A one-tube plasmid DNA mini-preparation suitable for sequencing

Sal, Giannino Del; Manfioletti, Guidalberto; Schneider, Claudio

doi:10.1093/nar/16.20.9878

Cited by 257 publications

(100 citation statements)

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“…Small-and largescale preparations of plasmid DNA were made with the cetyltrimethylammonium bromide method (Del Sal et al, 1988) and the Jetstar kit (Genomed), respectively. DNA fragments were purified from agarose gels with the Geneclean II kit (Bio 101) or the MinElute Gel Extraction Kit (Qiagen).…”

Section: Methodsmentioning

confidence: 99%

Ferripyochelin uptake genes are involved in pyochelin-mediated signalling in Pseudomonas aeruginosa

2007

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In response to iron starvation, Pseudomonas aeruginosa produces the siderophore pyochelin. When secreted to the extracellular environment, pyochelin chelates iron and transports it to the bacterial cytoplasm via its specific outer-membrane receptor FptA and the inner-membrane permease FptX. Exogenously added pyochelin also acts as a signal which induces the expression of the pyochelin biosynthesis and uptake genes by activating PchR, a cytoplasmic regulatory protein of the AraC/XylS family. The importance of ferripyochelin uptake genes in this regulation was evaluated. The fptA and fptX genes were shown to be part of the fptABCX ferripyochelin transport operon, which is conserved in Burkholderia sp. and Rhodospirillum rubrum. The fptB and fptC genes were found to be dispensable for utilization of pyochelin as an iron source, for signalling and for pyochelin production. By contrast, mutations in fptA and fptX not only interfered with pyochelin utilization, but also affected signalling and diminished siderophore production. It is concluded from this that pyochelin-mediated signalling operates to a large extent via the ferripyochelin transport system.

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Section: Methodsmentioning

confidence: 99%

Ferripyochelin uptake genes are involved in pyochelin-mediated signalling in Pseudomonas aeruginosa

2007

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“…DNA bands were collected and dye-extracted with 1-butanol prior to precipitation (Sambrook et al, 1989). Plasmid DNA was prepared by the boiling method (Del Sal et al, 1988). λ DNA was obtained as described by Grossberger (1987).…”

Section: Methodsmentioning

confidence: 99%

Comparison of ribitol and glycerol teichoic acid genes in Bacillus subtilis W23 and 168: identical function, similar divergent organization, but different regulation The EMBL accession numbers for the nucleotide sequences reported in this paper are AJ313428, AJ318465, AJ318466, AJ318467, AJ318468, AJ318469 and AJ318470.

et al. 2002

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The tar genes directing the synthesis of poly(ribitol phosphate), the main teichoic acid in Bacillus subtilis strain W23, were sequenced. They are organized in two divergently transcribed operons, tarABIJKL and tarDF, as are the tag genes specifying poly(glycerol phosphate) synthesis in B. subtilis 168. The features of the tar genes as well as the putative participation of their products in the proposed biosynthesis pathway of poly(ribitol phosphate) are presented. The tarA and tarD genes, which are most likely involved in the synthesis of the linkage unit (the entity coupling teichoic acid to peptidoglycan), are separated by 508 nt. Sequences of the outer segments of this regulatory region are similar to the two divergent promoter regions identified upstream of the tagA and tagD genes in strain 168. However, in W23, these regions, which also included functional promoters, are separated by an additional DNA segment of about 100 nt, on which two new mRNA starts, one in each direction, were identified. The regulatory regions of teichoic acid divergons of Bacillus globigii, Bacillus licheniformis and eight strains of B. subtilis were cloned and sequenced. In four B. subtilis strains and in B. globigii, their length and sequence are similar to the regulatory region of W23. In the others, including B. licheniformis, they are of the 168-type. Analysis of nucleotide sequences of a non-coding grey hole, present in the tag region of strain 168, revealed higher similarities to tar than to tag entities. This suggests that at least part of the tag genes specifying the synthesis of glucosylated poly(glycerol phosphate) in strain 168 was introduced by horizontal gene transfer into a strain originally synthesizing a ribitol-phosphate-containing teichoic acid.

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“…PstI and PstI-AvaI fragments were subcloned into pUC 19 by standard ligation and transformation techniques, using Escherichia coli host strain DH 5a. Plasmid DNA was isolated by the method of Sal et al [21]. Copy number of truncated Bov-B LINE elements in K ammodytes genome.…”

Section: Methodsmentioning

confidence: 99%

Bov‐B Long Interspersed Repeated DNA (LINE) Sequences are Present in Vipera Ammodytes Phospholipase A₂ Genes and in Genomes of Viperidae Snakes

Kordiš

Gubenšek

1997

European Journal of Biochemistry

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Ammodytin L is a myotoxic Ser49 phospholipase A, (PLA,) homologue, which is tissue-specifically expressed in the venom glands of Vipera ammodytes. The complete DNA sequence of the gene and its 5' and 3' flanking regions has been determined. The gene consists of five exons separated by four introns. Comparative analysis of the ammodytin L and ammodytoxin C genes shows that all intron and flanking sequences are considerably more conserved (93-97 %) than the mature protein-coding exons. The pattern of nucleotide substitutions in protein-coding exons is not random but occurs preferentially on the first and the second positions of codons, which suggests positive Darwinian evolution for a new function. An Ruminantia specific ART-2 retroposon, recently recognised as a 5'-truncated Bov-B long interspersed repeated DNA (LINE) sequence, was identified in the fourth intron of both genes. This result suggests that ammodytin L and ammodytoxin C genes are derived by duplication of a common ancestral gene.

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A one-tube plasmid DNA mini-preparation suitable for sequencing

Cited by 257 publications

References 5 publications

Ferripyochelin uptake genes are involved in pyochelin-mediated signalling in Pseudomonas aeruginosa

Ferripyochelin uptake genes are involved in pyochelin-mediated signalling in Pseudomonas aeruginosa

Bov‐B Long Interspersed Repeated DNA (LINE) Sequences are Present in Vipera Ammodytes Phospholipase A₂ Genes and in Genomes of Viperidae Snakes

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A one-tube plasmid DNA mini-preparation suitable for sequencing

Cited by 257 publications

References 5 publications

Ferripyochelin uptake genes are involved in pyochelin-mediated signalling in Pseudomonas aeruginosa

Ferripyochelin uptake genes are involved in pyochelin-mediated signalling in Pseudomonas aeruginosa

Bov‐B Long Interspersed Repeated DNA (LINE) Sequences are Present in Vipera Ammodytes Phospholipase A2 Genes and in Genomes of Viperidae Snakes

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Bov‐B Long Interspersed Repeated DNA (LINE) Sequences are Present in Vipera Ammodytes Phospholipase A₂ Genes and in Genomes of Viperidae Snakes