A Paeonia ostii caffeoyl-CoA O-methyltransferase confers drought stress tolerance by promoting lignin synthesis and ROS scavenging

Zhao, Daqiu; Luan, Yuting; Shi, Wenbo; Zhang, Xiayan; Meng, Jiasong; Tao, Jun

doi:10.1016/j.plantsci.2020.110765

Cited by 58 publications

(38 citation statements)

References 35 publications

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“…In this study, P n, G s, C i, and T r were markedly increased in TL versus WT. This suggests that PlMYB108 alleviates the DS-mediated suppression of photosynthesis, which is in accordance with results from the P. ostii caffeoyl-CoA O -methyltransferase overexpression examinations [ 30 ]. In addition, chlorophyll fluorescence parameters are used as internal indices of plants’ adaptation to the eco-environment, and their changes during environmental stress can be used to judge the degree of damage to photosynthetic organs.…”

Section: Discussionsupporting

confidence: 89%

R2R3-MYB Transcription Factor PlMYB108 Confers Drought Tolerance in Herbaceous Peony (Paeonia lactiflora Pall.)

Cheng

et al. 2021

IJMS

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The MYB transcription factor (TF) is crucial for plant growth, development, and response to abiotic stress, but it is rarely reported in the herbaceous peony (Paeonia lactiflora Pall.). Here, an MYB TF gene was isolated, and based on our prior mRNA data from P. lactiflora samples, it was treated with drought stress (DS). Its complete cDNA structure was 1314 bp, which encoded 291 amino acids (aa). Furthermore, using sequence alignment analysis, we demonstrated that PlMYB108 was an R2R3-MYB TF. We also revealed that PlMYB108 was primarily localized in the nucleus. Its levels rose during DS, and it was positively correlated with drought tolerance (DT) in P. lactiflora. In addition, when PlMYB108 was overexpressed in tobacco plants, the flavonoid content, antioxidant enzyme activities, and photosynthesis were markedly elevated. Hence, the transgenic plants had stronger DT with a higher leaf water content and lower H2O2 accumulation compared to the wild-type (WT) plants. Based on these results, PlMYB108 is a vital gene that serves to increase flavonoid accumulation, reactive oxygen species (ROS), scavenging capacity, and photosynthesis to confer DT. The results would provide a genetic resource for molecular breeding to enhance plant DT.

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Section: Discussionsupporting

confidence: 89%

R2R3-MYB Transcription Factor PlMYB108 Confers Drought Tolerance in Herbaceous Peony (Paeonia lactiflora Pall.)

Cheng

et al. 2021

IJMS

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“…The plant cell wall plays a huge role in protecting cells against a wide variety of biotic and abiotic stresses [ 50 , 51 ]. The phenolic cross-linking between lignin and hemicellulose adds strength to cell walls [ 32 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

The Photosynthetic Efficiency and Carbohydrates Responses of Six Edamame (Glycine max. L. Merrill) Cultivars under Drought Stress

Hlahla

Mafa

Merwe

et al. 2022

Plants

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Vegetable-type soybean, also known as edamame, was recently introduced to South Africa. However, there is lack of information on its responses to drought. The aim of this study was to investigate the photosynthetic efficiency and carbohydrates responses of six edamame cultivars under drought stress. Photosynthetic efficiency parameters, including chlorophyll fluorescence and stomatal conductance, were determined using non-invasive methods, while pigments were quantified spectrophotometrically. Non-structural carbohydrates were quantified using Megazyme kits. Structural carbohydrates were determined using Fourier-transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). Drought stress significantly increased the Fv/Fm and PIabs of AGS429 and UVE17 at pod filling stage. Chlorophyll-a, which was most sensitive to drought, was significantly reduced in AGS429 and UVE17, but chlorophyll-b was relatively stable in all cultivars, except UVE17, which showed a significant decline at flowering stage. AGS354 and AGS429 also showed reduced chlorophyll-b at pod filling. UVE17 showed a significant reduction in carotenoid content and a substantial reduction in stomatal conductance during pod filling. Drought stress during pod filling resulted in a significant increase in the contents of trehalose, sucrose and starch, but glucose was decreased. Chlorophyll-a positively correlated with starch. The FTIR and XRD results suggest that the cell wall of UVE14, followed by UVE8 and AGS429, was the most intact during drought stress. It was concluded that carotenoids, stomatal conductance, starch and hemicellulose could be used as physiological/biochemical indicators of drought tolerance in edamame. This information expands our knowledge of the drought defense responses in edamame, and it is essential for the physiological and biochemical screening of drought tolerance.

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“…Several studies have shown that the process of lignification is important for drought tolerance. Overexpression of IbLEA14 in sweet potato (Park et al, 2011), OsTF1L in rice (Bang et al, 2019), VlbZIP30 in grapevine (Tu et al, 2020), and PoCCoAOMT in tobacco (Zhao et al, 2021) all enhanced lignin biosynthesis and drought resistance. In maize, drought-tolerant inbred lines showed higher lignification than drought-sensitive lines, suggesting that lignification is an important adaptation to drought stress (Hu et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of the HD-ZIP III Subfamily in Upland Cotton Reveals the Involvement of GhHB8-5D in the Biosynthesis of Secondary Wall in Fiber and Drought Resistance

et al. 2022

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A subfamily of transcription factors known as HD-ZIP III plays distinct roles in the secondary cell wall biosynthesis, which could be attributed to the quality of cotton fiber and adaptation to drought stress. In this study, 18 HD-ZIP III genes were identified as genome wide from the upland cotton (Gossypium hirsutum). These genes are distributed on 14 different chromosomes, and all of them have undergone segmental duplications. Numerous cis-elements were identified in the promoter regions, which are related to phytohormone responses and abiotic stresses. Expression profiling of these genes by quantitative real-time (qRT)-PCR illustrated their differential spatial expression, with preferential expression in cotton fiber. Among these genes, GhHB8-5D was predicted to encode a protein that is targeted to the cell nucleus and having self-activation ability. In addition, the ectopic expression of GhHB8-5D or its synonymous mutant GhHB8-5Dm in Arabidopsis resulted in stunted plant growth, curly leaves, and twisted inflorescence stems. Microscopy examination revealed that the morphology of vascular bundles and deposition of secondary wall had substantially altered in stems, which is concomitant with the significant alteration in the transcription levels of secondary wall-related genes in these transgenic Arabidopsis. Further, ectopic expression of GhHB8-5D or GhHB8-5Dm in Arabidopsis also led to significant increase in green seedling rate and reduction in root length relative to wild type when the plants were grown under mimicked drought stress conditions. Taken together, our results may shed new light on the functional roles of GhHB8-5D that is attributable for secondary cell wall thickening in response to drought stress. Such a finding may facilitate a novel strategy for improving plant adaptations to environmental changes via regulating the biosynthesis of secondary cell wall.

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A Paeonia ostii caffeoyl-CoA O-methyltransferase confers drought stress tolerance by promoting lignin synthesis and ROS scavenging

Cited by 58 publications

References 35 publications

R2R3-MYB Transcription Factor PlMYB108 Confers Drought Tolerance in Herbaceous Peony (Paeonia lactiflora Pall.)

R2R3-MYB Transcription Factor PlMYB108 Confers Drought Tolerance in Herbaceous Peony (Paeonia lactiflora Pall.)

The Photosynthetic Efficiency and Carbohydrates Responses of Six Edamame (Glycine max. L. Merrill) Cultivars under Drought Stress

Genome-Wide Identification of the HD-ZIP III Subfamily in Upland Cotton Reveals the Involvement of GhHB8-5D in the Biosynthesis of Secondary Wall in Fiber and Drought Resistance

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