A P4-ATPase Gene GbPATP of Cotton Confers Chilling Tolerance in Plants

Liu, Tingli; Guo, Shiwei; Lian, Ziyi; Chen, Fei; Yang, Youxin; Chen, Tianzi; Ling, Xitie; Liu, Aiming; Wang, Rongfu; Zhang, Baolong

doi:10.1093/pcp/pcu200

Cited by 21 publications

(15 citation statements)

References 39 publications

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“…Particularly, if exposed to low temperature in the period of germination or seedling, cotton growth and development would be slow and at the time of budding, flowering and maturity would be delayed (Gai et al 2008). In addition, low temperature not only affects the yield of cotton but also the quality of fibre (Liu et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…The species is highly tolerant to low temperature and can survive low temperature as low as −6 °C (Liang et al 1996). Researchers have identified some significant genes related to chilling tolerance in cotton, such as GhDREB1 (Shan et al 2007), GhTIP1 (Li et al 2009), GhMT3a (Xue et al 2009), phospholipase Dα (Kargiotidou et al 2010), GbCBF1 (Guo et al 2011), GbPATP (Liu et al 2015) and GhTPS11 (Wang et al 2016). The overexpression of DREB/CBF genes has been found to confer cold tolerance to transgenic plants (Kang et al 2013).…”

Section: Introductionmentioning

confidence: 99%

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Comparative transcriptome, physiological and biochemical analyses reveal response mechanism mediated by CBF4 and ICE2 in enhancing cold stress tolerance in Gossypium thurberi

Cai

Magwanga

et al. 2019

AoB PLANTS

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Low temperature is one of the key environmental stresses that impair plant growth and significantly restricts the productivity and spatial distribution of crop plants. Gossypium thurberi, a wild diploid cotton species, has adapted to a wide range of temperatures and exhibits a better tolerance to chilling stress. Here, we compared phenotypes and physiochemical changes in G. thurberi under cold stress and found this species indeed showed better cold tolerance. Therefore, to understand the molecular mechanisms of the cold tolerance in G. thurberi, we compared transcription changes in leaves of G. thurberi under cold stress by high-throughput transcriptome sequencing. In total, 35 617 unigenes were identified in the whole-genome transcription profile, and 4226 differentially expressed genes (DEGs) were discovered in the leaves upon cold treatment. Gene Ontology (GO) classification analyses showed that the majority of DEGs belonged to categories of signal transduction, transcription factors (TFs) and carbohydrate transport and metabolism. The expression of several cold-responsive genes such as ICE1, CBF4, RAP2-7 and abscisic acid (ABA) biosynthesis genes involved in different signalling pathways were induced after G. thurberi seedlings were exposed to cold stress. Furthermore, cold sensitivity was increased in CBF4 and ICE2 virus-induced gene silencing (VIGS) plants, and high level of malondialdehyde (MDA) showed that the CBF4 and ICE2 silenced plants were under oxidative stress compared to their wild types, which relatively had higher levels of antioxidant enzyme activity, as evident by high levels of proline and superoxide dismutase (SOD) content. In conclusion, our findings reveal a new regulatory network of cold stress response in G. thurberi and broaden our understanding of the cold tolerance mechanism in cotton, which might accelerate functional genomics studies and genetic improvement for cold stress tolerance in cultivated cotton.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome, physiological and biochemical analyses reveal response mechanism mediated by CBF4 and ICE2 in enhancing cold stress tolerance in Gossypium thurberi

Cai

Magwanga

et al. 2019

AoB PLANTS

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“…The expression of GhMYB36 in the Agro‐infiltrated G . hirsutum plants was determined by qRT‐PCR using MYB36F and MYB36R as primers, and cotton ubiquitin 14 (UBQ14) as a reference gene (Liu et al ., 2014a ).…”

Section: Methodsmentioning

confidence: 99%

“…Agro-infiltration for VIGS in cotton was performed by plants following the procedures as previously described (Gu et al, 2014;Zhang et al, 2012). The expression of GhMYB36 in the Agro-infiltrated G. hirsutum plants was determined by qRT-PCR using MYB36F and MYB36R as primers, and cotton ubiquitin 14 (UBQ14) as a reference gene (Liu et al, 2014a).…”

Section: Virus-induced Gene Silencing In Cottonmentioning

confidence: 99%

The GhMYB36 transcription factor confers resistance to biotic and abiotic stress by enhancing PR1 gene expression in plants

Liu

Chen

Kan

et al. 2021

Plant Biotechnology Journal

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Drought and Verticillium wilt disease are two main factors that limit cotton production, which necessitates the identification of key molecular switch to simultaneously improve cotton resistance to Verticillium dahliae and tolerance to drought stress. R2R3-type MYB proteins could play such a role because of their conserved functions in plant development, growth, and metabolism regulation, however, till date a MYB gene conferring the desired resistance to both biotic and abiotic stresses has not been found in cotton. Here, we describe the identification of GhMYB36, a gene encoding a R2R3-type MYB protein in Gossypium hirsutum, which confers drought tolerance and Verticilium wilt resistance in both Arabidopsis and cotton. GhMYB36 was highly induced by PEG-simulated drought stress in G. hirsutum. GhMYB36-silenced cotton plants were more sensitive to both drought stress and Verticillium wilt. GhMYB36 overexpression in transgenic Arabidopsis and cotton plants gave rise to improved drought tolerance and Verticillium wilt resistance. Transient expression of fused GhMYB36-GFP in tobacco cells was able to localize GhMYB36 in the cell nucleus. In addition, RNA-seq analysis together with qRT-PCR validation in transgenic Arabidopsis overexpressing GhMYB36 revealed significantly enhanced PR1 expression. Luciferase interaction assays indicated that GhMYB36 are probably bound to the promoter of PR1 to activate its expression and the interaction, which was further verified by Yeast one hybrid assay. Taken together, our results suggest that GhMYB36 functions as a transcription factor that is involved in drought tolerance and Verticillium wilt resistance in Arabidopsis and cotton by enhancing PR1 expression.

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“…Our development of a cotton leaf crumple virus (CLCrV)-based virus-induced gene silencing (VIGS) vector has been proven to be an efficient gene-silencing tool that can be used for reverse genetics and functional analyses of candidate genes in cotton (Zhang et al, 2012 ; Gu et al, 2014 ; Liu et al, 2014 , 2015 ; Chen et al, 2015 ; Fu et al, 2015 ; Zhang H. et al, 2016 ; Zhang W. et al, 2016 ). In this study, we employed CLCrV-based VIGS methods to study the function of two members of the cotton LYK family, Gh-LYK1 and Gh-LYK2 , in defense.…”

Section: Introductionmentioning

confidence: 99%

Two Lysin-Motif Receptor Kinases, Gh-LYK1 and Gh-LYK2, Contribute to Resistance against Verticillium wilt in Upland Cotton

Liu

Ding

et al. 2017

Front. Plant Sci.

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Lysin-motif (LysM) receptor kinases (LYKs) play essential roles in recognition of chitin and activation of defense responses against pathogenic fungi in the model plants Arabidopsis and rice. The function of LYKs in non-model plants, however, remains elusive. In the present work, we found that the transcription of two LYK-encoding genes from cotton, Gh-LYK1 and Gh-LYK2, was induced after Verticillium dahliae infection. Virus-induced gene silencing (VIGS) of Gh-LYK1 and Gh-LYK2 in cotton plants compromises resistance to V. dahliae. As putative pattern recognition receptors (PRRs), both Gh-LYK1 and Gh-LYK2 are membrane-localized, and all three LysM domains of Gh-LYK1 and Gh-LYK2 are required for their chitin-binding ability. However, since Gh-LYK2, but not Gh-LYK1, is a pseudo-kinase and, on the other hand, the ectodomain (ED) of Gh-LYK2 can induce reactive oxygen species (ROS) burst in planta, Gh-LYK2 and Gh-LYK1 may contribute differently to cotton defense. Taken together, our results establish that both Gh-LYK1 and Gh-LYK12 are required for defense against V. dahliae in cotton, possibly through different mechanisms.

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A P4-ATPase Gene GbPATP of Cotton Confers Chilling Tolerance in Plants

Cited by 21 publications

References 39 publications

Comparative transcriptome, physiological and biochemical analyses reveal response mechanism mediated by CBF4 and ICE2 in enhancing cold stress tolerance in Gossypium thurberi

Comparative transcriptome, physiological and biochemical analyses reveal response mechanism mediated by CBF4 and ICE2 in enhancing cold stress tolerance in Gossypium thurberi

The GhMYB36 transcription factor confers resistance to biotic and abiotic stress by enhancing PR1 gene expression in plants

Two Lysin-Motif Receptor Kinases, Gh-LYK1 and Gh-LYK2, Contribute to Resistance against Verticillium wilt in Upland Cotton

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