A one-step enzyme immunoassay for the determination of total tissue-type plasminogen activator (t-PA) antigen in plasma

Bos, R.; Nobel, Edmund; Laterveer, R.; Meyer, Pascale; Nieuwenhuizen, W.

doi:10.1097/00001721-199206000-00010

Cited by 8 publications

(6 citation statements)

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“…Fibrinogen wasdeterminedbyenzymelinked immunosorbent assay( ELISA; Organon Teknika, Turnhout, Belgium)asdescribed by Hoegee-de Nobel,etal. 22Determination of levels of tPAantigen wascarried outb yELISA (Organon Teknika, Turnhout, Belgium)asdescribed by Bos,et al ( 23). vWF wasa ssessedb yE LISA, using polyclonal antibodies (Dako;Cat.No.…”

Section: Bloodsamplingand Biochemicalassaysmentioning

confidence: 99%

Genetic influences on fibrinogen, tissue plasminogen activatorantigen and von Willebrand factor in males and females

Lange¹,

Geus²,

Kluft³

et al. 2006

Thromb Haemost

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SummaryDifferencesi ng enetic influence on deathf romC HD between males and females have been reported. Haemostatic factors have consistentlybeen associated with riskf or coronaryheart disease (CHD), butsex differencesingenetic architecturehave not been studied.This study in middle-agedt wins investigates whetherthere aresex differencesinmeans and in genetic and/ or environmental variance components of haemostatic riskfactors forCHD.Atotal of 93 monozygotic twin pairs (44 maleand 49 female) and 116dizygotic twin pairs(36 male, 40 femaleand 40 opposite sex) were availablef or this study.S tructural equationmodellingwas usedtoestimatethe relativeinfluence of genetic and environmental factors on variation in levels of fi- KeywordsTw ins, haemostasis,heritability, sexdifferences, oralcontraceptivepill brinogen,t issuep lasminogen activator (tPA)a ntigen and von Willebrand factor (vWF). MeanlevelsoftPA and vWFincreased with age.Oral contraceptivepill (OCP) andmenopause had significanti nfluenceso nl evelso ff ibrinogen and tPA. Genetic influencesexplained 39, 66 and 72% of the variation in levels of fibrinogen,tPA and vWF, respectively. No quantitativeo rq ualitatived ifferenceso fg enetic influenceso nh aemostatic levels were seen between males and females.Haemostatic factors may account forasignificant partofthe genetic risk forcardiovascular disease.Nodifference in genetic architecturefor levels of fibrinogen,t PA or vWFw as observed between males and females.

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Section: Bloodsamplingand Biochemicalassaysmentioning

confidence: 99%

Genetic influences on fibrinogen, tissue plasminogen activatorantigen and von Willebrand factor in males and females

Lange¹,

Geus²,

Kluft³

et al. 2006

Thromb Haemost

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“…Production of uPA and tPA in conditioned medium of cultured synovial fibroblasts was measured by ELISA (18,19). To compare ATF and ATF.BPTI expression, another uPA-specific ELISA recognizing the ATF fragment of uPA was performed in the conditioned medium of ATF-or ATF.BPTI-transduced rheumatoid synovial fibroblasts (11).…”

Section: Adenoviral Vectorsmentioning

confidence: 99%

Cartilage degradation and invasion by rheumatoid synovial fibroblasts is inhibited by gene transfer of a cell surface-targeted plasmin inhibitor

Laan

Pap²,

Ronday

et al. 2000

Arthritis & Rheumatism

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Objective. Joint destruction in rheumatoid arthritis (RA) is a result of degradation and invasion of the articular cartilage by the pannus tissue. The present study was undertaken to examine the role of the plasminogen activation system in cartilage degradation and invasion by synovial fibroblasts and investigate a novel gene therapeutic approach using a cell surface-targeted plasmin inhibitor (ATF.BPTI).Methods. Adenoviral vectors were used for gene transfer. The effects of ATF.BPTI gene transfer on RA synovial fibroblast-dependent cartilage degradation were studied in vitro, and cartilage invasion was studied in vivo in the SCID mouse coimplantation model. Conclusion. These results indicate a role of the plasminogen activation system in synovial fibroblastdependent cartilage degradation and invasion in RA, and demonstrate an effective way to inhibit this by gene transfer of a cell surface-targeted plasmin inhibitor.

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“…blood), kept on ice during the experiment, centrifugated (10 min 2000 X g), and plasma was frozen to -20o C until analysis. Rat endogenous t-PA concenffations were measured using an ELISA specific for rat t-PA (23), and recombinant t-PA was measured using an ELISA specific for human t-PA (24).…”

Section: The Effect Of Dextran Infusion In Vivo In the Ratmentioning

confidence: 99%

Inhibition of Mannose Receptor-mediated Clearance of Tissue-type Plasminogen Activator (t-PA) by Dextran: a New Explanation for Its Antithrombotic Effect

Noorman¹,

Barrett-Bergshoeff²,

Bekkers³

et al. 1997

Thromb Haemost

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SummaryDextran is used during surgery as a prophylactic agent to prevent deep venous thrombosis. Recently it has been shown that dextran increases t-PA plasma concentrations in patients. As dextran is a potential ligand for the mannose receptor, we studied whether this glucose-polymer would be able to inhibit mannose receptor-mediated clearance of t-PA.In this report we show that dextran 40 and dextran 70 were able to inhibit t-PA binding to the isolated human mannose receptor (IC5014 and 4 mg/ml, respectively). Both glucose-polymers inhibited mannose receptor-mediated t-PA degradation by human monocyte-derived macrophages in vitro (IC50 7 and 2 mg/ml, respectively). The α2-macroglobulin receptor/low density lipoprotein receptor-related protein (LRP)-mediated t-PA degradation by the macrophages was not affected by dextran. During and after a 45 min infusion of dextran 70 (Macrodex) in rats, in plasma endogenous t-PA concentrations increased to 162 ± 33% and 122 ± 35% respectively. The plasma clearance of a bolus injection of exogenous t-PA was decreased by 33 ± 9% in the same rats.We conclude that dextran inhibits mannose receptor-mediated t-PA clearance. The inhibition of t-PA clearance during dextran infusion results in increased endogenous t-PA plasma concentrations. Increased t-PA concentrations present during thrombus formation are known to increase thrombus lysability. Thus the inhibition of t-PA clearance can contribute to the antithrombotic effect of dextran.

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A one-step enzyme immunoassay for the determination of total tissue-type plasminogen activator (t-PA) antigen in plasma

Cited by 8 publications

References 0 publications

Genetic influences on fibrinogen, tissue plasminogen activatorantigen and von Willebrand factor in males and females

Genetic influences on fibrinogen, tissue plasminogen activatorantigen and von Willebrand factor in males and females

Cartilage degradation and invasion by rheumatoid synovial fibroblasts is inhibited by gene transfer of a cell surface-targeted plasmin inhibitor

Inhibition of Mannose Receptor-mediated Clearance of Tissue-type Plasminogen Activator (t-PA) by Dextran: a New Explanation for Its Antithrombotic Effect

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