A One-Enzyme PCR-RFLP Assay for Identification of Six Medically Important Candida Species

Mirhendi, Hossein; Makimura, Koichi; Khoramizadeh, Mohamadreza; Yamaguchi, Hideyo

doi:10.3314/jjmm.47.225

Cited by 190 publications

(196 citation statements)

References 23 publications

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“…As shown in Table 2, C. albicans alone outnumbered other species and accounted for 73.68% episodes of oral candidiasis. Our findings were consistent with that noted by other investigators [15][16]. Bagg et al [17] showed that patients with advanced cancer have demonstrated a high incidence (51%) of oral colonization with non-C. albicans yeasts [18].…”

Section: Discussionsupporting

confidence: 83%

Oral <i>Candidiasis</i> amongst cancer patients at Qods Hospital

Afraseyabi¹,

Afkhamzadeh²,

Sabori³

et al. 2011

Af J Clin Exp Micro

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Background: Within the past two decades, Candida species have emerged as major human pathogens and are currently the fourth most common cause of nosocomial infection. Propose of this study was to determine the occurrence of oral Candidiasis among cancer patients at Qods hospitals in Sanandaj. Materials and Methods: Sixty cancer patients were examined for oral candidiasis. For all patients, the clinical diagnosis had to be confirmed microbiologically by the presence of yeasts and / or hyphae or pseudohyphae on potassium hydroxidetreated smears of oral swabs. Oral samples were obtained and cultured on Sabouraud's dextrose agar and CHROMagar. Results: 25 out of the 60 patients (41.7%) were males and 35 (58.3%) were females ranging in age from 15 to 79 years. Gastrointestinal cancer and Breast cancer were the most frequent cancer in the studied group, accounting for 65 % and 18.4 % respectively. The mean weight of the patients was 52.67 Kg (range,. Similarly, the mean of hospital stay was 3.58 days (range; 1-9 days). From these patients, 19 Candida spp were isolated; C. albicans alone outnumbered other species and accounted for 73.68% episodes of trash. For C. albicans isolates, the MIC values ranges from 1 to 9 µ g / ml µg / ml for polyenes and from 0.03 to 16 µ g / ml for the azole antifungals. All the Candida albicans had closely related MFCs values. Conclusion: In conclusions, the finding of our study strongly suggest that oral candidiasis is a frequent complication among cancer patients, being C. albicans the main etiological agent.

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Section: Discussionsupporting

confidence: 83%

Oral <i>Candidiasis</i> amongst cancer patients at Qods Hospital

Afraseyabi¹,

Afkhamzadeh²,

Sabori³

et al. 2011

Af J Clin Exp Micro

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“…Candida DNA extraction was performed according to a procedure previously described by Mirhendi et al (14) using phenol-chloroform technique. DNA concentration and purity were determined by measurements at wavelengths of 260 and 280 nm, respectively.…”

Section: Dna Extractionmentioning

confidence: 99%

Molecular Analysis of Candida species with Emphasis on Predisposing Factors in Cutaneous Candidiasis Patients

Ghasemi

Hashemi

Rezaei

et al. 2016

Jundishapur J Microbiol

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Background: Candida species are known as the most common fungal pathogens isolated from clinical specimens that can develop complications ranging from cutaneous to systemic diseases. Objectives: The present study was conducted to evaluate the diversity and distribution of Candida species in various kinds of cutaneous candidiasis in Tehran, Iran. In addition, the critical effects of several predisposing factors on the induction and progression of the disease were considered. Methods: A total of 3000 samples were taken from March 2014 to 2015. The samples were examined using direct microscopy and culturing method. The polymorphism analysis was performed by using polymerase chain reaction -restriction fragment length polymorphism (PCR-RFLP) technique. The internal spacer region (ITS) of the fungal rRNA genes was recruited for PCR amplification of target sequences and Msp1 enzyme was employed to digest PCR amplicons. Results: Out of 3000 samples, yeast was recovered in 290 (9.67%) cases. Onychomycosis was observed predominantly in 164 nail samples (56.5%). The majority of patients were within the age range of 51 -60 years (22.14%). Women working at home (housewives) showed the highest percentage of people at risk (n = 135, 46.5%). Among patients with underlying diseases, diabetic patients with 46 cases (14.2 %) had the highest susceptibility to candidiasis. Genotypic identification showed that Candida albicans is the most common species (n = 132, 45.5 %) recovered from clinical samples, followed by C. parapsilosis (n = 77, 26.5%), C. glabrata (n = 22, 7.5 %), C. krusei (n = 16, 5.5 %), C. tropicalis (n = 37, 12.7 %), and C. guilliermondii (n = 6, 2%). Conclusions:The results of the present study, compared to the previous studies, showed a significantly lower prevalence of cutaneous candidiasis. Among Candida species, C. albicans was still the most common infectious agent isolated from clinical samples. Shifting toward non-albicans Candida species was not confirmed in this study. In addition, our study revealed that there is a direct correlation between some predisposing factors including age of patients, and existence of some non-infectious diseases (e.g. metabolic disorder) and occurrence of Candida infection.

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“…Molecular identification of common Candida species was carried out according to an already described PCRrestriction fragment length polymorphism (RFLP) method (12). Briefly, the ITS1-5.8S rDNA-ITS2 region was amplified using a PCR mixture containing 12.5 µL of 2 × premix (Ampliqon, Denmark), 10 pmol of each ITS1 (5'-TCC GTA GGT GAA CCT GCG G-3') and ITS4 (5'-TCC TCC GCT TAT TGA TAT GC-3') primers (13), 3 µL of extracted DNA, and enough doubledistilled water to obtain a final reaction volume of 25 µL.…”

Section: Methodsmentioning

confidence: 99%

Frequency of Candida Species Isolated from Patients in Children’s Medical Center, Tehran, Iran

Charsizadeh

Nikmanesh

Ahmadi

et al. 2017

Arch Pediatr Infect Dis

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Background: Candida species originating from either endogenous or exogenous sources are one of the main causes of opportunistic infections. Colonization is an important independent risk factor for invasive candidiasis, and many patients admitted to neonatal intensive care unit (NICU) and pediatric intensive care unit (PICU) are colonized with Candida species that may result in invasive candidiasis. Awareness among clinicians about various aspects of colonization is critical to optimal management. The aim of this study was to determine the frequency and species distribution of Candida strains isolated from predisposed patients hospitalized at Children's Medical Center (CMC), Tehran, Iran. Methods: From June 2014 to June 2016, 347 Candida isolates were collected from 341 patients either hospitalized in different wards or referred as outpatients. The yeasts were identified by colony color characteristics using CHROMagar Candida medium and by amplification of the ITS1-5.8S rDNA-ITS2 region in DNA extracted from each isolate followed by analysis of species specific electrophoretic patterns of PCR products digested with the restriction enzyme MspI. Results: Of the 341 patients, 213 were males and 128 were females. Most samples were obtained from the 1 -12-month age group, and the majority of samples represented urine (n = 182), throat swabs (n = 57), and stool samples (n = 53), respectively. The samples were mostly from patients in general wards. The most commonly isolated species was C. albicans (77%), followed by C. tropicalis (8.4%), C. parapsilosis (7.5%), C. glabrata (2.3%), C. kefyr (1.7%), C. krusei (1.1%), C. lusitaniae (0.6%), C. guilliermondii (0.3%), C. albicans + C. parapsilosis (1.4%), and C. albicans + C. glabrata (0.3%). Conclusions: C. albicans is the most common species isolated from children in Iran, followed by C. tropicalis and C. parapsilosis, a prevalence pattern that is relatively different from studies in other countries. Neonates and infants 1 -12 months of age hospitalized in ICU, were more colonized by Candida species than other groups.

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A One-Enzyme PCR-RFLP Assay for Identification of Six Medically Important Candida Species

Cited by 190 publications

References 23 publications

Oral <i>Candidiasis</i> amongst cancer patients at Qods Hospital

Oral <i>Candidiasis</i> amongst cancer patients at Qods Hospital

Molecular Analysis of Candida species with Emphasis on Predisposing Factors in Cutaneous Candidiasis Patients

Frequency of Candida Species Isolated from Patients in Children’s Medical Center, Tehran, Iran

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