A one step sandwich enzyme immunoassay for γ-carboxylated osteocalcin using monoclonal antibodies

Uchiyama

et al. 2006

The effects of prolonged intake of juice prepared from Satsuma mandarin (Citrus unshiu MARC.) containing β-cryptoxanthin on circulating biochemical markers of bone metabolism in subjects, including menopausal woman, were investigated. Ninety volunteers, aged 27-65 years (19 men and 71 women), were enrolled in this study. The 71 females included 35 premenopausal women (ages, 27-50 years) and 36 postmenopausal women (ages, 46-65 years). Volunteers were divided into four groups; placebo juice without β-cyptoxanthin (5 men and 19 women), juice containing β-cyptoxanthin at 1.5 mg/200 ml of juice/day (4 men and 17 women), 3.0 mg/day (5 men and 17 women), and 6.0 mg/day (5 men and 18 women). Placebo or juice (200 ml) was ingested once a day for 28 or 56 days. Serum β-cryptoxanthin concentrations were significantly increased after the intake of juice containing β-cryptoxanthin (1.5, 3.0, or 6.0 mg/day) for 28 or 56 days, and the increases were dose-dependent. Bone-specific alkaline phosphatase and γ-carboxylated osteocalcin are serum bone markers of bone formation, and bone tartrate-resistant acid phosphatase (TRACP) and N-telopeptides of type I collagen are markers of bone resorption. Bone-specific alkaline phosphatase activity was significantly increased after the intake of juice containing β-cryptoxanthin (3.0 or 6.0 mg/ day) for 56 days as compared with the value obtained before intake. γ-Carboxylated osteocalcin concentration was significantly increased after the intake of juice containing β-cryptoxanthin (3.0 or 6.0 mg/day) for 28 or 56 days as compared with the value obtained before intake or after the intake of placebo juice. Serum TRACP activity and type I collagen N-telopeptide concentration were significantly decreased after the intake of juice containing β-cryptoxanthin (3.0 or 6.0 mg/day) for 28 or 56 days as compared with the value obtained before intake or after intake of placebo juice, and significant decreases were also seen after the intake of 1.5 mg/day β-cryptoxanthin as compared with the value obtained before intake. In menopausal women, bone-specific alkaline phosphatase activity and γ-carboxylated osteocalcin concentration were significantly increased after the intake of juice containing β-cryptoxanthin (3.0 or 6.0 mg/day) for 56 days as compared with the value obtained after placebo intake. Also, this intake caused a significant decrease in bone TRACP activity. Meanwhile, serum calcium, inorganic phosphorous, and parathyroid hormone (intact) were not changed after the intake of β-cryptoxanthin-containing juice for 28 or 56 days. This study demonstrates that the prolonged intake of juice fortified with β-cryptoxanthin has stimulatory effects on bone formation and inhibitory effects on bone resorption in humans, and that the intake has an effect in menopausal women.

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Section: Methodsmentioning

confidence: 99%

“…β-Cryptoxanthin has been demonstrated to stimulate cell proliferation and mineralization in osteoblastic cells in vitro. 19,20) Serum bone TRACP is a specific marker enzyme in osteoclasts, 28) and N-telopeptide of type I collagen is specifically formed following the stimulation of bone resorption.…”

Section: )mentioning

confidence: 99%

Effect of .BETA.-Crytoxanthin on Circulating Bone Metabolic Markers: Intake of Juice (Citrus Unshiu) Supplemented with .BETA.-Cryptoxanthin Has an Effect in Menopausal Women

Uchiyama

et al. 2006

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“…The serum γ-carboxylated osteocalcin concentration was assayed using a Gla-type Osteocalcin (Gla-OC) EIA kit (Takara Shuzou, Shiga, Japan). 24) Serum bone-specific alkaline phosphatase activity was assayed using a Metra BAP EIA kit (Quidel, San Diego, CA, U.S.A.). 25) Serum bone tartrate-resistant acid phosphatase (TRAP) activity was assayed using a Bone TRAP Assay EIA kit (SBA Sciences, Turku, Finland).…”

Section: Methodsmentioning

confidence: 99%

Prolonged Intake of Dietary Fermented Isoflavone-Rich Soybean Reinforced with Zinc Affects Circulating Bone Biochemical Markers in Aged Individuals

Sakai

et al. 2005

“…28) Serum bone tartrate-resistant acid phosphatase (TRAP) activity was assayed using the Bone TRAP Assay EIA kit (SBA Sciences, Turku, Finland). 29) Serum albumin, γ-GTP, nitrogen urea, amylase, glucose, high-density lipoprotein (HDL) cholesterol, triglyceride, and zinc levels were determined using kits (Wako Pure Chemicals, Osaka, Japan).…”

Section: Methodsmentioning

confidence: 99%

Relationship between Serum β-Cryptoxanthin and Circulating Bone Metabolic Markers in Healthy Individuals with the Intake of Juice (Citrus unshiu) Containing β-Cryptoxanthin

Morita³

et al. 2005