2010
DOI: 10.1126/science.1184953
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A NusE:NusG Complex Links Transcription and Translation

Abstract: Bacterial NusG is a highly conserved transcription factor that is required for most Rho activity in vivo. We show by nuclear magnetic resonance spectroscopy that Escherichia coli NusG carboxyl-terminal domain forms a complex alternatively with Rho or with transcription factor NusE, a protein identical to 30S ribosomal protein S10. Because NusG amino-terminal domain contacts RNA polymerase and the NusG carboxy-terminal domain interaction site of NusE is accessible in the ribosomal 30S subunit, NusG may act as a… Show more

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Cited by 301 publications
(446 citation statements)
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“…The maintenance of transcription-translation coupling in a prokaryotic cell would require dynamic inter-regulation between the binding and progression of a pioneer ribosome on the nascent transcript on the one hand and the rate of transcription elongation on the other (9,45); however, the detailed mechanisms by which such regulation is achieved are not known. Furthermore, in bacteria such as Escherichia coli, nascent transcripts that are not simultaneously translated are subject to a mechanism of factor-dependent transcription termination (also referred to as transcriptional polarity) (reviewed in references 1, 6, 15, 40, 44, 47, and 52), so that the occurrence of translation-uncoupled transcription is minimized within the cells (11,43).…”
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confidence: 99%
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“…The maintenance of transcription-translation coupling in a prokaryotic cell would require dynamic inter-regulation between the binding and progression of a pioneer ribosome on the nascent transcript on the one hand and the rate of transcription elongation on the other (9,45); however, the detailed mechanisms by which such regulation is achieved are not known. Furthermore, in bacteria such as Escherichia coli, nascent transcripts that are not simultaneously translated are subject to a mechanism of factor-dependent transcription termination (also referred to as transcriptional polarity) (reviewed in references 1, 6, 15, 40, 44, 47, and 52), so that the occurrence of translation-uncoupled transcription is minimized within the cells (11,43).…”
mentioning
confidence: 99%
“…NusG has two domains that bind Rho and RNA polymerase, respectively (13,36). The Rho-binding domain in NusG has also recently been shown to interact with the S10 protein subunit of the ribosome, which has implications for the mechanism by which translation-uncoupled nascent transcripts may be subject to termination of their synthesis (9). Although null mutations in rho or nusG confer inviability in wild-type E. coli (11), missense mutations in the two genes are known that confer a transcription termination-defective (that is, polarity relief) phenotype (1,12,13,27,36).…”
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“…Translation of a particular codon depends on both the nature and abundance of the respective tRNAs, particularly on the non-random use of synonymous codons and the availability of the respective isoacceptor tRNAs [8]. The overall rate of translation is limited by the codon-specific rates of cognate ternary complex delivery to the A site and is further attenuated by other factors, such as collisions between individual ribosomes in polysomes [3], controlled ribosome stalling (for a recent review, see [9]), or the cooperation between translating ribosomes and the RNA polymerase machinery [4,10].Estimations of error frequencies of translation range between 10 25 and 10 23 , depending on the type of measurement, concentrations and nature of tRNAs that perform misreading, and the mRNA context [11][12][13]. Different approaches were taken to measure these values.…”
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confidence: 99%