A numerical study on the loading of cryoprotectant cocktails-on-a-chip, Part I: Interacting miscible viscous fluids

Scherr, Thomas; Pursley, Shelby; Monroe, W. Todd; Nandakumar, K.

doi:10.1016/j.ijheatmasstransfer.2014.07.026

Cited by 7 publications

(2 citation statements)

References 22 publications

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“…Scherr and co-workers (Scherr et al, 2013) conducted a numerical study of flow field and the concentration distribution during CPA loading into cells in a three inlet T-junction microchannel, and found that each cell has a unique path in the flow field, and thus a distinctive extracellular concentration profile (Figure 7B). For practical use with more realistic and more complicated mixtures of cryoprotectants, they then simulated loading of cryoprotectant cocktails-on-a-chip (Scherr et al, 2014a, b). Data show that the chip provided both controlled loading of CPA to minimize osmotic shock and selective loading of a specific CPA from several to fulfill special requirements.…”

Section: Controllable Addition and Removal Of Cpasmentioning

confidence: 99%

Microfluidics for cryopreservation

Zhao

2017

Biotechnology Advances

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Cryopreservation has utility in clinical and scientific research but implementation is highly complex and includes labor-intensive cell-specific protocols for the addition/removal of cryoprotective agents and freeze-thaw cycles. Microfluidic platforms can revolutionize cryopreservation by providing new tools to manipulate and screen cells at micro/nano scales, which are presently difficult or impossible with conventional bulk approaches. This review describes applications of microfluidic chips in cell manipulation, cryoprotective agent exposure, programmed freezing/thawing, vitrification, and in situ assessment in cryopreservation, and discusses achievements and challenges, providing perspectives for future development.

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Section: Controllable Addition and Removal Of Cpasmentioning

confidence: 99%

Microfluidics for cryopreservation

Zhao

2017

Biotechnology Advances

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“…A few methods of CPA transport have been developed, such as droplet-based methods [12] and channel-based methods [2]. Recently, membrane microfluids have been proposed, which have great potential to solve problems with the cryoprotectant loading/unloading process [1,[13][14][15][16][17]. Zhou et al [11] presented a new approach to achieve safe and efficient loading/unloading of cryoprotectants, namely microfiltration-based sequential perfusion (MSP).…”

Section: Introductionmentioning

confidence: 99%

Numerical Modeling of Heat and Mass Transfer during Cryopreservation Using Interval Analysis

Skorupa

Piasecka-Belkhayat

2020

Applied Sciences

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In the paper, the numerical analysis of heat and mass transfer proceeding in an axially symmetrical articular cartilage sample subjected to the cryopreservation process is presented. In particular, a two-dimensional (axially symmetrical) model with imprecisely defined parameters is considered. The base of the heat transfer model is given by the interval Fourier equation and supplemented by initial boundary conditions. The phenomenon of cryoprotectant transport (Me2SO) through the extracellular matrix is described by the interval mass transfer equation. The liquidus-tracking (LT) method is used to control the temperature, which avoids the formation of ice regardless of the cooling and warming rates. In the LT process, the temperature decreases/increases gradually during addition/removal of the cryoprotectant, and the articular cartilage remains on or above the liquidus line so that no ice forms, independent of the cooling/warming rate. The discussed problem is solved using the interval finite difference method with the rules of directed interval arithmetic. Examples of numerical computations are presented in the final part of the paper. The obtained results of the numerical simulation are compared with the experimental results, realized for deterministically defined parameters.

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Unloading of cryoprotectants from cryoprotectant-loaded cells on a microfluidic platform

Kang

Zou

Qiu

et al. 2017

Biomed Microdevices

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In this paper, a multistep dilution-filtration microdevice (MDFD) is developed for unloading cryoprotectants from cryoprotectant-loaded cells. The MDFD contained a diluent producing region, a dilution-filtration execution region, and a filtrate collection region. It was made of two patterned PMMA stamps with four pieces of sandwiched PVDF membranes. Firstly, the performances of the mixers that were used in the diluent producing region and the dilution-filtration execution region were assessed using fluorescence experiments. Then, the effect of the MDFD structure on the loss of cells was investigated by applying the MDFD to unload glycerin from glycerin-loaded porcine red blood cells. Finally, the effects of the cell density, glycerin concentration, and membrane pore size on the clearance efficiency of glycerin (C ), the survival rate of cells (S ) and the recovery rate of cells (R ) have been studied. Under the designed conditions, C achieved ~80% and S reached ~90%. However, R was only ~40%, mainly resulting from the cells detained on the membrane surface and squeezed through the membrane pores into the filtrate. Increasing the membrane pore size caused high C and S , but low R . For a low glycerin concentration, C , S , and R were all high. For a high cell density, C was high, but both S and R were low. This work is of significance to develop a microfluidic chip for unloading cryoprotectants from a small amount of cryopreserved cell samples.

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A numerical study on the loading of cryoprotectant cocktails-on-a-chip, Part I: Interacting miscible viscous fluids

Cited by 7 publications

References 22 publications

Microfluidics for cryopreservation

Microfluidics for cryopreservation

Numerical Modeling of Heat and Mass Transfer during Cryopreservation Using Interval Analysis

Unloading of cryoprotectants from cryoprotectant-loaded cells on a microfluidic platform

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