Some results for the singular perturbation theory, methods and applications in the new century are reviewed in this paper. It could be found that in the recent decade, many approximate methods have been developed and refined, including the method of averaging, boundary layer methods, methods of matched asymptotic expansion and methods of multiple scales. An overview is given on the new work about various problems, such as the reaction diffusion, turning points, boundary layers, shock waves, stability problem, solitons, attractors, canard solution, scattering light wave and neuron network, etc. And then, a great number of applied problems are solved in applied and astrnatics and so on, from which only some examples are extracted and described in this review.