A Nuclear Calcium-Sensing Pathway Is Critical for Gene Regulation and Salt Stress Tolerance in Arabidopsis

Guan, Qingmei; Wu, Jianmin; Yue, Xiule; Zhang, Yanyan; Zhu, Jianhua

doi:10.1371/journal.pgen.1003755

Cited by 74 publications

(54 citation statements)

References 64 publications

(66 reference statements)

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“…Interestingly, local adaptation to coastal, potentially saline impacted environments has been shown before, where a sodium transporter allele conferred elevated salinity tolerance (Baxter et al, 2010). Since calcium sensing is a crucial pathway for salt stress tolerance in Arabidopsis (Guan et al, 2013), and has shown to be important for salt and drought stress in rice (Oryza sativa) (Xu et al, 2011), natural allelic variation at the CaS gene locus might be one way in which coastal populations adapt their growth to a saline environment. As such, our findings provide an excellent starting point to study the links between calcium signaling-dependent root growth and adaptation to growth in saline environments.…”

Section: Discussionmentioning

confidence: 85%

A Scalable Open-Source Pipeline for Large-Scale Root Phenotyping of Arabidopsis

et al. 2014

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Large-scale phenotyping of multicellular organisms is one of the current challenges in biology. We present a comprehensive and scalable pipeline that allows for the efficient phenotyping of root growth traits on a large scale. This includes a highresolution, low-cost acquisition setup as well as the automated image processing software BRAT. We assess the performance of this pipeline in Arabidopsis thaliana under multiple growth conditions and show its utility by performing genome-wide association studies on 16 root growth traits quantified by BRAT each day during a 5-d time-course experiment. The most significantly associated genome region for root growth rate is a locus encoding a calcium sensing receptor. We find that loss of function and overexpression of this gene can significantly alter root growth in a growth condition dependent manner and that the minor natural allele of the Calcium Sensor Receptor locus is highly significantly enriched in populations in coastal areas, demonstrating the power of our approach to identify regulators of root growth that might have adaptive relevance.

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Section: Discussionmentioning

confidence: 85%

A Scalable Open-Source Pipeline for Large-Scale Root Phenotyping of Arabidopsis

et al. 2014

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“…AtVQ9 protein was exclusively localized in the nucleus, had higher expression at roots and mutation enhanced salt stress tolerance, indicating that AtVQ9 acts antagonistically with AtWRKY8 to mediate salt stress responses (Hu et al 2013). As previously described by Guan et al (2013), AtRSA1 senses salt-induced changes in nuclear free calcium and interacts with a bHLH transcription factor, AtRITF1, which may be phosphorylated by nuclear-localized mitogen-activated protein kinases (MAPKs). The AtRSA1-AtRITF1 complex controls gene expressions which are responsible for Na + homeostasis under salt stress and detoxification of salt-induced reactive oxygen species (Guan et al, 2013).…”

Section: Discussionmentioning

confidence: 87%

“…Previously reported nuclear-localized calcium-binding protein, AtRSA1, was found to regulate the transcription of several genes involved in the detoxification of reactive oxygen species generated by salt stress. It was also found that AtRSA1 (At3g06590) mutant plants were hypersensitive to NaCl but not to LiCl, CsCl, or general osmotic stress (Guan et al, 2013). AtWRKY8 (At5g46350) was mainly responds to high salinity compared to others abiotic stress treatments.…”

Section: Discussionmentioning

confidence: 97%

Paenibacillus yonginensis DCY84T induces changes in Arabidopsis thaliana gene expression against aluminum, drought, and salt stress

Sukweenadhi

Kim

Choi

et al. 2015

Microbiological Research

105

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Current agricultural production methods, for example the improper use of chemical fertilizers and pesticides, create many health and environmental problems. Use of plant growth-promoting bacteria (PGPB) for agricultural benefits is increasing worldwide and also appears to be a trend for the future. There is possibility to develop microbial inoculants for use in agricultural biotechnology, based on these beneficial plant-microbe interactions. For this study, ten bacterial strains were isolated from Yongin forest soil for which in vitro plant-growth promoting trait screenings, such as indole acetic acid (IAA) production, a phosphate solubilization test, and a siderophore production test were used to select two PGPB candidates. Arabidopsis thaliana plants were inoculated with Paenibacillus yonginensis DCY84(T) and Micrococcus yunnanensis PGPB7. Salt stress, drought stress and heavy metal (aluminum) stress challenges indicated that P. yonginensis DCY84(T)-inoculated plants were more resistant than control plants. AtRSA1, AtVQ9 and AtWRKY8 were used as the salinity responsive genes. The AtERD15, AtRAB18, and AtLT178 were selected to check A. thaliana responses to drought stress. Aluminum stress response was checked using AtAIP, AtALS3 and AtALMT1. The qRT-PCR results indicated that P. yonginensis DCY84(T) can promote plant tolerance against salt, drought, and aluminum stress. P. yonginensis DCY84(T) also showed positive results during in vitro compatibility testing and virulence assay against X. oryzae pv. oryzae Philippine race 6 (PXO99). Better germination rates and growth parameters were also recorded for the P. yonginensis DCY84(T) Chuchung cultivar rice seed which was grown on coastal soil collected from Suncheon. Based on these results, P. yonginensis DCY84(T) can be used as a promising PGPB isolate for crop improvement.

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“…The nuclearlocalized calcium-binding protein, RSA1 (SHORT ROOT IN SALT MEDIUM 1), which is required for salt tolerance, interacts with a bHLH transcription factor-RITF1. This interaction was identifi ed by co-immunoprecipitation, and was further validated by BiFC (Guan et al 2013 ). They have shown that RSA1 and RITF1 regulate the transcription of several genes involved in the detoxifi cation of ROS generated by salt stress and that they also regulate the SOS1 gene that encodes a plasma membrane Na + /H + antiporter essential for salt tolerance.…”

Section: Studying Protein-protein Complexesmentioning

confidence: 82%

Towards Understanding Abiotic Stress Signaling in Plants: Convergence of Genomic, Transcriptomic, Proteomic, and Metabolomic Approaches

Soni

Nutan

Soda

et al. 2015

Elucidation of Abiotic Stress Signaling in Plants

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All aspects of a plant's life-beginning with the seed germination and ending with the seed formation-are adversely affected by different abiotic stresses such as salinity, fl ood, drought, heat, cold, etc. Being sessile, plants have developed excellent mechanisms of stress perception and signal transduction. Multiple, complex, and dynamically intertwined interactions among nucleic acids, proteins, and metabolites determine the phenotype and fi nal response of plants towards environmental stresses. In response to these stresses, a multitude of processes are activated which enable the plants to cope with these stresses up to a certain extent. These include alteration of expression of stress-responsive genes, production of stress proteins, alteration of ion transport, activation of various antioxidant systems, and compatible solute accumulation. Our knowledge of abiotic stress signaling has grown in leaps and bounds since the emergence and developments in the omics technologies. Genome-scale studies at transcript, protein, and metabolite levels provide information about dynamic changes taking place at these functional levels. For full understanding of signaling networks, it is essentially important to integrate all these aspects. This approach is of remarkable applicability when the aim is to understand how plants react to abiotic stresses. In order to understand molecular basis of stress tolerance along with signaling network under unfavorable environmental situations, recent progress on systematic use of omics technologies including genomics, transcriptomics, proteomics, and metabolomics has been summarized in this chapter.

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A Nuclear Calcium-Sensing Pathway Is Critical for Gene Regulation and Salt Stress Tolerance in Arabidopsis

Cited by 74 publications

References 64 publications

A Scalable Open-Source Pipeline for Large-Scale Root Phenotyping of Arabidopsis

A Scalable Open-Source Pipeline for Large-Scale Root Phenotyping of Arabidopsis

Paenibacillus yonginensis DCY84T induces changes in Arabidopsis thaliana gene expression against aluminum, drought, and salt stress

Towards Understanding Abiotic Stress Signaling in Plants: Convergence of Genomic, Transcriptomic, Proteomic, and Metabolomic Approaches

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