A numerical analysis of electric field strength over planar microarray dot electrode for dielectrophoretic lab-on-chip device

Yafouz, Bashar; Kadri, Nahrizul Adib; Ibrahim, Fatimah

doi:10.1109/iecbes.2012.6498060

Cited by 7 publications

(8 citation statements)

References 19 publications

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“…In the current study, standard photolithographic processes were used to fabricate a 4 × 4 microarray dot electrode. Yafouz et al 14 described the fabrication process in detail. Similar electrode geometry was used in a previous study designed to characterize cells in homogenous populations 15 .…”

Section: Introductionmentioning

confidence: 99%

Determination of electrophysiological properties of human monocytes and THP-1 cells by dielectrophoresis

Mohamed¹,

Razak²,

Kadri³

2019

Biomed. Res. Ther.

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Introduction: Dielectrophoresis (DEP) is based on polarization and bioparticle movement in applied electric fields. Each type of cells has its own electrical properties within the DEP spectra and undergoes significant changes in direction of frequency following an increase of an applied nonuniform alternating current (AC) electric field. Therefore, DEP can be an effective technique for characterization and separation of different cells. The goal of the current study was to determine the electrophysiological properties of human monocytes and a human monocytic cell line originated from an acute monocytic leukemia patient (THP-1), using a lab on a chip (LOC) device that utilised microarray dot electrodes. Methods: Ten microliters of human monocytes isolated from peripheral blood mononuclear cells and highly confluent THP-1 cells were diluted in DEP medium and added to the spacer of the LOC device. Subsequently, the AC signal in the range of 10 kHz to 2 MHz was supplied to the LOC device, and the dynamic behaviours of monocytes and THP-1 cells due to the DEP force were observed. Images were captured and analyzed using MATLAB software. Results: Microscopic visualization showed that THP-1 cells were physically larger than human monocytes. The dielectric parameters (radius, diameter, and conductivity and permittivity of the cytoplasm and membrane) were greater for THP-1 cells compared to monocytes. The cross-over frequencies for THP-1 cells and monocytes were respectively 66 kHz and 280 kHz. Conclusion: In conclusion, the DEP spectra reflected the morphological differences between monocytes and THP-1 cells. The dielectric properties for each type of cells can be used as the criteria in the development of DEP-based characterization assays.

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Section: Introductionmentioning

confidence: 99%

Determination of electrophysiological properties of human monocytes and THP-1 cells by dielectrophoresis

Mohamed¹,

Razak²,

Kadri³

2019

Biomed. Res. Ther.

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“…We propose adapting this electrode geometry to conduct separation experiments on a mixture of non-homogenous populations using polystyrene particles. In order to avoid the overlapping between the electric fields produced by neighbor dots, a ground plane was introduced in the electrode area between the dot apertures [ 38 ].…”

Section: Introductionmentioning

confidence: 99%

“…The dot electrode geometry possesses several advantages because it has a well-defined and confined region of analysis, effective electric field penetration and an axisymmetrical electric field distribution as shown in electric field simulations in [ 38 ]. As another benefit of this radial electrode geometry, neither field mapping nor image segmentation is required to measure the DEP force on the microparticles.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic Manipulation and Separation of Microparticles Using Microarray Dot Electrodes

Yafouz

Kadri

Ibrahim

2014

Sensors

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This paper introduces a dielectrophoretic system for the manipulation and separation of microparticles. The system is composed of five layers and utilizes microarray dot electrodes. We validated our system by conducting size-dependent manipulation and separation experiments on 1, 5 and 15 μm polystyrene particles. Our findings confirm the capability of the proposed device to rapidly and efficiently manipulate and separate microparticles of various dimensions, utilizing positive and negative dielectrophoresis (DEP) effects. Larger size particles were repelled and concentrated in the center of the dot by negative DEP, while the smaller sizes were attracted and collected by the edge of the dot by positive DEP.

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“…Furthermore, previous simulation works were conducted to explore the electric field over the dot electrode using numerical analysis [74,75], and these works have confirmed its effective electric field penetration.…”

Section: Microarray Dot Electrodementioning

confidence: 92%

“…This electrode design had already been simulated in a previous work [74]. Figure 5 shows the simulation of the electric field strength over the dot electrode with and without ground plane between adjacent dot apertures.…”

Section: Ongoing Researchmentioning

confidence: 99%

Microarray Dot Electrodes Utilizing Dielectrophoresis for Cell Characterization

Yafouz

Kadri

Ibrahim

2013

Sensors

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During the last three decades; dielectrophoresis (DEP) has become a vital tool for cell manipulation and characterization due to its non-invasiveness. It is very useful in the trend towards point-of-care systems. Currently, most efforts are focused on using DEP in biomedical applications, such as the spatial manipulation of cells, the selective separation or enrichment of target cells, high-throughput molecular screening, biosensors and immunoassays. A significant amount of research on DEP has produced a wide range of microelectrode configurations. In this paper; we describe the microarray dot electrode, a promising electrode geometry to characterize and manipulate cells via DEP. The advantages offered by this type of microelectrode are also reviewed. The protocol for fabricating planar microelectrodes using photolithography is documented to demonstrate the fast and cost-effective fabrication process. Additionally; different state-of-the-art Lab-on-a-Chip (LOC) devices that have been proposed for DEP applications in the literature are reviewed. We also present our recently designed LOC device, which uses an improved microarray dot electrode configuration to address the challenges facing other devices. This type of LOC system has the capability to boost the implementation of DEP technology in practical settings such as clinical cell sorting, infection diagnosis, and enrichment of particle populations for drug development.

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A numerical analysis of electric field strength over planar microarray dot electrode for dielectrophoretic lab-on-chip device

Cited by 7 publications

References 19 publications

Determination of electrophysiological properties of human monocytes and THP-1 cells by dielectrophoresis

Determination of electrophysiological properties of human monocytes and THP-1 cells by dielectrophoresis

Dielectrophoretic Manipulation and Separation of Microparticles Using Microarray Dot Electrodes

Microarray Dot Electrodes Utilizing Dielectrophoresis for Cell Characterization

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