A nuclear-localized histone-gene binding protein from rice (OsHBP1b) functions in salinity and drought stress tolerance by maintaining chlorophyll content and improving the antioxidant machinery

Lakra, Nita; Nutan, Kamlesh Kant; Das, Priyanka; Anwar, Khalid; Singla‐Pareek, Sneh L.; Pareek, Ashwani

doi:10.1016/j.jplph.2014.11.005

Cited by 66 publications

(69 citation statements)

References 54 publications

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“…Also, histone-binding protein-1b (HBP1b), belonging to the bZIP family of TFs, encoded by SOBIC.009g23000 is induced in sorghum drought-resistant cultivars. In rice, a homolog of this gene is localized within Saltol QTL and its expression is differentially regulated in rice seedlings from contrasting genotypes [46]. Transgenic tobacco plants ectopically expressing OsHBP1b showed better survival under drought and salinity [46].…”

Section: Transcription Factors and Stress Tolerancementioning

confidence: 99%

Transcriptome Analysis of Drought-Resistant and Drought-Sensitive Sorghum (Sorghum bicolor) Genotypes in Response to PEG-Induced Drought Stress

Abdel‐Ghany

Ullah

Ben-Hur

et al. 2020

IJMS

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Drought is a major limiting factor of crop yields. In response to drought, plants reprogram their gene expression, which ultimately regulates a multitude of biochemical and physiological processes. The timing of this reprogramming and the nature of the drought-regulated genes in different genotypes are thought to confer differential tolerance to drought stress. Sorghum is a highly drought-tolerant crop and has been increasingly used as a model cereal to identify genes that confer tolerance. Also, there is considerable natural variation in resistance to drought in different sorghum genotypes. Here, we evaluated drought resistance in four genotypes to polyethylene glycol (PEG)-induced drought stress at the seedling stage and performed transcriptome analysis in seedlings of sorghum genotypes that are either drought-resistant or drought-sensitive to identify drought-regulated changes in gene expression that are unique to drought-resistant genotypes of sorghum. Our analysis revealed that about 180 genes are differentially regulated in response to drought stress only in drought-resistant genotypes and most of these (over 70%) are up-regulated in response to drought. Among these, about 70 genes are novel with no known function and the remaining are transcription factors, signaling and stress-related proteins implicated in drought tolerance in other crops. This study revealed a set of drought-regulated genes, including many genes encoding uncharacterized proteins that are associated with drought tolerance at the seedling stage.

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Section: Transcription Factors and Stress Tolerancementioning

confidence: 99%

Transcriptome Analysis of Drought-Resistant and Drought-Sensitive Sorghum (Sorghum bicolor) Genotypes in Response to PEG-Induced Drought Stress

Abdel‐Ghany

Ullah

Ben-Hur

et al. 2020

IJMS

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“…Electrolyte leakage (EL) indicates damage of the cell membrane and was conducted following the protocol described by Larka et al [79]. The EL was measured using Equation (1): EL (%) = EC 1 /EC 2 × 100 (1)…”

Section: Determination Of Oxidative Stress Markersmentioning

confidence: 99%

Nitrogen Enhances Salt Tolerance by Modulating the Antioxidant Defense System and Osmoregulation Substance Content in Gossypium hirsutum

Sikder

Wang

Zhang

et al. 2020

Plants

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Increasing soil salinity suppresses both productivity and fiber quality of cotton, thus, an appropriate management approach needs to be developed to lessen the detrimental effect of salinity stress. This study assessed two cotton genotypes with different salt sensitivities to investigate the possible role of nitrogen supplementation at the seedling stage. Salt stress induced by sodium chloride (NaCl, 200 mmol·L −1 ) decreased the growth traits and dry mass production of both genotypes. Nitrogen supplementation increased the plant water status, photosynthetic pigment synthesis, and gas exchange attributes. Addition of nitrogen to the saline media significantly decreased the generation of lethal oxidative stress biomarkers such as hydrogen peroxide, lipid peroxidation, and electrolyte leakage ratio. The activity of the antioxidant defense system was upregulated in both saline and non-saline growth media as a result of nitrogen application. Furthermore, nitrogen supplementation enhanced the accumulation of osmolytes, such as soluble sugars, soluble proteins, and free amino acids. This established the beneficial role of nitrogen by retaining additional osmolality to uphold the relative water content and protect the photosynthetic apparatus, particularly in the salt-sensitive genotype. In summary, nitrogen application may represent a potential strategy to overcome the salinity-mediated impairment of cotton to some extent.Plants 2020, 9, 450 2 of 20 most sensitivity to salinity during the seedling growth stage, and remained sensitive at the reproductive stage [9]. Excess salinity inhibits plant growth and development by inducing osmotic stress, particular ion toxicity (Na + and Cl − ), oxidative damage, and/or nutritional disorders in plant tissues, which subsequently lead to weakened plant growth and endurance [10][11][12]. Salt stress has been shown to significantly decrease the uptake and metabolism of numerous mineral nutrients (such as nitrogen, potassium, phosphorus, and calcium), and accelerate the damage of the photosynthetic machinery as well as the whole salt tolerance mechanisms of plants. Salt stress-induced plant metabolism alterations are the secondary consequence of carbon and nitrogen metabolism [13]. Moreover, a high salt concentration perturbs electron transport systems in both chloroplasts and mitochondria [14,15]. This accelerates electron leakage from electron transportation chains and induces the generation of reactive oxygen species (ROS), such as superoxide radicals, hydroxyl radicals, and hydrogen peroxide [16,17]. The over-accumulation of ROS not only damages cellular and biological activities [18][19][20], but also degrades chlorophyll pigments and obstructs the synthesis of proteins, amino acids, lipids, deoxyribonucleic acid, as well as enzymatic and non-enzymatic activities of plants [17]. To avoid ROS-induced oxidative damage, plant-assured endogenous tolerance approaches, such as the antioxidant defense system as well as the accumulation of organic solutes and secondary metabolites [21]....

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“…The protein showed DNA binding property (Uguru et al, 2005; Stead et al, 2006). Nuclear localization indicated it as an essential element of abiotic stress responses (Yadav et al, 2014) like other stress-associated proteins (Tamirisa et al, 2014; Lakra et al, 2015; Wang et al, 2015; Yang et al, 2015). The transgenic tobacco overexpressing SbSI -1 grew normal under control conditions and showed better seed germination, plant growth and biomass as compared to WT plants under stress conditions (Yadav et al, 2014; Udawat et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…The transgenic tobacco overexpressing SbSI -1 grew normal under control conditions and showed better seed germination, plant growth and biomass as compared to WT plants under stress conditions (Yadav et al, 2014; Udawat et al, 2016). A lower degree of chlorophyll bleaching in senescence assay and better retention of photosynthetic pigments under different stresses showed better tolerance of transgenic tobacco (Lakra et al, 2015). The higher cell viability in the transgenic tobacco compared with WT tobacco under stress conditions indicated reduced oxidative damage and better survival of transgenic lines.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of SbSI-1, A Nuclear Protein from Salicornia brachiata Confers Drought and Salt Stress Tolerance and Maintains Photosynthetic Efficiency in Transgenic Tobacco

et al. 2017

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A novel Salicornia brachiata Salt Inducible (SbSI-1) gene was isolated and overexpressed in tobacco for in planta functional validation subjected to drought and salt stress. SbSI-1 is a nuclear protein. The transgenic tobacco overexpressing SbSI-1 gene exhibited better seed germination, growth performances, pigment contents, cell viability, starch accumulation, and tolerance index under drought and salt stress. Overexpression of SbSI-1 gene alleviated the build-up of reactive oxygen species (ROS) and curtailed the ROS-induced oxidative damages thus improved the physiological health of transgenic tobacco under stressed conditions. The higher activities of antioxidant enzymes, lower accumulation of ROS, higher membrane stability, relative water content, and polyphenol contents indicated the better survival of the transgenic tobacco than wild-type (WT) tobacco under stressed conditions. Transgenic tobacco had a higher net photosynthetic rate, PSII operating efficiency, and performance index under drought and salt stress. Higher accumulation of compatible solutes and K+/Na+ ratio in transgenic tobacco than WT showed the better osmotic and redox homeostasis under stressed conditions. The up-regulation of genes encoding antioxidant enzymes (NtSOD, NtAPX, and NtCAT) and transcription factors (NtDREB2 and NtAP2) in transgenic tobacco under stressed conditions showed the role of SbSI-1 in ROS alleviation and involvement of this gene in abiotic stress tolerance. Multivariate data analysis exhibited statistical distinction among growth responses, physiological health, osmotic adjustment, and photosynthetic responses of WT and transgenic tobacco under stressed conditions. The overexpression of SbSI-1 gene curtailed the ROS-induced oxidative damages and maintained the osmotic homeostasis under stress conditions thus improved physiological health and photosynthetic efficiencies of the transgenic tobacco overexpressing SbSI-1 gene.

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A nuclear-localized histone-gene binding protein from rice (OsHBP1b) functions in salinity and drought stress tolerance by maintaining chlorophyll content and improving the antioxidant machinery

Cited by 66 publications

References 54 publications

Transcriptome Analysis of Drought-Resistant and Drought-Sensitive Sorghum (Sorghum bicolor) Genotypes in Response to PEG-Induced Drought Stress

Transcriptome Analysis of Drought-Resistant and Drought-Sensitive Sorghum (Sorghum bicolor) Genotypes in Response to PEG-Induced Drought Stress

Nitrogen Enhances Salt Tolerance by Modulating the Antioxidant Defense System and Osmoregulation Substance Content in Gossypium hirsutum

Overexpression of SbSI-1, A Nuclear Protein from Salicornia brachiata Confers Drought and Salt Stress Tolerance and Maintains Photosynthetic Efficiency in Transgenic Tobacco

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