigenesis by regulating AKT signaling and affects tumor microenvironment (53)(54)(55)(56). In HCC, RNF135 functions as a tumor suppressor, as promoter hypermethylation negatively correlates with downregulation of RNF135 in HCC tissues, which promotes HCC cell migration and affects the tumor immune microenvironment.…”

Plasma methylated GNB4 and Riplet as a novel dual-marker panel for the detection of hepatocellular carcinoma

“…Recently, it has been reported that DNA methylation of G protein subunit beta 4 (GNB4) can be a potential target for anti-oestrogen resistance treatment of breast cancer and a potential marker for diagnosis and prognosis evaluation of gastric cancer induced by Helicobacter pylori [ 38 , 39 ] The Riplet gene (also known as Ring finger protein 135, RNF135) has also been found to be involved in the regulation of proliferation and metastasis of triple-negative breast cancer [ 40 ], and its promoter methylation is related to the immune invasion and prognosis of HCC [ 41 ]. These studies have shown that methylation of these two genes has an important regulatory role in the occurrence and development of cancer.…”