A Number of Bone Marrow Mesenchymal Stem Cells but Neither Phenotype Nor Differentiation Capacities Changes with Age of Rats

Tokalov, Sergey V.; Grüner, Susanne; Schindler, Sebastian; Iagunov, Alexey S.; Baumann, Michael; Abolmaali, Nasreddin D.

doi:10.1016/s1016-8478(23)07336-3

Cited by 34 publications

(1 citation statement)

References 22 publications

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“…No correlation was established between these SMSC properties and patient-specific factors such as age or BMI. These observations are consistent with the outcomes from previous studies conducted on both rats and humans, which reported that the growth and differentiation capacity of MSCs in vitro are independent of donor age and weight [ 23 , 24 ].…”

Section: Discussionsupporting

confidence: 92%

Characteristics of the synovial microenvironment and synovial mesenchymal stem cells with hip osteoarthritis of different bone morphologies

Yang,

Koga,

Nakagawa

et al. 2024

Arthritis Res Ther

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Background Variations in bone morphology in patients with hip osteoarthritis (HOA) can be broadly categorized into three types: atrophic, normotrophic, and hypertrophic. Despite the investigations examining clinical elements, such as bone morphology, pain, and range of motion, our understanding of the pathogenesis of HOA remains limited. Previous studies have suggested that osteophytes typically originate at the interface of the joint cartilage, periosteum, and synovium, potentially implicating synovial mesenchymal stem cells (SMSCs) in the process. This study aimed to investigate the potential factors that drive the development of bone morphological features in HOA by investigating the characteristics of the synovium, differentiation potential of SMSCs, and composition of synovial fluid in different types of HOA. Methods Synovial tissue and fluid were collected from 30 patients who underwent total hip arthroplasty (THA) with the variable bone morphology of HOA patients. RNA sequencing analysis and quantitative reverse transcription-polymerase chain reaction (RT-qPCR) were performed to analyse the genes in the normotrophic and hypertrophic synovial tissue. SMSCs were isolated and cultured from the normotrophic and hypertrophic synovial tissues of each hip joint in accordance with the variable bone morphology of HOA patients. Cell differentiation potential was compared using differentiation and colony-forming unit assays. Cytokine array was performed to analyse the protein expression in the synovial fluid. Results In the RNA sequencing analysis, 103 differentially expressed genes (DEGs) were identified, predominantly related to the interleukin 17 (IL-17) signalling pathway. Using a protein–protein interaction (PPI) network, 20 hub genes were identified, including MYC, CXCL8, ATF3, NR4A1, ZC3H12A, NR4A2, FOSB, and FOSL1. Among these hub genes, four belonged to the AP-1 family. There were no significant differences in the tri-lineage differentiation potential and colony-forming capacity of SMSCs. However, RT-qPCR revealed elevated SOX9 expression levels in synovial tissues from the hypertrophic group. The cytokine array demonstrated significantly higher levels of CXCL8, MMP9, and VEGF in the synovial fluid of the hypertrophic group than in the normotrophic group, with CXCL8 and MMP9 being significantly expressed in the hypertrophic synovium. Conclusion Upregulation of AP-1 family genes in the synovium and increased concentrations of CXCL8, MMP9, and VEGF were detected in the synovial fluid of the hypertrophic group of HOA patients, potentially stimulating the differentiation of SMSCs towards the cartilage and thereby contributing to severe osteophyte formation.

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Section: Discussionsupporting

confidence: 92%

Characteristics of the synovial microenvironment and synovial mesenchymal stem cells with hip osteoarthritis of different bone morphologies

Yang,

Koga,

Nakagawa

et al. 2024

Arthritis Res Ther

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Osteogenic ability of rat bone marrow concentrate is at least as efficacious as mesenchymal stem cellsin vitro

et al. 2019

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Cell therapy using bone marrow concentrate (BMC) or purified and expanded mesenchymal stem cells (MSCs) has been shown to have a promising osteogenic capacity. However, few studies have directly compared their relative osteogenic ability. The aim of this study was to compare the osteogenic ability of BMC isolated by density gradient centrifugation with bone marrow‐derived MSCs in vitro using the cells of 3‐month‐old Sprague–Dawley rats. The isolated cells were seeded onto 24‐well plates (1 × 105 cells/well) and cultured in control growth media, osteogenic media with dexamethasone, or media without dexamethasone (which simulated the in vivo tissue environment). Alkaline phosphatase activity at week 2, osteocalcin using quantitative real‐time polymerase chain reaction at week 4, and Alizarin red staining at week 4 were evaluated. In the osteogenic media with dexamethasone, BMC showed equivalent (osteocalcin) or even greater (Alizarin red staining) osteogenic ability compared to MSCs, suggesting that cross‐talk among various cells in the BMC leads to greater osteogenesis. Furthermore, in the osteogenic media without dexamethasone, BMC showed equivalent (osteocalcin) or a trend for greater (Alizarin red staining) bone formation than MSCs alone. Our results suggest that BMC has at least comparable bone regeneration potential to MSCs. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2500–2506, 2019

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Basics of Stem and Progenitor Cells

Harting

2010

Progenitor Cell Therapy for Neurological Injury

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A Number of Bone Marrow Mesenchymal Stem Cells but Neither Phenotype Nor Differentiation Capacities Changes with Age of Rats

Cited by 34 publications

References 22 publications

Characteristics of the synovial microenvironment and synovial mesenchymal stem cells with hip osteoarthritis of different bone morphologies

Characteristics of the synovial microenvironment and synovial mesenchymal stem cells with hip osteoarthritis of different bone morphologies

Osteogenic ability of rat bone marrow concentrate is at least as efficacious as mesenchymal stem cellsin vitro

Basics of Stem and Progenitor Cells

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