A pair of floral regulators sets critical day length for Hd3a florigen expression in rice

Itoh, Hironori; Nonoue, Yasunori; Yano, Masahiro; Izawa, Takeshi

doi:10.1038/ng.606

Cited by 248 publications

(330 citation statements)

References 26 publications

Supporting

Mentioning

309

Contrasting

Unclassified

Order By: Relevance

“…(Supplemental Figures 5A and 5B). We also observed that the expression of Heading date 3a (Hd3a), Early heading date 1 (Ehd1), and RICE FLOWERING LOCUS T1 (RFT1), critical genes known to promote flowering in rice (Doi et al, 2004;Komiya et al, 2009;Itoh et al, 2010), were significantly downregulated in osnrt1.1a mutants ( Figure 6D). Furthermore, the expression of both OsNRT1.1A and Hd3a in the wild type was dramatically repressed by high N treatment, which is in accordance with the late-flowering phenotype caused by high N application (Supplemental Figures 5C to 5E).…”

Section: Loss Of Function Of Osnrt11a Reduces the Utilization Of Botmentioning

confidence: 67%

Expression of the Nitrate Transporter Gene OsNRT1.1A/OsNPF6.3 Confers High Yield and Early Maturation in Rice

et al. 2018

View full text Add to dashboard Cite

Nitrogen (N) is a major driving force for crop yield improvement, but application of high levels of N delays flowering, prolonging maturation and thus increasing the risk of yield losses. Therefore, traits that enable utilization of high levels of N without delaying maturation will be highly desirable for crop breeding. Here, we show that OsNRT1.1A (OsNPF6.3), a member of the rice (Oryza sativa) nitrate transporter 1/peptide transporter family, is involved in regulating N utilization and flowering, providing a target to produce high yield and early maturation simultaneously. OsNRT.1A has functionally diverged from previously reported NRT1.1 genes in plants and functions in upregulating the expression of N utilization-related genes not only for nitrate but also for ammonium, as well as flowering-related genes. Relative to the wild type, osnrt1.1a mutants exhibited reduced N utilization and late flowering. By contrast, overexpression of OsNRT1.1A in rice greatly improved N utilization and grain yield, and maturation time was also significantly shortened. These effects were further confirmed in different rice backgrounds and also in Arabidopsis thaliana. Our study paves a path for the use of a single gene to dramatically increase yield and shorten maturation time for crops, outcomes that promise to substantially increase world food security.

show abstract

Section: Loss Of Function Of Osnrt11a Reduces the Utilization Of Botmentioning

confidence: 67%

Expression of the Nitrate Transporter Gene OsNRT1.1A/OsNPF6.3 Confers High Yield and Early Maturation in Rice

et al. 2018

View full text Add to dashboard Cite

show abstract

“…4c). However, the expression level of Ghd7 (Doi et al 2004;Itoh et al 2010) increased significantly only in Ubi1::OsNF-YC2:GFP plants, suggesting that the OsNF-YC2 protein may also promote the expression of the Ghd7 gene, which then reduces the expression of Ehd1, Hd3a, and RFT1s under LD conditions (Fig. 4a).…”

Section: Osnf-yc Proteins Are Ld-specific Flowering Regulatorsmentioning

confidence: 94%

OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice

Kim

Park

Jang

et al. 2015

Planta

View full text Add to dashboard Cite

Plant NUCLEAR FACTOR Y (NF-Y) transcription factors control numerous developmental processes by forming heterotrimeric complexes, but little is known about their roles in flowering in rice. In this study, it is shown that some subunits of OsNF-YB and OsNF-YC interact with each other, and among them, OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response of rice. Protein interaction studies showed that the physical interactions occurred between the three OsNF-YC proteins (OsNF-YC2, OsNF-YC4 and OsNF-YC6) and three OsNF-YB proteins (OsNF-YB8, OsNF-YB10 and OsNF-YB11).Repression and overexpression of the OsNF-YC2 and OsNF-YC4 genes revealed that they act as inhibitors of flowering only under long-day (LD) conditions. Overexpression of OsNF-YC6, however, promoted flowering only under LD conditions, suggesting it could function as a flowering promoter. These phenotypes correlated with the changes in the expression of three rice flowering-time genes [Early heading date 1 (Ehd1), Heading date 3a (Hd3a) and RICE FLOWERING LOCUS T1 (RFT1)]. The diurnal and tissue-specific expression patterns of the subsets of OsNF-YB and OsNF-YC genes were similar to those of CCT domain encoding genes such as OsCO3, Heading date 1 (Hd1) and Ghd7. We propose that OsNF-YC2 and OsNF-YC4 proteins regulate the photoperiodic flowering response by interacting directly with OsNF-YB8, OsNF-YB10 or OsNF-YB11 proteins in rice.

show abstract

“…In LD conditions Hd1 acts as a flowering repressor inhibiting Hd3a expression but promotes its expression and subsequent flowering in SD [41]. Ghd7 is a small protein with a CCT-domain that represses Ehd1 expression and downstream Hd3a/RFT1 expression in LD conditions to delay flowering [42][43][44][45]. Recent studies demonstrated that the interaction between Ghd7 and Hd1 can play a critical role in repressing Ehd1.…”

Section: Arabidopsis and Ricementioning

confidence: 99%

“…The plant circadian system consists of biochemical timing mechanisms that temporarily modulate the function of several signalling pathways to measures changes in day-length and promote suitable timing of flowering to maximize reproductive success [2,3,6,7,9,10,[13][14][15]21,24,26,28,29,31,36,38,41,42,46,48,49,[56][57][58][59][60][61][62][63][64][65][66]. The photoperiod response on flowering time varies among grasses.…”

Section: Circadian Clock and Photoperiod Responsementioning

confidence: 99%

Molecular Regulation of Flowering Time in Grasses

Nuñez

Yamada

2017

Agronomy

View full text Add to dashboard Cite

Flowering time is a key target trait for extending the vegetative phase to increase biomass in bioenergy crops such as perennial C 4 grasses. Molecular genetic studies allow the identification of genes involved in the control of flowering in different species. Some regulatory factors of the Arabidopsis pathway are conserved in other plant species such as grasses. However, differences in the function of particular genes confer specific responses to flowering. One of the major pathways is photoperiod regulation, based on the interaction of the circadian clock and environmental light signals.Depending on their requirements for day-length plants can be classified as long-day (LD), short-day (SD), and day-neutral. The CONSTANS (CO) and Heading Date 1 (Hd1), orthologos genes, are central regulators in the flowering of Arabidopsis and rice, LD and SD plants, respectively. Additionally, Early heading date 1 (Ehd1) induces the expression of Heading date 3a (Hd3a), conferring SD promotion and controls Rice Flowering Locus T 1 (RFT1) in LD conditions, independently of Hd1. Nevertheless, the mechanisms promoting flowering in perennial bioenergy crops are poorly understood. Recent progress on the regulatory network of important gramineous crops and components involved in flowering control will be discussed.

show abstract

A pair of floral regulators sets critical day length for Hd3a florigen expression in rice

Cited by 248 publications

References 26 publications

Expression of the Nitrate Transporter Gene OsNRT1.1A/OsNPF6.3 Confers High Yield and Early Maturation in Rice

Expression of the Nitrate Transporter Gene OsNRT1.1A/OsNPF6.3 Confers High Yield and Early Maturation in Rice

OsNF-YC2 and OsNF-YC4 proteins inhibit flowering under long-day conditions in rice

Molecular Regulation of Flowering Time in Grasses

Contact Info

Product

Resources

About