A One-Step Immunostaining Method to Visualize Rodent Muscle Fiber Type within a Single Specimen

Sawano, Shoko; Komiya, Yusuke; Ichitsubo, Riho; Ohkawa, Yasuyuki; Nakamura, Mako; Tatsumi, Ryuichi; Ikeuchi, Yoshihide; Mizunoya, Wataru

doi:10.1371/journal.pone.0166080

Cited by 48 publications

(41 citation statements)

References 41 publications

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“…Muscle is composed of two types of muscle fibers, slow and fast, which have different metabolic characteristics, and these fibers are heterogeneously localized in the muscle. In gastrocnemius, we could divide it into three portions depends on the different ratio of these fibers 3 . Muscle atrophy and hypertrophy have a tendency to occur predominantly in fast fibers.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Lipid Changes That Occur in Hypertrophic Muscle due to Fish Protein-feeding Using Mass Spectrometry Imaging

et al. 2019

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Alaska pollack protein (APP) was previously shown to reduce serum triacylglycerol and the atherogenic index and significantly increase gastrocnemius muscle mass in rats. To determine which myofibers are involved in this observed hypertrophy, we stained the gastrocnemius muscle with fast and slow fiber-specific antibodies and measured the muscle fiber diameter. We observed muscle hypertrophy in both the fast and slow fibers of APP-fed rats. Although muscle hypertrophy leads to drastic lipid changes, the amount of lipids did not differ significantly between casein-fed and APP-fed rats. To determine the lipid changes at the molecular species level and their localization, we performed matrix-assisted laser desorption/ ionization mass spectrometry imaging to visualize lipids in the gastrocnemius muscles. We determined that lipid molecules were significantly changed due to APP feeding. Thus, APP feeding changes muscle lipid metabolism, and these metabolic changes might be related to hypertrophy.

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Section: Introductionmentioning

confidence: 99%

Investigation of the Lipid Changes That Occur in Hypertrophic Muscle due to Fish Protein-feeding Using Mass Spectrometry Imaging

et al. 2019

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“…The specimens were rapidly frozen in isopentane in liquid nitrogen. The tissue sections were prepared with a cryostat and immunohistochemically stained with an anti-MyHC antibodies provided by the Faculty of Agriculture of Kyushu University 10) .…”

Section: Immunohistochemical Stainingmentioning

confidence: 99%

Masseter Muscle Properties Differ between the Left and Right Sides in Mandibular Class III Patients with Asymmetry

Nakashima

Yamada

Sugiyama

et al. 2020

J. Hard Tissue Biology.

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In this study on muscle characteristics and jawbone morphology, the relationship between masticatory muscle properties and jaw deformity was analysed in skeletal Class III patients with asymmetry. The subjects were 27 patients (21 women and 6 men) who had skeletal mandibular prognathism with mandibular asymmetry. The subjects' ages ranged from 16 to 51 years, with an average of 23.5 years. Patients with lateral displacement of Me 3 mm or more and an ANB angle of 0 ° or less were included. Morphometric measurements were taken from CT images. Biopsy material was collected during surgery for immunohistological typing of muscle fibres, MyHC1, 2a, and 2x isoforms; measurement of MYH1, 2, 7, 8 mRNA expression. In CT morphometry, there was no difference in muscle thickness or cross-sectional area between the deviated and non-deviated sides, but both sides tended to have thinner muscles than the control group. In the typing of muscle fibres by immunohistochemical staining, type 2 fibres were found to be significantly more numerous on the deviated side than on the non-deviated side. Regarding protein quantities, MyHC1 was more abundant on the deviated side than on the non-deviated side. Regarding the expression of mRNA, MYH7 (type 1) and MYH1 (type 2x) showed no difference between the two sides, and MYH2 (type 2a) had a higher expression level on the deviated side than in the control group. The data suggested a tendency for Class III patients to have characteristics of a short face on the deviated side, and a long face on the non-deviated side. It seems that muscle type switching may be in progress.

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“…Classically, myofiber characteristics are determined based on a visual assessment of MyHC expression, and are often categorized on basis of the expression of a single and most dominant isoform only (7,(9)(10)(11). In addition, visual assessments are subjective the number of myofibers that can be scored is limited.…”

Section: Introductionmentioning

confidence: 99%

High-throughput data-driven analysis of myofiber composition reveals muscle-specific disease and age-associated patterns

Raz

Vijver

et al. 2018

Preprint

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Short running title: Data-driven analysis of myofiber compositionNonstandard abbreviations LGMD Limb girdle muscular dystrophy MFI mean fluorescence intensity MyHC Myosin heavy chain SGCA-null α-sarcoglycan-null SGCD-null δ-sarcoglycan-null Abstract Contractile properties of myofibers are dictated by the abundance of myosin heavy chain (MyHC)isoforms. MyHC composition designates muscle function and its alterations could unravel differential muscle involvement in muscular dystrophies and aging. Current analyses are limited to visual assessments in which myofibers expressing multiple MyHC isoforms are prone to misclassifications.As a result, complex patterns and subtle alterations are unidentified. We developed a high-throughputdata-driven myofiber analysis to quantitatively describe the variations in myofibers across the muscle.We investigated alterations in myofiber composition between genotypes, two muscles and two age groups. We show that this analysis facilitates the discovery of complex myofiber compositions and its dependency on age, muscle type and genetic conditions.

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A One-Step Immunostaining Method to Visualize Rodent Muscle Fiber Type within a Single Specimen

Cited by 48 publications

References 41 publications

Investigation of the Lipid Changes That Occur in Hypertrophic Muscle due to Fish Protein-feeding Using Mass Spectrometry Imaging

Investigation of the Lipid Changes That Occur in Hypertrophic Muscle due to Fish Protein-feeding Using Mass Spectrometry Imaging

Masseter Muscle Properties Differ between the Left and Right Sides in Mandibular Class III Patients with Asymmetry

High-throughput data-driven analysis of myofiber composition reveals muscle-specific disease and age-associated patterns

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