A pair-rule gene circuit defines segments sequentially in the short-germ insect
            <i>Tribolium castaneum</i>

Choe, Chong Pyo; Miller, Sherry; Brown, Susan J.

doi:10.1073/pnas.0510440103

Cited by 196 publications

(350 citation statements)

References 36 publications

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“…dseveRNA injected and buffer-injected controls were reared for 18-19 h (25°C), when control embryos typically have 15 post-antennal EN stripes. The phenotypes mimicked published results using the same construct for pupal RNAi injections 44 .…”

Section: Methodssupporting

confidence: 78%

“…Because the pair-rule gene eve is required for the cell rearrangements that drive germband elongation in Drosophila 19 , we assayed the behaviour of blastoderm clones in embryos injected with dseveRNA in the early blastoderm (4-5 h AEL). About 100% of the injected embryos share the same phenotype with eve RNA interference (RNAi) embryos generated through maternal RNAi 44 . Clones marked between 60 and 80% egg length in the RNAi embryos do not differ significantly from controls in the amount of cell division (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Changing cell behaviours during beetle embryogenesis correlates with slowing of segmentation

Nakamoto

Hester²,

Constantinou

et al. 2015

Nat Commun

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Segmented animals are found in major clades as phylogenetically distant as vertebrates and arthropods. Typically, segments form sequentially in what has been thought to be a regular process, relying on a segmentation clock to pattern budding segments and posterior mitosis to generate axial elongation. Here we show that segmentation in Tribolium has phases of variable periodicity during which segments are added at different rates. Furthermore, elongation during a period of rapid posterior segment addition is driven by high rates of cell rearrangement, demonstrated by differential fates of marked anterior and posterior blastoderm cells. A computational model of this period successfully reproduces elongation through cell rearrangement in the absence of cell division. Unlike current models of steady-state sequential segmentation and elongation from a proliferative growth zone, our results indicate that cell behaviours are dynamic and variable, corresponding to differences in segmentation rate and giving rise to morphologically distinct regions of the embryo.

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Section: Methodssupporting

confidence: 78%

Section: Resultsmentioning

confidence: 99%

Changing cell behaviours during beetle embryogenesis correlates with slowing of segmentation

Nakamoto

Hester²,

Constantinou

et al. 2015

Nat Commun

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“…Pair rule gene orthologs are among the first genes in all arthropods to be activated in a periodic pattern. (Choe et al, 2006). Repetitive patterns of pair rule gene expression are generated via a regulatory circuit of the three pair rule genes eve, run, and odd.…”

Section: Conservation Of the Segment-polarity Network And The Conservmentioning

confidence: 99%

“…Canonical pair rule patterning, therefore, is in such a twosegmental periodicity and this seems to be conserved at least among holometabolous insects (Davis and Patel, 2003). In such short germ holometabolous insects like the beetle Tribolium castaneum, the initial expression patterns of pair rule gene orthologs are also in a double segmental periodicity (Sommer and Tautz, 1993;Patel et al, 1994;Choe et al, 2006). In addition, gene inactivation leads to typical pair rule phenotypes for orthologs of the pair rule genes even skipped (eve), sloppy paired (slp), and paired (prd) in Tribolium (Schröder et al, 1999;Maderspacher et al, 1998;Choe and Brown, 2007).…”

Section: The Transition From a Non-periodic To A Periodic Pattern: Ormentioning

confidence: 99%

Evolutionary conservation and divergence of the segmentation process in arthropods

Damen

2007

Developmental Dynamics

153

122

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A fundamental characteristic of the arthropod body plan is its organization in metameric units along the anterior-posterior axis. The segmental organization is laid down during early embryogenesis. Our view on arthropod segmentation is still strongly influenced by the huge amount of data available from the fruit fly Drosophila melanogaster (the Drosophila paradigm). However, the simultaneous formation of the segments in Drosophila is a derived mode of segmentation. Successive terminal addition of segments from a posteriorly localized presegmental zone is the ancestral mode of arthropod segmentation. This review focuses on the evolutionary conservation and divergence of the genetic mechanisms of segmentation within arthropods. The more downstream levels of the segmentation gene network (e.g., segment polarity genes) appear to be more conserved than the more upstream levels (gap genes, Notch/Delta signaling). Surprisingly, the basally branched arthropod groups also show similarities to mechanisms used in vertebrate somitogenesis. Furthermore, it has become clear that the activation of pair rule gene orthologs is a key step in the segmentation of all arthropods. Important findings of conserved and diverged aspects of segmentation from the last few years now allow us to draw an evolutionary scenario on how the mechanisms of segmentation could have evolved and led to the present mechanisms seen in various insect groups including dipterans like Drosophila. Developmental Dynamics 236:1379 -1391, 2007.

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“…Recent work on T. castaneum has revealed divergent regulatory interactions between the homologues of D. melanogaster pair-rule genes. Choe et al (2006), show that it is the homologues of D. melanogaster even-skipped, D. melanogaster runt and D. melanogaster odd-skipped that comprise the primary tier of pair-rule genes in T. castaneum. Surprisingly, rather than Localized at the posterior pole is a complex of maternal proteins and RNAs that includes transcripts of the gene nanos.…”

Section: Molecular Transitions Underlying the Evolution Of Long Germ mentioning

confidence: 99%

The evolution of developmental gene networks: lessons from comparative studies on holometabolous insects

Peel

2008

Phil. Trans. R. Soc. B

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Recent comparative studies have revealed significant differences in the developmental gene networks operating in three holometabolous insects: the beetle Tribolium castaneum, the parasitic wasp Nasonia vitripennis and the fruitfly Drosophila melanogaster. I discuss these differences in relation to divergent and convergent changes in cellular embryology. I speculate on how segmentation gene networks have evolved to operate in divergent embryological contexts, and highlight the role that co-option might have played in this process. I argue that insects represent an important example of how diversification in life-history strategies between lineages can lead to divergence in the genetic and cellular mechanisms controlling the development of homologous adult structures.

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A pair-rule gene circuit defines segments sequentially in the short-germ insect Tribolium castaneum

Cited by 196 publications

References 36 publications

Changing cell behaviours during beetle embryogenesis correlates with slowing of segmentation

Changing cell behaviours during beetle embryogenesis correlates with slowing of segmentation

Evolutionary conservation and divergence of the segmentation process in arthropods

The evolution of developmental gene networks: lessons from comparative studies on holometabolous insects

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