A nucleolar protein RRS1 contributes to chromosome congression

Gambe, Arni E.; Matsunaga, Sachihiro; Takata, Hideaki; Ono-Maniwa, Rika; Baba, Atsuomi; Uchiyama, Susumu; Fukui, Kiichi

doi:10.1016/j.febslet.2009.05.033

Cited by 35 publications

(40 citation statements)

References 33 publications

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“…Both Rrs1 and Ebp2 have recently been connected to a role in the progression of mitosis as well. Mybbp1a is phosphorylated by the mitotic kinase Aurora B, and it has been revealed that depletion of Mybbp1a or Rrs1 leads to a mitotic delay and abnormalities in spindle organization (45,68). Additionally, the involvement of Mybbp1a, rpL5, and rpL11 was shown in p53 acetylation and accumulation, which is dependent on the release of Mybbp1a from nucleolar RNA upon stress (54).…”

Section: Discussionmentioning

confidence: 99%

Myb-binding Protein 1a (Mybbp1a) Regulates Levels and Processing of Pre-ribosomal RNA

Hochstatter

Hölzel

Michaela

et al. 2012

Journal of Biological Chemistry

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Background: rRNA gene transcription and processing have to be coordinated, as they are intimately linked. Results: Mybbp1a regulates RNA Pol I transcription and pre-rRNA processing. Conclusion: Mybbp1a coordinates both processes. Significance: Mybbp1a was shown to be an important regulator of cellular proliferation, related to RNA Pol II genes. We link its function now as well to regulating RNA Pol I genes and to control ribosome biogenesis.

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Section: Discussionmentioning

confidence: 99%

Myb-binding Protein 1a (Mybbp1a) Regulates Levels and Processing of Pre-ribosomal RNA

Hochstatter

Hölzel

Michaela

et al. 2012

Journal of Biological Chemistry

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“…Its human orthologue localizes to nucleoli in interphase and at the chromosomal periphery from prometaphase to anaphase (60). Rrs1 depletion by RNAi results in defects in chromosome congression, with mitotic cells showing aberrant chromosome alignment at the metaphase plate (60). The chromosome periphery might then act as a means of transport for various proteins, including nucleolar proteins (61).…”

Section: Discussionmentioning

confidence: 99%

“…Rrs1 is a protein required for ribosome biogenesis in yeast (59). Its human orthologue localizes to nucleoli in interphase and at the chromosomal periphery from prometaphase to anaphase (60). Rrs1 depletion by RNAi results in defects in chromosome congression, with mitotic cells showing aberrant chromosome alignment at the metaphase plate (60).…”

Section: Discussionmentioning

confidence: 99%

Identification of Myb-binding Protein 1A (MYBBP1A) as a Novel Substrate for Aurora B Kinase

Perrera

Colombo

Valsasina

et al. 2010

Journal of Biological Chemistry

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Aurora kinases are mitotic enzymes involved in centrosome maturation and separation, spindle assembly and stability, and chromosome condensation, segregation, and cytokinesis and represent well known targets for cancer therapy because their deregulation has been linked to tumorigenesis. The availability of suitable markers is of crucial importance to investigate the functions of Auroras and monitor kinase inhibition in in vivo models and in clinical trials. Extending the knowledge on Aurora substrates could help to better understand their biology and could be a source for clinical biomarkers. Using biochemical, mass spectrometric, and cellular approaches, we identified MYBBP1A as a novel Aurora B substrate and serine 1303 as the major phosphorylation site. MYBBP1A is phosphorylated in nocodazole-arrested cells and is dephosphorylated upon Aurora B silencing or by treatment with Danusertib, a small molecule inhibitor of Aurora kinases. Furthermore, we show that MYBBP1A depletion by RNA interference causes mitotic progression delay and spindle assembly defects. MYBBP1A has until now been described as a nucleolar protein, mainly involved in transcriptional regulation. The results presented herein show MYBBP1A as a novel Aurora B kinase substrate and reveal a not yet recognized link of this nucleolar protein to mitosis.

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“…By contrast, recent research has revealed that the chromosome periphery has no relationship with chromosome condensation because condensins have been shown to play the main role in chromosome condensation as internal scaffolds (72). In fact, knockdown of a series of CPPs including histone H1.X, nucleolin, NPM and RRS1 does not influence chromosome condensation (18,30,49,60).…”

Section: Cpps Function In Chromosome Alignment Segregation and Spindmentioning

confidence: 87%

“…Knockdown of a variant of histone H1, H1.X, and a centrosomebinding protein, NPM, induces defects in kinetochoremicrotubule attachments similar to nucleolin depletion, resulting in a prolonged time from nuclear envelope breakdown to chromosome alignment (28)(29)(30)82). Depletion of a ribosome biogenesis regulatory protein, RRS1, induces the disappearance of Shugosin 1, which is responsible for centromeric protection (60). This results in premature sister chromatid separation.…”

Section: Cpps Function In Chromosome Alignment Segregation and Spindmentioning

confidence: 99%

The chromosome peripheral proteins play an active role in chromosome dynamics

Matsunaga

Fukui

2010

BioMolecular Concepts

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The chromosome periphery is a chromosomal structure that covers the surface of mitotic chromosomes. The structure and function of the chromosome periphery has been poorly understood since its first description in 1882. It has, however, been proposed to be an insulator or barrier to protect chromosomes from subcellular substances and to act as a carrier of nuclear and nucleolar components to direct their equal distribution to daughter cells because most chromosome peripheral proteins (CPPs) are derived from the nucleolus or nucleus. Until now, more than 30 CPPs were identified in mammalians. Recent immunostaining analyses of CPPs have revealed that the chromosome periphery covers the centromeric region of mitotic chromosomes in addition to telomeres and regions between two sister chromatids. Knockdown analyses of CPPs using RNAi have revealed functions in chromosome dynamics, including cohesion of sister chromatids, kinetochore-microtubule attachments, spindle assembly and chromosome segregation. Because most CPPs are involved in various subcellular events in the nucleolus or nuclear at interphase, a temporal and spatialspecific knockdown method of CPPs in the chromosome periphery will be useful to understand the function of chromosome periphery in cell division.

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A nucleolar protein RRS1 contributes to chromosome congression

Cited by 35 publications

References 33 publications

Myb-binding Protein 1a (Mybbp1a) Regulates Levels and Processing of Pre-ribosomal RNA

Myb-binding Protein 1a (Mybbp1a) Regulates Levels and Processing of Pre-ribosomal RNA

Identification of Myb-binding Protein 1A (MYBBP1A) as a Novel Substrate for Aurora B Kinase

The chromosome peripheral proteins play an active role in chromosome dynamics

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